安捷伦气质联用仪操作规程

安捷伦气质联用仪操作 Agilent 7890 A/ 5975C气相色谱质谱联用仪操作规程 1. 开机 1)打开载气钢瓶控制阀，设置分压阀压力至0.5Mpa 。 2) 打开计算机，登录进入Windows XP系统，初次开机时使用5975C的小键盘LCP输入IP地址和子网掩码,并使用新地址重起,否则安装并运行Bootp Service 。 3)依次打开7890AGC、5975MSD电源(若MSD真空腔内已无负压则应在打开MSD电源的同时用手向右侧推真空腔的侧板直至侧面板被紧固地吸牢)，等待仪器自检完毕 。 4)桌面双击GC-MS图标，进入MSD化学工作站 5)在上图仪器控制界面下，单击视图菜单，选择调谐及真空控制进入调谐与真空控制界面, 在真空菜单中选择真空状态，观察真空泵运行状态 ，此仪器真空泵配置为分子涡轮泵，状态显示涡轮泵转速涡轮泵转速应很快达到100 %，否则，说明系统有漏气，the neighbouring frequency transmission planning, the system can accommodate more than more than 80 TV channels, 2 reverse channels. Taking into account the needs of e-commerce in the future, this system increase in backhaul bandwidth, take up to 4 channels. Wired TV distribution statistics following table: Wired TV information points distribution schedule-medical floor, rehabilitation floor, 1th, floor, 2nd, floor serial number a layer two layer three layer four layer five layer underground a layer underground two layer total medical floor 53 54 52 71 54 7 5 296 rehabilitation floor 7 5 5 4 4 2 27 2nd, floor 18 18 10 46 1th, floor-East floor 16 16 16 3 51 1th, floor-in the floor 21 15 12 5 53 1th, Floor-West floor 20 15 18 3 56 total 529 (3) information guide and the released system system used concentrated control, and unified management of way will sound video signal, and pictures and scroll subtitles, multimedia information through network transmission to the displayed Terminal, real-time to released visits information, and rehabilitation center image advertising, and medical common sense, and experts introduced, health life, information. and can at any time spots pictures, news, emergency notification, content, main distribution in Center of Hall, hospital handle district, Elevator Hall, public. Systems Management Server security management on the medical floor room, connect to the streaming media over a network control terminals, control terminals and display devices such as LCD TVs, LED screens and other equipment connected, the output information, system support MPEG1/2/4,WMV,AVI and other video format, supports image formats such as JPG,PNG,GIF, support DTS/AC3, MP3 MPEG1/MPEG2 MP3,WMA audio format support slide presentations ( ). Hours second time Kok's public security system ... Information for safe, reliable, and mature application of equipment and advanced technology to the enemy 应检查侧板是否压正、放空阀是否拧紧、柱子是否接好。 2. 调谐 调谐应在仪器至少开机2个小时后方可进行，若仪器长时间未开机为得到好的调谐结果将时间延长至4小时。 1)首先确认打印机已连好并处于联机状态。 2) 在操作系统桌面双击GC-MS图标进入工作站系统。 3)在上图仪器控制界面下，单击视图菜单，选择调谐及真空控制进入调谐与真空控制界面。 4) 单击调谐菜单,选择自动调谐调谐MSD,进行自动调谐,调谐结果自动打印。 5) 如果要手动保存或另存调谐参数，将调谐文件保存到atune.u中。 6) 然后点击视图然后选择仪器控制返回到仪器控制界面。 注意: 自动调谐文件名为ATUNE.U 谱图调谐文件名为STUNE.U 其余调谐方式有各自的文件名. 3. 样品测定 3.1 方法建立 1)7890A配置编辑 点击仪器菜单,选择编辑GC配置进入画面。在连接画面下，输入GC Name:GC 7890A;可在 Notes处输入7890A的配置，写7890A GC with 5975C MSD。点击获得GC配置按钮获取7890A的配置。 the neighbouring frequency transmission planning, the system can accommodate more than more than 80 TV channels, 2 reverse channels. Taking into account the needs of e-commerce in the future, this system increase in backhaul bandwidth, take up to 4 channels. Wired TV distribution statistics following table: Wired TV information points distribution schedule-medical floor, rehabilitation floor, 1th, floor, 2nd, floor serial number a layer two layer three layer four layer five layer underground a layer underground two layer total medical floor 53 54 52 71 54 7 5 296 rehabilitation floor 7 5 5 4 4 2 27 2nd, floor 18 18 10 46 1th, floor-East floor 16 16 16 3 51 1th, floor-in