SHR大鼠延髓腹外侧头端区血管紧张素_的作用研究_英文_

SHR大鼠延髓腹外侧头端区血管紧张素_的作用研究_英文_ () 文章编号: 100820872 19990320219205 大鼠延髓腹外侧头端区血管紧张素? 的作用研究SH R 赖奕木, 陈达光, 吴可贵, 戴秀中 () 福建省高血压研究所, 福建医科大学附属第一医院, 福州 350005 ( ) 摘要: 探讨 SH R 及W KY 大鼠延髓腹外侧头端区 ro st ra l ven t ro la te ra l m edu lla, RV LM 内微量注射血管紧 1 8 () 张 素 和血管紧张素 受体阻滞剂 [ , ]对心血管活动的影响。 在 及大 ?? ?? A ng Sa rT h rA ng SH R W KY (鼠, 微量注射人工脑脊液, 不引起明显的心血管效应。内微量注射递增剂量的 :? RV LM RV LM A ng pm o l ) 011, 110, 10, 100, 引起血压升高, 心率增加。两组实验动物血压、心率的变化无统计学差别。的效应可 ? A ng 1 8 1 8 (被预先注射 [ , ]所阻断。 相反, 微量注射剂量递增的 [ , ]: 011,? ? Sa rT h rA ng RV LM Sa rT h rA ng pm o l ) 110, 10, 100, 引起血压下降, 心率减慢。在 大鼠, 血压下降的幅度明显大于大鼠, 而心率的变化在 SH R W KY 两组动物中无显著差别。实验结果提示大鼠 内 具有升高血压和增加心率的作用。的作? ? RV LM A ng A ng 用与 A ng 受体介导有关。SH R 大鼠 RV LM 内神经元对内源性 A ng 的敏感性增高。SH R 大鼠高血压的? ? 形成与 内 ? 的升压作用增强有关。RV LM A ng 关键词: 延髓腹外侧头端区; 血管紧张素? ; 高血压; SH R 大鼠 中图分类号: 54411; 954152; 9513 文献标识码: RQQA CA RD IO VA SCUL A R EFFECTS O F M ICRO INJECT IO N O F ? A NG IO TENS IN A ND ITS A NTA GO N IST INTO THE RO STRAL VENTROL A TERAL M ED ULL A O F SPO NTA - NEO USLY HY PERTENS IVE RA TS 2222, , , L A I Y im u CH EN D agu an gW UK egu iDA I X iu zho n g (), , 350005 F uj ian I ns t itu te of H y p e r tens ion F irs t A f f il ia ted H osp ita l of F uj ian M ed ica l U n iv e rs ity F u z h ou () () A bstra c t: T h e m ean a r te r ia l b loo d p re ssu re M A P and h ea r t ra te H R re spo n se s w e re m ea su red by m i2 1 8 () , , ], ? ? ? c ro in jec t ing o f inc rea sing do se s o f ang io ten sin A ng and it s an tago n istSa rT h rA ng in to th e () () () 2ro st ra l ven t ro la te ra l m edu lla RV LM o f spo n taneo u sly h yp e r ten sive ra t s SH R and W ista rKyo to W KY () . : 011, 110, 10, 1002? ra t sM ic ro in jec t io n o f inc rea sing do se s o f A ng pm o lin to th e RV LM cau sed a do sede2 . p enden t p re sso r and tach yca rd iac re spo n se s in SH R and W KY ra t sT h e inc rea se s in bo th M A P and H R w e re 1 8 . [ , ]? o f sim ila r m agn itude in th e tw o st ra in sP re t rea tm en t o f th e RV LM w ith Sa rT h rA ng abo lish ed th e 1 8 . , [ , ] ?M A P and H R re spo n se s p ro duced by m ic ro in jec t io n o f A ng In co n t ra stm ic ro in jec t io n o f Sa rT h r 收稿日期: 1998209204 修回日期: 1999201226 基金项目: 国家自然科学基金资助项目 3880409 ( ) 作者简介: 赖奕木 19692, 男, 福建仙游人, 原福建医科大学附属第一医院心内科主治医师, 硕士, 现为湖北医科大学介入心 血管学专业博士研究生, 从事血压的神经调节机制研究 1 () ? : 011, 110, 10, 1002. A ng pm o le lic ited a do sedep enden t dep re sso r and b radyca rd ic re spo n se sT h e m agn i2 () < 0101, tude o f th e M A P re spo n se s w a s sign if ican t ly g rea te r P in SH R th an th a t in W KY ra t sw h ile th e . m agn itude o f H R re spo n se s w a s no t sign if ican t ly d iffe ren t in th e tw o sta in sT h e se re su lt s ind ica ted th a t A ng ? ac t s a s an exc ita to ry agen t in th e reg io n o f RV LM to m o du la te th