纳米珍珠粉对大鼠深Ⅱ°烧伤创面愈合的影响

纳米珍珠粉对大鼠深Ⅱ°烧伤创面愈合的影响        作者：崔淑芳，郝光荣，汤 球，孙 伟，曹 平 【摘要】  目的  研究纳米珍珠粉对大鼠深Ⅱ°烧伤创面愈合的影响并与微米珍珠粉在烧伤愈合中进行效比较。  纳米珍珠粉、微米珍珠粉(0.5g/只)分别与甘油按1∶1配制成混合液。以微米珍珠粉组与自然愈合组作对照，纳米珍珠粉组为实验组。测定大鼠14%深Ⅱ°烫伤后7、15、21天的创面愈合率、创面组织学观察以及皮肤羟脯氨酸的含量。结果  纳米珍珠粉组创面愈合率较微米珍珠粉组、自然愈合组高(P＜0.05或P＜0.01)；烧伤后3天各组皮肤羟脯氨酸含量均低于正常(P＜0.01)，纳米珍珠粉组减少程度小于其他组；伤后5天各组HP含量有所恢复，尤以纳米珍珠粉组显著高于自然愈合组及微米珍珠粉组；10天后各组明显升高，而纳米珍珠粉组HP含量的升高差异有非常显著性(P＜0.01)。结论  纳米珍珠粉可以促进大鼠深Ⅱ°烧伤创面的愈合，其愈合作用明显高于微米珍珠粉(P＜0.05)。         【关键词】  纳米珍珠粉；微米珍珠粉；烧伤；羟脯氨酸                   【Abstract】  Objective  To study the efficiency comparison between nano and micro pearl powder for treatment of deep Ⅱ° skin scalds in rats.Methods  Nano and micro pearl powder (0.5g each rat) are mixed with glycerol (1∶1).With the micro pearl powder group and the normal healing group as the control，nano pearl powder were used as drug on the deep Ⅱ° skin scalds of rats in the experimental groups.Wound healing rates was measured at 7，15，21 days post-injury；and the content of hydroxyproline (HP) were detected also.Results  Wound healing rates of the nano pearl powder group were higher than that of the micro pearl powder group and the normal healing group (P＜0.05 or P＜0.01).At the 3th day post-injury，the content of HP in every group were lower than that in the normal group (P＜0.01)，while the decrease in the nano pearl powder group was slight than those in other groups.At the 5th day post-injury，the content of OHP recovered to some extent，with a significantly higher elevation in the nano pearl powder group than those in other groups.At the 10th day post-injury，evident HP increase was found in every group，with a statistical significant higher elevation in the nano pearl powder group (P＜0.01).Conclusion  Nano pearl powder could promote the wound healing of deep Ⅱ° skin scalds in rats significantly，it’s healing was more higher than that of micron pearl powder.         【Key words】  nano pearl powder；micro pearl powder；scald；hydroxyproline(HP)         This research is to study the wound healing efficacy of nano pearl powder in treatment of deep Ⅱ°skin scalds in rats and compare the function with those of micron pearl powder.         1  Material and methods         1.1  Pearl powder  (Zhejiang Fenix Pharmaceutical Co.，Ltd)，nano and micro pearl powder (0.5g each rat ) are mixed with glycerol (1∶1).Dermal hydroxyproline detection kit (Nanjing Jiancheng biotechnology Institute，Approved Number 20040631).         1.2  Experimental animals  Female SD rats (n＝190，body weight 240～260g，Experimental Animal Center，Second Military Medical University).The rats were fedin metabolic cages for 1 week.         Qualified certificate for experimental animal ［SCXK(Hu)2002-0006］；qualified certificate for animal facility ［No.SCXK(Hu)2002-0026］.         1.3  Establishment of deep Ⅱ° skin scalds model in rats  The model was made based on the traditional method set up by Yang Taimei［1］.The rats were denuded with BaS and scalded for 15s in 80℃ water the next day to get deep Ⅱ° skin scalds model in rats with a wound accounting for 14% of the body surface.See fig.1.         1.4  Methods  Those rats were divided into 3 groups after the burn (n=60)：nano pearl powder group，micro pearl powder and normal healing group.In nano pearl powder and micro pearl powder groups the wound were daubed with a mixture of nano，micro pearl powder and glycerol once a day，while the rats in the normal healing group were fed regularly.The experiment lasted 21 days.         1.5  Observations         1.5.1  Wound healing circumstance  The wound development healing percentage and healing circumstance were observed in the whole healing.The healing rates were calculated on day 7、15、21 after scalding.Wound healing rate=healing surface/original skin lesion×100%.Regarding the wound healing as the index，to judge the healing time.         1.5.2  Dermal hydroxyproline(HP)measurement  Tissue in the wound were taken from the 3 groups of rats on day 3，5，10，15 and 21 after scalding with normal rats skin as a control.The dorsal skin were denuded and separated from the connective tissue followed by a drying at 80℃，50mg of which were measured by dermal HP reagent kit.HP content was recorded by μg/mg.         1.6  Data statistics and analysis  Inputting the data into the computer，to analyze the data with the Excel software，a significance dissimilarity of group data was showed P＜0.05 or P＜0.01，the average data was showed x±s.         2  Results         2.1  Comparison of the healing time and the wound development healing rate  The skin wound in the nano pearl powder group is clear with no effusion and infection.In the micro pearl powder group，there is a rat with effusion on the skin surface.In the control group 2 cases of effusion and one infection were found.         The wound healing time of nano pearl powder has a significance dissimilarity more than that of micro pearl powder (P<0.01)，the wound development healing rate is more quickly.(P<0.01，See table 1 for details). Tab.1  Comparison of wound healing time and development healing rate of the different times  (x±s，%)  0Note：compared with control group：*P＜0.05，**P＜0.01；compared with micro pearl powder group：△P＜0.05，△△P＜0.01 2.2 P