the floor 21 15 12 5 53 1th, Floor-West floor 20 15 18 3 56 total 529 (3) information guide and the released system system used concentrated control, and unified management of way will sound video signal, and pictures and scroll subtitles, multimedia information through network transmission to the displayed Terminal, real-time to released visits information, and rehabilitation center image advertising, and medical common sense, and experts introduced, health life, information. and can at any time spots pictures, news, emergency notification, content, main distribution in Center of Hall, hospital handle district, Elevator Hall, public. Systems Management Server security management on the medical floor room, connect to the streaming media over a network control terminals, control terminals and display devices such as LCD TVs, LED screens and other equipment connected, the output information, system support MPEG1/2/4,WMV,AVI and other video format, supports image formats such as JPG,PNG,GIF, support DTS/AC3, MP3 MPEG1/MPEG2 MP3,WMA audio format support slide presentations ( PPT). Hours second time Kok's public security system ... Information for safe, reliable, and mature application of equipment and advanced technology to the enemy 2)柱模式设定 点击图标，进入柱模式设定画面，在画面中，点击鼠标右键，选择从GC下载方法，再用同样的方法选择从GC上传方法;点击1处进行柱1设定，然后选中On左边方框;选择控制模式，流速或压力。 3)分流不分流进样口参数设定 ,点击图标，进入进样口设定画面。点击SSL-后按钮进入毛细柱进样口设定画面。 ,点击模式右方的下拉式箭头,选择进样方式为不分流方式，分流比为50:1，在空白框内输入进样口的温度为220?，然后选中左边的所有方框。 ,选择隔垫吹扫流量模式标准，输入隔垫吹扫流量为 3ml/min。对于特殊应用亦可选择可切换的，进行关闭。 4)柱温箱温度参数设定 点击图标，进入柱温参数设定。选中柱箱温度为开左边的方框;输入柱子的平衡时间为0.25分钟。 5)数据采集方法编辑 从方法菜单中选择编辑完整方法项，选中除数据分析外的三项，点击确定。编辑关于该方法的注释，然后点击确定。 3.1.2 编辑扫描方式质谱参数 1)点击图标编辑溶剂延迟时间以保护灯丝，调整倍增器电压模式(此仪器选用增益系数)，选择要使用的数据采集模式，如全扫描、选择离子扫描等。 the neighbouring frequency transmission planning, the system can accommodate more than more than 80 TV channels, 2 reverse channels. Taking into account the needs of e-commerce in the future, this system increase in backhaul bandwidth, take up to 4 channels. Wired TV distribution statistics following table: Wired TV information points distribution schedule-medical floor, rehabilitation floor, 1th, floor, 2nd, floor serial number a layer two layer three layer four layer five layer underground a layer underground two layer total medical floor 53 54 52 71 54 7 5 296 rehabilitation floor 7 5 5 4 4 2 27 2nd, floor 18 18 10 46 1th, floor-East floor 16 16 16 3 51 1th, floor-in the floor 21 15 12 5 53 1th, Floor-West floor 20 15 18 3 56 total 529 (3) information guide and the released system system used concentrated control, and unified management of way will sound video signal, and pictures and scroll subtitles, multimedia information through network transmission to the displayed Terminal, real-time to released visits information, and rehabilitation center image advertising, and medical common sense, and experts introduced, health life, information. and can at any time spots pictures, news, emergency notification, content, main distribution in Center of Hall, hospital handle district, Elevator Hall, public. Systems Management Server security management on the medical floor room, connect to the streaming media over a network control terminals, control terminals and display devices such as LCD TVs, LED screens and other equipment connected, the output information, system support MPEG1/2/4,WMV,AVI and other video format, supports image formats such as JPG,PNG,GIF, support DTS/AC3, MP3 MPEG1/MPEG2 MP3,WMA audio format support slide presentations ( PPT). Hours second time Kok's public security system ... Information for safe, reliable, and mature application of equipment and advanced technology to the enemy 2)编辑SIM方式参数点击参数编辑选择离子参数，驻留时间和分辨率参数适用于组里的每一个离子。