人骨髓来源细胞外基质对人牙周膜干细胞增殖的影响

人骨髓来源细胞外基质对人牙周膜干细胞增殖的影响 王阿娴，唐 丽，梁 源，季海宁，武俊杰，丁 寅 解放军第四军医大学口腔医院正畸科，陕西省西安市 710032 Effects of human bone marrow cells-derived extracellular matrix on the proliferation of human periodontal ligament stem cells Wang A-xian, Tang Li, Liang Yuan, Ji Hai-ning, Wu Jun-jie, Ding Yin Department of Orthodontics, Hospital of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China 摘要 背景:研究发现人来源的骨髓细胞外基质能促进人牙周膜干细胞增殖并保持干细胞的特性。 目的:初步观察人骨髓来源的细胞外基质对人牙周膜干细胞增殖能力影响。 方法:分离人正常牙周膜细胞及颌骨骨髓细胞，制备骨髓细胞外基质膜片。采用有限稀释法克隆培养纯化 人牙周膜干细胞，透射电镜观察细胞超微结构。取P2代人牙周膜干细胞与骨髓细胞来源细胞外基质共同 培养，以普通培养基对照，采用CCK8法及细胞周期流式技术人骨髓细胞来源细胞外基质对人牙周 膜干细胞增殖能力的影响。 结果与结论:实验组人牙周膜干细胞较对照组相比细胞增殖能力显著增强(P < 0.05)，且细胞符合其生 物学形态及生物学特性，生长状态良好。说明实验建立起的骨髓细胞外基质能够促进牙周膜干细胞的增殖， 是一种扩增干细胞的有效方法。 中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚 胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程 全文链接: 关键词 : 干细胞; 培养; 骨髓细胞; 细胞外基质; 牙周膜干细胞; 增殖; 国家自然科学基金; Abstract: BACKGROUND: Human bone marrow cells-derived extracellular matrix can promote proliferation of human periodontal ligament stem cells and maintain stem cell properties. OBJECTIVE: To preliminarily investigate the effect of human bone marrow cells-derived extracellular matrix on the proliferation of human periodontal ligament stem cells. METHODS: Human periodontal ligament stem cells and bone marrow cells were separately derived from human periodontal tissue and jaw bone marrow, and human bone marrow cells-derived extracellular matrix was prepared. Human periodontal ligament stem cells were cultured and purified using limited dilution cloning method, and transmission electron microscope was used for ultrastructure observation. Human periodontal ligament stem cells at passage 2 were cultured with human bone marrow cells-derived extracellular matrix and normal culture medium (control group). The cell counting kit-8 and flow cytometry were used to determine the proliferation potential of human periodontal ligament stem cells cultured on human bone marrow cells-derived extracellular matrix. RESULTS AND CONCLUSION: Compared with the control group, human periodontal ligament stem cells cultured on human bone marrow cells-derived extracellular matrix had a superior capacity of proliferation (P < 0.05), and the cells met their morphological and biological characteristics, and grew in good conditions. Human bone marrow cells-derived extracellular matrix is a promising matrix for large-scale expanding human periodontal ligament stem cells for future use in stem cell-based therapy. 中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程 全文链接: Key words: stem cells; extracellular matrix; periodontal ligament; 中图分类号: R394.2 基金资助: 2011年国家自然科学基金青年科学基金项目(81100750) 通讯作者: 丁寅，博士，教授，解放军第四军医大学口腔医院正畸科，陕西省西安市 710032 武俊杰，博士，讲师，解放军第四军医大学口腔医院正畸科，陕西省西安市 710032 作者简介: 王阿娴，女，1987年生，陕西省汉中市人，汉族，解放军第四军医大学在读硕士，医师，主要从事口腔正畸临床及细胞外基质基础的研究。 引用本文: 王阿娴,唐 丽,梁 源等. 人骨髓来源细胞外基质对人牙周膜干细胞增殖的影响[J]. 中国组织工程研究, 2014, 18(6): 938-943.