MTT法检测国产PGA支架对人成纤维细胞增殖的影响

MTT法检测国产PGA支架对人成纤维细胞增殖的影响 MTT法检测国产PGA支架对人成纤维细胞 增殖的影响 JournalofTissue EngineeringandReconstructiveSurgery?eFebruary?g2006V01?g2No1 MTT??????????PGA?z?? ?????R?????????????????? ???)?????????s???z?2?P?S?????i?? ???????? CR????Cc????????????PGA?z?????9??CJ???????????R??????????????????Co???????F???????9??CJ?????????? ?R?????????e???U?????????????C96??L???e???E?e??????????PGA?z?????????????e?HMTT???????R?????????H???? PGA?i??????????Co?????E?e?????????????9??CJ???????????R???????????P???????????g?8???oCo????????PGA ?z?????9??CJ???????????R?????????????????g?8?????e???????????H?????R?????????7??????????????Co CR???,??Cc?9???R?????????????R?????????????R???????????????? MTTAssayForTheInfluenceOfPGASoakageOnHumanFibroblastProliferationDENGDan?gWUXiaoli?eXU Feng,GA0zhen?eLIUWei,CUILei?eCA0Yilin?gDepartmentofPlasticandReconstructiveSurgery,ShanghaiNinthPeopleG0S Hospital?eShanghaiJiaoTongUniversitySchoolofMedicine?eShanghaiTissueEngineeringResearchandDevelopmentCenter, Shanghai200011?eChina?g CRAbstractCcObjectiveToexaminewhetherdomestic-madePGAscaffoldaffectshumantennocyte?ekeloidandskin fibroblastproliferation?gMethodsHumantennoeytes?gkeloidandskinfibroblastswerecul turedinvitroto passagetwoand seededon96??wellplate?eculturedwithdifferentPGAscaffoldsoakages?eandtheireffects Onfibroblastproliferationwere examinedbyMT-Fassay?gResultsTherewasnodifiefenceamongcellgrowthcurvesoffibro blastsculturedbydifferent soakages?gConclusionDomesticGSmadePGAscaffolddoesnotaffecthumantennocyte?g keloidandskinfibroblastproliferation andcanbeusedfortissueengineeringwithfibroblastsasseedcell?g CRKeywordsCcKeloidfibroblast?VDermalfibroblast?VTennocyte?VPolyglycolicacid (PGA) PGA?z???z?????????????P???z???????e???? ?P???????k???e???????e???????H???????????e???Y ?????????????g???R???k???9?????e?G?R???????P ???E?m?????k?????e?(???T?????????k?e?6???????? ?P???????????H?g?P?????X??????Co???F?e?(?H?? PGA?6?7?????e?????3?o?w?e?????H?u?????C???? ?????-Co?U???????H????????????????PGA?z???? ???9??CJ???????????R???????????????????e?7???R ?g?????????7????????CJ????PGA?7?z???????????? ?????S?????????+Co 1?????g???? 1?g1?????????????????-?l ?????z?X???u???9?????m??????CJ?????e?a?W?? ????27?????kCJ22???K?k??19?????k???n?e???P?a ???x???b?g?????g0?g25?n?z?T?G????????20?a????30 ???n?????6200011?i???o?i?????6?k???5?????~???U???? ?c?5?????E?F???6?i?????????s?S???g?????X?? ?a??Co?????9?????m????????????????CJ?????o???? ?????????????????????g?????R2rams?d???e?????? 50ml?????????e?a?W?????????70?g3?nCJ0?g1?n??0?g15?n ??ICoA????f????????30ml?e????37Au???m?????? ???F2?d????3?d???g?-???????g?????F?????e???? ?b?????????e??2??105?d?????????????W?T10?nFBS?? DMEM?????????e?????g?????????0???????F?e?a?