在驻留列中输入的时间是消耗在选择离子的采样时间。它的缺省值是100 毫秒。它适用于在一般毛细管GC峰中选择2-3个离子的情况。如果多于3个离子，使用短一点的时间(如30或50毫秒)。加入所选离子后点击添加新组，编辑完SIM参数后关闭。 3.2 采集数据 1)点击GC-MS图标，在方法文件夹中选择所要的方法。 2)选好方法后，点击图标, 依次输入文件名;操作者;样品名等相关信息,完成后按确定键，待仪器准备好后进样的同时按GC面板上的Start键，以完成数据的采集。 3) 当工作站询问是否取消溶剂延迟时,回答NO或不选择。如果回答YES，则质谱开始采集，容易损坏灯丝。 3.3 数据分析 1) 点击GC-MS数据分析图标，点击下图中文件调入数据文件。 2)在全扫描方法中要得到某化合物的名称，先右键双击此峰的峰高，然后在右键双击峰附近基线的位置得到本底的质谱图，然后在菜单文件下选择背景扣除即可得到扣除本底后该化合物的the neighbouring frequency transmission planning, the system can accommodate more than more than 80 TV channels, 2 reverse channels. Taking into account the needs of e-commerce in the future, this system increase in backhaul bandwidth, take up to 4 channels. Wired TV distribution statistics following table: Wired TV information points distribution schedule-medical floor, rehabilitation floor, 1th, floor, 2nd, floor serial number a layer two layer three layer four layer five layer underground a layer underground two layer total medical floor 53 54 52 71 54 7 5 296 rehabilitation floor 7 5 5 4 4 2 27 2nd, floor 18 18 10 46 1th, floor-East floor 16 16 16 3 51 1th, floor-in the floor 21 15 12 5 53 1th, Floor-West floor 20 15 18 3 56 total 529 (3) information guide and the released system system used concentrated control, and unified management of way will sound video signal, and pictures and scroll subtitles, multimedia information through network transmission to the displayed Terminal, real-time to released visits information, and rehabilitation center image advertising, and medical common sense, and experts introduced, health life, information. and can at any time spots pictures, news, emergency notification, content, main distribution in Center of Hall, hospital handle district, Elevator Hall, public. Systems Management Server security management on the medical floor room, connect to the streaming media over a network control terminals, control terminals and display devices such as LCD TVs, LED screens and other equipment connected, the output information, system support MPEG1/2/4,WMV,AVI and other video format, supports image formats such as JPG,PNG,GIF, support DTS/AC3, MP3 MPEG1/MPEG2 MP3,WMA audio format support slide presentations ( PPT). Hours second time Kok's public security system ... Information for safe, reliable, and mature application of equipment and advanced technology to the enemy 质谱图，最后右键双击该质谱图 ，便得到此化合物的名称。 3)用鼠标右键在目标化合物TIC谱图区域内拖拽可得到该化合物在所选时间范围内的平均质谱图，右键双击则得到单点的质谱图。 4)在选择离子扫描方法中不需要背景扣除操作。 3.3 定量 定量是通过将来自未知量化合物的响应与已测定化合物的响应进行比较来进行的。 手动设置定量数据库 1)选择校正/设置定量访问定量数据库全局设置页。 2)手动检查由测定样品数据文件生成的色谱图。 3) 通过单击色谱图中化合物的峰来分别选择每种化合物。 4) 在显示的谱图中选择目标离子。 5)选择此化合物的限定离子。 6)给化合物命名，如果此化合物是内标，则应标识。 the neighbouring frequency transmission planning, the system can accommodate more than more than 80 TV channels, 2 reverse channels. Taking into account the needs of e-commerce in the future, this system increase in backhaul bandwidth, take up to 4 channels. Wired TV distribution statistics following table: Wired TV information points distribution schedule-medical floor, rehabilitation floor, 1th, floor, 2nd, floor serial number a layer two layer three layer four layer five layer underground a layer underground two layer total medical floor 53 54 52 71 54 7 5 296 rehabilitation floor 7 5 5 4 4 2 27 2nd, floor 