e ca rd io va scu la r ac t iv it ie s in SH R and . ? W KY ra t sT h e ca rd io va scu la r effec t s o f A ng m ay be re la ted to th e ac t io n o f it s sp ec if ic recep to r s in th e . . ? RV LM o f ra t sT h e ac t iv ity o f A ng in th e RV LM o f SH R is h igh e r th an th a t o f W KY ra t sT h is inc rea sed ? ac t io n o f A ng m ay co n t r ibu ted to th e gene ra t io n and m a in tenance o f h igh e r b loo d p re ssu re in th is m o de l o f .h yp e r ten sio n spo n taneo u sly h yp e r ten sive Key s: ro st ra l ven t ro la te ra l m edu lla; ang io ten sin ? ; h yp e r ten sio n; ra t s () t io n w ith th e h ead f ixed in a ste reo tax ic f ram e , T h e ro st ra l ven t ro la te ra l m edu lla RV LM a ()2, . reg io n k now n to p lay a c r it ica l ro le in p ro v id ing th e W D T V X i’an In st rum en t s T h e t rach ea and , sym p a th e t ic d r ive and m a in ta in ing th e a r te r ia l b loo d e sop h agu s w e re t ran sec tedand th e d ista l t rach ea 1 () . w a s cannu la ted to fac ilita te ven t ila t io nT h e infe r io r B P , h a s been sugge sted to be a new p re ssu re 5o cc ip ita l bo ne w a s ca refu lly rem o ved to p ro v ide a 2po ten t ia l site o f ac t io n o f th e b ra in ren in ang io ten sin 2 (, 6 . . mm w indow to th e su rface o f th e RV LM In? sy stem A h igh den sity o f ang io ten sin A ng3 4 , ) th e exp e r im en t sw e inve st iga ted th e ac t io n o f veh i2 ? ? and A ng b ind ing site sh a s been dem o n2 1 () 22, , , [ ? c leL G lu tam a te L G lu A ng and Sa r, st ra ted in th e RV LM o f ra t sind ica t ing a po ssib le 8 ] . ?T h rA ng R a t s b rea th ed room a ir spo n ta2 ? . ac t io n o f b ra in A ng o n RV LM neu ro n sD a ta a l2 .so sugge st th a t re spo n sivene ss o f neu ro na l e lem en t s neo u sly o f th e RV LM to th e exc ita to ry d r ive p ro v ided by ( ) 12 2110 ,M icro in jec t ion techn ique L G lu nm o l 5 1 8 . ? A ng m ay be a lte red in SH R In th e p re sen t () : 011, 110, 10, 100[ , ] ? A ng pm o land Sa rT h r study w e inve st iga ted th e ca rd io va scu la r effec t s o f () : 011, 110, 10, 100? A ng pm o lw e re d isso lved in 1 , , ? m ic ro in jec t io n o f A ng and it s an tago n istSa r( , :ƒa r t if ic ia l ce reb ro sp ina l f lu id aC SF in mm o lL 8 ] ? , T h rA ng in to th e RV LM o f spo n taneo u sly 13. 3 , 314 , 1. 3 , 112 , 016N aC lKC lC aC l2 M gC l2 () ()2h yp e r ten sive ra t s SH R and W ista rKyo to W KY , 32. 0 , 314 , N aH CO 3 N aH CO 3 and g luco sepH .ra t s ) 712, 011 5 and in jec t io n s in vo lum e o f Λl a t seco nd . 2 in te rva lM ic ro in jec t io n s w e re m ade f rom do ub le ba r re lled g la ss m ic rop ip e t te s w ith a t ip d iam e te r s o f 1 M a ter ia ls an d m e thod s 100 111 2. Gen era l procedure s A ll exp e r im en t s w e re ΛmIn jec t io n s w e re m ade w ith a se lfm ade 2. 