- ????????????370CCJ5?nCO?6???????????????e??3?m ?????e???????????e???s???C80??90?n???f?g?G???e?? ?U?????????????????S??Co ?a?W????2mgCJ4mg??????PGA?z?????R???? ?)?????????-?R?d?????g???0???????n?b?g???????1 ?I???????g??PBS???b????CJ75?n????????30?a?? ?????g????1501?g1DMEM+10?nFBS???????g??37Au ?s????48?d???-?l??????Co?r???9??CJ?????????? ?R????????P2(?U2??)?-?R?????73x103?d1501xl?? ?????????e?a?W???????E?e96??L???g?r??????150txl ???????????e?????r30???e????24?d???e???????x 2006??2?f?U2???U1?P ??Co?-?g?u?????????e??????10???????????74mg ???????e??10???????72mg?????????7???????6?? ?g10???0?H?(??DMEM???????????7???????e?? ?m????Co???????E2?A?e??????????t????Co 1?g2???????? ????2CJ4CJ6CJ8?m?g?e?r??L????5mg?dmLMTF???? 15pJ?e????????4?d??Co?-?g???????????e?(???????? ?????e????150pJDMSO?e???????????????????z?? ??????1?a???e??490nm?7???u?????????(?????3Co 2???? ?6??????????????????????OD?3?????????g?a ?????P???????????e?9?????????R?????????????? ?????????????H2?m???????E?g?8?g2?m?g???????? ?P???e?9???R???????????U5?m????(??1CJ2)CJ???? ?R???????????U6?m??????????(??3CJ4)?e???g???? ???????-?6?????R?????????????????????????0?H 24?d???g???????????P??(??5CJ6)?e???U3?m?????? ?C????Co?HExcel?????????n??????????????t?? ???e?T???????????????r?????????????????????? ?????g?8???o?ep>0?g05(??1)Co ??1?U2???9???R?????????e????????Co???(?????e?????????e?P?? 3?m???????????f?g?????c?6?D???c???b???? ??2?9???R???????????????????????????H2?m???????E?g?8?g2 ?m?g???????P???e???U5?m???????C?????e???g???????????- ??3?U2???????R?????????e???6????Co?????C?????(?E?d?e???? ?????????e?P??3?m?????????k???a???f?e???????c?6?D???c???b ??4?????R???????????????????????????H2?m???????E?g?8Co2 ?m?g?????????P??Co???U6?m???????C???? ??5?U2???????R?????????e???????e?????C???e?????????e?P??3 ?m???????????U???f?g???????b?????T?? ??6?????R???????????????????????????H24?d???g???????? ?P??Co???U3?m???????C???? JournalofTissueEngineeringandReconstructiveSurgery?eFebruary?g2006Vol2No1 3???? ?P???????z???????????s?F???????z???e?????? ?P???????????T???s?????6?P???????k??CJ??????CJ?? ?T???????????k????Co???????H?????R?????????7?? ?????????????????????????6?e????????(PLA)CJ???? ????(PGA)CJ?g??CJ????CJ??????????????CR1?e21CoPGA?z?? ?????b?6?2???k?H???????H?????????e???i??[3]???a ?????????????E??PGA???z???i?e???F????1???g ???E?????G???s?g6???g???????z?????c???????? ?b???????????6?????V[4]?????H?A???????????gPGA ?H???F?R???????R?????K?????6????[53???H?D?R???? ?????gPGA???R?E?f???E?????z?????7???????e?? ?????g???(?????H?k?????JCJ??????CJ???????b???? ?P???????k???????c?????3?????????s?F????Co?a PGA?gPIA?E?f?RPGLA?g?)???????n?E?v????CJ ?????????7???e???)??PGA?????????P????PLA?? ???7???????E?e?????R???f?R???T?E?e?????P???F ?????k????PLGA?V???????????7?e???????????7?? ?m?-???a?????????????[??PGA???7?z???e?g???? ???????f???F?????e?????R?7?????