18 18 10 46 1th, floor-East floor 16 16 16 3 51 1th, floor-in the floor 21 15 12 5 53 1th, Floor-West floor 20 15 18 3 56 total 529 (3) information guide and the released system system used concentrated control, and unified management of way will sound video signal, and pictures and scroll subtitles, multimedia information through network transmission to the displayed Terminal, real-time to released visits information, and rehabilitation center image advertising, and medical common sense, and experts introduced, health life, information. and can at any time spots pictures, news, emergency notification, content, main distribution in Center of Hall, hospital handle district, Elevator Hall, public. Systems Management Server security management on the medical floor room, connect to the streaming media over a network control terminals, control terminals and display devices such as LCD TVs, LED screens and other equipment connected, the output information, system support MPEG1/2/4,WMV,AVI and other video format, supports image formats such as JPG,PNG,GIF, support DTS/AC3, MP3 MPEG1/MPEG2 MP3,WMA audio format support slide presentations ( PPT). Hours second time Kok's public security system ... Information for safe, reliable, and mature application of equipment and advanced technology to the enemy 7)将此化合物的谱图保存至定量数据库中。 8)对希望添加到定量数据库的每种化合物重复步骤2至7。 9) 如果已添加完需要的所有化合物，则选择校正/编辑化合物以查看完整列。 4. 关机 在操作系统桌面双击GC-MS图标进入工作站系统进入调谐和真空控制界面选择放空，在跳出的画面中点击确定进入放空程序。 本仪器采用的是涡轮泵系统，需要等到涡轮泵转速降至10%以下，同时离子源和四极杆温度降至100?以下，大概40分钟后退出工作站软件，并依次关闭MSD、GC电源，最后关掉载气。 the neighbouring frequency transmission planning, the system can accommodate more than more than 80 TV channels, 2 reverse channels. Taking into account the needs of e-commerce in the future, this system increase in backhaul bandwidth, take up to 4 channels. Wired TV distribution statistics following table: Wired TV information points distribution schedule-medical floor, rehabilitation floor, 1th, floor, 2nd, floor serial number a layer two layer three layer four layer five layer underground a layer underground two layer total medical floor 53 54 52 71 54 7 5 296 rehabilitation floor 7 5 5 4 4 2 27 2nd, floor 18 18 10 46 1th, floor-East floor 16 16 16 3 51 1th, floor-in the floor 21 15 12 5 53 1th, Floor-West floor 20 15 18 3 56 total 529 (3) information guide and the released system system used concentrated control, and unified management of way will sound video signal, and pictures and scroll subtitles, multimedia information through network transmission to the displayed Terminal, real-time to released visits information, and rehabilitation center image advertising, and medical common sense, and experts introduced, health life, information. and can at any time spots pictures, news, emergency notification, content, main distribution in Center of Hall, hospital handle district, Elevator Hall, public. Systems Management Server security management on the medical floor room, connect to the streaming media over a network control terminals, control terminals and display devices such as LCD TVs, LED screens and other equipment connected, the output information, system support MPEG1/2/4,WMV,AVI and other video format, supports image formats such as JPG,PNG,GIF, support DTS/AC3, MP3 MPEG1/MPEG2 MP3,WMA audio format support slide presentations ( PPT). Hours second time Kok's public security system ... Information for safe, reliable, and mature application of equipment and advanced technology to the enemy