牛性别鉴定后IVP胚胎冷冻技术研究

牛性别鉴定后IVP胚胎冷冻技术研究 IVP????J?J????'?,?? !?????ntLI2?<?4??nt#?<?????nt????nt??qunt (1#S<???!'?????o?????'<Y??#S<??V100081 2??#SJ?<u?f??q?????????????#S?IJ?<u?f?????C#T071001) ?????l?????????fqc?????MIVP??????J?J?????#???J????'???+?,???.LV?????' ?D??J?J?J??]???.???????fqc?????MIVP?????????5???>??LV??????????<::1LI?fqc???? ?MIVP????<5???x?N??J?J?????J?J?LV?x1.5MEGJ?J?q6J?J????????.??J???J?24?E ???MJ?????<?;?x????A,BJ?#???qH??!#J?#K???????CJ?!???LV2LI?fqc????IVP?????x OPS????#OJ?J??.???????M?.OPS????#OJ?J?<M??????????!#?O??J????7?-?????V(P> 0.05)LV???MOPS????#OJ?J???IVP?fqc?????????'#??+??LV J??w????:IVP;J?J?;?fqc????;????#O StudiesonCryopreservationofsexedIVPEmbryosincattle Yuwenli'sLishujing',zFujingtaontBaiyinntHugangnt (1BeijingAnboEmbryoBiotechCenter,80XueYuanNanLu.HaiDian District,Beijing,10081) CollegeofAnimalScienceandTechnology,AgriculturalUniverstityof HeBei,WestCampus;071001) Aboir`t:Theobjectiveofthisstudywastoimprovethecryopreservativeoffectandpre- ganacyrateonbovinesexedIVPembryos.Theresultsasfollows:Ltheconventionalmethod wasnotfeasibletosexedIVPembryos,ThesurvivalrateofsexedIVPembryosfrozenwith 1.5MEGwas0%after24hculture.theviabilityrateofEmbryosfrozenwithdemiembryo cryoprotectantwhichproducedbyA.Btechnology,wasafter24hand48hcuiture.2.there werenodifferencesbetweenvitrificationincombinationwithOPSmethodandcontrolwhich consistedoffreshsexedIVPembryos(P>0.05).ItwasconcludedthatbovinesexedIVPem - bryoswerecouldbesuccessfullyfrozenbyvitrificationincombinationwithOPSmethods. <T#??..??:bovine;IVP;cryopreservationsexed;vitrfication 1q/?? ???R<u?f?f???C!#?O#????????o<???<U?????? !??(??<?: !?????#S<???!'?????o?????'<Y???????,???? ?'???7?8?V??????J?J??:??[1,21?.???.?X?~qH<??fqc ????!#?O???????o<??????xLV??<T??!#?O????<M!# J??????????x???u????<5?.[3.41?.#(??????J??c?7?? ?u???V???.?????x#???[5.61?.??q???????[51?.???f??#??? ?P???.?A?????o???o?????????!?o?fu?.????J?!?#?!n ???%??????:laotouxie@sohu.com?Odairyguide@163.com t91<?#??x?????????z??<)?????V[10,11,121LV?? <?<5?.#????'?????????????V?[?n?Gt's,'41?.???'???? ???V????!#?O#?????????J?J??7???????!?o?f[15,'61 ??1973??WilmutIetalt"Iqm?x???o???Gq??L J?J?????????<???<????.<O?+?2?L????????!#?O#? ??????J?J??]?????+<??????,???.!R?f?u?'<????o ?:??#O????J?J?<T<U?.???????7?????1???????5?] ??LV1985??Rall??Fahyt"l#??M<??x???7J???????J? <:????#OJ?J??????????????.????#O?'<?<b???1?? ?:?.?#?????x???oJ?J???#??G<?????????????#O?? ?I?.?x???????I#O??!#???.??????????J?J????????G (191.????J?J??????x?o<?<Y???x?.J??7<5??????<??? ???0???????'?.?????G?l?.???I?????=!>??[201 1997??VanWagtendonk-dealE2']?1????#O?? ??J?J????'?x<??f???C!#J??o<?<Y?.???}?'?????5 #?!#393?z?.????44.5%?????5???n???.<M!n?????? ??J?J?????45.1%(n=335)?????5???n?????7???VLV O'Kearney-FlynnMelalt}1qm?x????#OJ?J????'J?J? 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