(???k???T?P?? ?5?k?????????s????Co?????gPGA?g?????????????? ???E?f?????(???e?z?????a?????P???z???????e?- ?e?e?????o?7?i?6???H????PGA?g???3?o?w?e?S???R ?U???e???-?????H???6?????????HCo?c???g?1???k?? ?f????????????PGA?H?a?F?k???i?g????PGA?? ???????e?R?U?S?k?k?????e?X???????e????????PGA ???P????????PGA?e?a?T?????????D?????6?F???[Co ?7?????P??????PGA?C?????O?????????e?? ?????e???????????????????????P???z?O???T???k?e ?????T???6?????e???X???????????Y?z?????????? ???????H???????????????????e?U???????HMTT?? ??[03?H???F??????????PGA?z?????????????T???R ???????????????????e???????????U??????Co???F?e ?????????z?O?o???????????e???????????z?O???T ???k???F?e???7???????z?s???????g???????????P ???????k?z?O?????e?H?g???????????????????X?c ???????e?T???R?????????H??PGA?i?????~CJ?(?? ???P?????????e?S???H?a??????Co?????e?c???S?-?? ??????PGA?z???????H?????R?????????7?????? ????????????Co ???u???, LiuWei?VCuiLei?6CaoYilin?gRecentadvancesintissue engineeringofcartilage?ebone?eandtendon?gCuurOpinin Orthopaedic?e2004?V15?6364-368?g ?????V?e???i???e?2?P?g?????????g???????s???z?S?????????e?P ???5?????s?g?x???g2002?V3?6166GS169?g CaoYL?eVacantiJP?eMaX?eeta1?gGenerationoftendonusing syntheticpolymerseededwithtenoevtes?gTransDlantation Proceeding?g1994?V26?63390GS3392?g ?????V?e???????e?2?P?6???g???F???????????s?F???????N???S???g ?X???F???[???g2004?V2?6110GS113?g ?????e?J?d???e?2?????e???g?????R???????????????????s?F???? ???N???S???g?X???5???[???e2002?V16?61105GS1107?g ???yCo?????E?e?Y?????e???gMTT???????5?H???a?????????????? ?k?g???1?P???5?????s?e2003?V1?646GS47?g (?-?4???P?62005??12?f27??) (?i???U17?M) ???????-??[4]Co?????k???????????????????????U ??Ca(NO?e)?6??(NH4)?6HPOCo?7????Co?n???????s?a?? ?????????T???????P???????C?????9???????X?e?? ???H?x?u????????pH?3Co?????FCJ?????b??CJ????CJ G2?????RBGSTCP???WCo?H?????X????Ca(N03)?6?????? ?P?????????????W??????????Co?+???c???6?????E ?eCa(NO?e)?6???????P???-?l?????????????e???C?f ?o??Ca(N03)?6?????P??Co?6???????????e??Ca(N03)?6 ???????P???a?W?7150ml?dh??200ml?dh?e?-?l?????? ?W??XRD???????7BGSTCP?????V??Ca(NO?e)?6???? ???P???????C300ml?dh???g?-?l?????W??XRD???? ?7HA?d[3??TCP?????f??Co?????????????P?????z?Y ???????P???D???e?????+????Ca2+?????????e?P?R?? ?????e?????E?????e???S?HpH?3?710???????eHA ???????????d?e???????i???j??[51Co?c???6???2?F Ca(NOCo)?6???????P???e??????????????????[3-TCP ???WCo ???u???, ???F???g?P???5?H?????????g?????6???????6?k???????8?g2000?g ???F???g?????g?g?P???????g???????6?k???????8?g27?g Jarcho~LBolenCI-1?gHydroxylapatitesynthesisandcharaeterization inDensePolyerystallineForm?gJMater?gSci?g1976?V11?620GS27?g ???????e???L???e???F???g?6??13??TCP?P?????????????-?l?? ???????g???????z?????e2001?64?616GS19?g ?2?e???e?????e???????e?????g?g?????????????????????????S ???g?F???????e1996?V1?610GS14?g f?-?4???P?62006??1?f9??1 l 2 3 4 5 6