30 1622h andh e ld sy r inge rA m in im um o f m in w a s a l2 p e rfo rm ed in w eek o ld m a le SH R and age ..low ed be tw een in jec t io n s m a tch ed m a le W KY ra t s A n im a ls w e re ane s2 (13 )1112 ƒIden t if ica t ion of the RVLM T h e RV LM w a s , . . . th e t ized w ith u re th ane gk gipT h e rec ta l 3710, 3810tem p e ra tu re w a s de tec ted and k ep t a t 10 2iden t if ied by in jec t io n o f nm o l o f L G lu in to th e () ?. , A fem o ra l a r te r ia l p re ssu re B P m ean a r te r ia l . , RV LM In o u r exp e r im en t sth e RV LM w a s coo r2 18, 410 3d ina ted mm ro st ra l to th e o cc ip ita l fo ra2 () () p re ssu re M A P and h ea r t ra t H R w e re reco rded 17, 119 016,, 1m en mm la te ra l to th e m id line and (6200, N IHON , synch ro no u sly o n a po lyg rap h RM018 .mm deep f rom th e ven t ra l su rface ).J ap an 114 ,H isto logy A t th e end o f each exp e r im en t A ne sth e t ized ra t s w e re p laced in a sup ine po si2 ? 6 015 ? 013 16 2 th e lo ca t io n o f th e p ip e t te t ip w a s m a rk ed by in jeck P a and bpm bpm befo re v s k P a 11 15% . 00t io n o f Λl o f fa st g reenT h e depo sit io n o f )6 ?2 , ; = 6. k P a and bpm bpm af te rre sp ec t ive lyn In 40 th e dye w ith in th e RV LM w a s exam ined in Λm , 5 co n t ra stin jec t io n s o f aC SF veh ic le m in befo re . se r ia l sta ined w ith N eu t ra l redM ic ro in jec t io n site s 2 A ng h ad no effec t s o n th e M A P o r H R re spo n s?w e re iden t if ied by th e depo sit io n o f N eu t ra l red and .e s refe r red to standa rd ana tom ica l st ruc tu re s o f th e RV LM acco rd ing to th e a t la s o f P ax ino s and 6 W a sto n . θ 115 Sta t ist ica l ana ly s is D a ta a re exp re ssed a s x ?. 2sT h e unp a ired S tuden t’ s tte st w a s u sed to com 2 p a red th e m agn itude o f M B P and H R re spo n se s to . each d rug in jec t io n be tw een SH R and W KY ra t sA < 0105 va lue o f P w a s co n side red sta t ist ica lly sign if2 ican t. 2 Re sults M ic ro infec t io n s o f aC SF in to th e RV LM o f SH R and W KY ra t s h ad neg lig ib le effec t s o n B P and . ? H RM ic ro in jec t io n s o f d iffe ren t do se o f A ng in2 () < 01052 to th e RV LM e lic ited sign if ican t ly P do se dep enden t inc rea se s in bo th M A P and H R in SH R ()1. and W KY ra t s F ig T h e p re ssu re began to in2 5 , c rea se w ith in s af te r th e in jec t io n reach ed a p eak 2 , 5, 15 w ith in m in and rem a ined e leva ted fo r m in ( )2. F ig A T h e ch ange in M A P and H R w e re no t .sign if ican t ly d iffe ren t be tw een SH R and W KY ra t s 1 8 L ine g rap h s show com p a red effec t s o f inc rea sing F ig 1 F ig 3 show th a t [ Sa r, T h r] A ng m ic ro in2 ? ? do se s o f A ng o n m ean a r te r ia l p re ssu re (