【doc】REM睡眠剥夺后大鼠脑组织氨基酸类神经递质含量的变化

【doc】REM睡眠剥夺后大鼠脑组织氨基酸类神经递质含量的变化 REM睡眠剥夺后大鼠脑组织氨基酸类神经 递质含量的变化 2062???/16V??/3?A ??8Q??V;???? REM???B%??F?p?q?B?i?????u?? ?????????v?u?m????V?Vn ?8?"????V*?? ????????:???????B%??F(sleepdeprivation,'SD)???q?B?i????Y-?u??82??(GABA)52?k?u??(GLU)?m?????????l?? ??:?u?d??V??Q??????(flowerpentechnique)???q?B???>????96h???A%??J??(rapideyemovernent,REM)???B%??F?l8????z?? [???V??q??V???8F?????l???_???????N???}?????B%??F?p?q?B??V????u52?i??52V?8'?i?@?iVFGABA,GLU?m????V?Vn ????:???B%??F?p?q?B?1?iVF?i????GABA?m??,GABA/GLU!J?*?????????p?]????(P<0.01)?l?lGLU?mhi?u?i??528s 8'?i8w?iVF8??????????p?]????(P<0.05)50????:???B%??F?????q?B?i?????u???????????v?u?m??V???=????TV??l?. ???B%??F???s???????A?????F%g8}8?50 [????????B%??F?k?u??Y-?u??82?? EffectsofREMSleepDeprivationonAmino8?acidNetu-o?zransmitterContentsinRatBrain LIQttt8?.hi,WANG-9-g8?sn.IkpzrzmentofTrudititmalChineseMedicine,FirstMilitaryMedsnlU;izvaity,Geuung 8?Jest510515 5rAbstract]ObjectiveToinvestigatetheeffectsofREMsleepdeprivationonGABAandGLUcontentsintheratbrain.Methods Acinvilsmererandotnlydividedintothreegroups:REMsleepdeprivationgroup(small?Ntf orms6.5crnindiametcr,SD),tatdccontrol group(Iargcplrafotn50??48??l50diameter,"IC)andcagecontrolgmup(C(?iTheratsofSL)group8?8?deprivedofREMsleepfor96 hoursbyflowerpottechnique.Highperfornnanceliquidchmtnatography(HYLC)wasusedtodeterminethecontentsofGABAandGLU inthreebrainregionsoftherats:frontalcortex,hypothalamusandbrainstem.ResultsSDgroupshowedtheincreaseincontentsofGA BAaswellasratioofGABA/GLUinallthethreebrainreg?M50ns(?z<0.0l)compared%?CCgentq5oandGLUcontentswerealso??e ntcrutsedinthebrainstemandhypothalamusofSDgroup(P<0.05).InsignificantchangesinGABAandGLUcontentsofthethree brainng8?8?werefoundinTCgroup.ConclusionREMsleepdeprivationinducessignificantchangesinthecontentsandproportionof themnint)8?acidneurotransmittersintheatbrain 5rKeywads)REMSleepDeprivation;GLU;GABA;Neurotransoutter;Rats 8P??%R??V?to42???<?E???2A ???B%??F(sleepdeprivation,SD)?.?E?F=V???????3?2?????qSpragueDawley?q?B350V?(?/?L???? ?????l??%R?n[???8q?N???z???B???????250??????%???8P????=?),6-7?????l=V??(190110)g,???F%R ?????B%??FV???8P??????????%???8??A????????8F???z??[???(cagecontrolgroup,CC)52?q??V??????? ??B'?????B%??F???t????8???8???8????i???????>((tankcontrolgroup,TC)?????B%??F??(rapideyemove- 8F???RV??E?????B???????C??[??:?????d8@?l?l??8?8?ntsleepdeprivationgroup,SD)????10V?50????%Z%R ?.[?8?V;??52?y??52?y???@????V;???t????????8}8?50?J??[??0??????1???l???????s8P%???V????N?????? ???:?t???l5o?????l?D???????v?uV?8p???B8p???????A?Q?l???9=??2(25?A2)Y'?l[@?????A12/12h ?R?6?????s?l=7[???%E??=??u?F%g?w[??.?u????????1.28???????%" ???v?u?u???B8e?l????8PV????B8q?N???28s??V?Vn???cLC-6A?????_???????N8?(? ???%R???}?? 8??y??8???[??r?p50?/?????D?????q?B???A%??J??*.5252525052?l50?l528?525050?l 501?l?l52*8??l508?8?c___ (rapideyemovementREM)B@&K34A496hif}}.}??}i>i1llll}}r}frfo?????v?u? m????V?Vn?l?????u???????????v?u8p???B*-Z?<:31???V???u?d??V??Q??????(fl owerIx)t ?!???????? 5t????51051U) 18?????%???V?%R?? ?g?O9?A:20028?048?03 =??3V?=Z?/8?[?Ik?q??8PV;???o????=w???t?? ??=????M:[?[?B'8?8?V;?????t??%v?x%?????(?nV?:96M(KJ) 2002???/16V??/3?A techniyu???l??%g?B???e%g???B%??F%????L??50[?8P SD??V?'1Y'????V?????%R%m8F4.5cm??16cm50%9?u ??????V???SD??????%??????>??????REM???B%? ?F,75t??%?????V????????z???B?n?l[???????8pSD ????[????l 1.4?i?????????v?u??=??? ??SD?????>????96h?????B%??F?p?l?j?:%??? 8??l8????![?(9:00-10:00am)???Q?1???l[???8(?? ?A%R??V?=???V????u52?i??V?8s8'?i??=Z?i?????l?W ?!?P????V??????p?l=8?9[???(8?%?L6)=????E?u50?R [?[??i?????]8?????V??7??8P?l%?8??C??3nil?t???l ???????1?R?????????lV??7??[?[????A(4'C,1000??/ tmn)????30min?lV?8(?3???Q???????C???l%????Q8??? 5rnl?l[??L[?[????A????30min?lV?8(?3?l???_???????N 8????}GLUV?GABA?m??(1uM1.???i??)50 1.5???????1?R???? ????????????%9?uSPSS10.0forwindomB'??8??? ?>?D???k?/?*??????%R??(one8?wayANOUA)?1?R50 2.3?q?B?1?iVFGABA?m?? SD??%?????V????u52?i??528s8'?i8:8y?iVFGA BA?m???*???p?]?????l8pGC?????????0?]??(P< 0.01)?l[?8P?w8???V????uV??i??VF???????K???q((2 !e8?8()?l?lTC??8pGC?????????]?????0(P> 0.05)0(????2) ??2?q?B?i????GAB[??m??V???52) ??V????u8s8'?i ????1.38?A0.32 [j??1.4?l8(0.29 SD??4.55??0.79" 1.4410.44 1.40-0.48 3.4810.80.' 4.16?A0.52 4.45??0.69 7.61=1?3???} ??:8pCc??????BSKP<0.01 2???? 2.1????%???8??/??[? 8pTC,CC???????lSD??%????u???????A?lV??? V?24-48h???B%??F?A???l????8F8?????[??}?q?> 8F?lV??;%????D?l???n?>??52[@?@%F???=?A;?l?u???? 8P?p?A(72h???B%??F??????????%Z),SD??%????>8F ?N[??}?r?r??8F??%g?l????8F????8e?.?l???z=8?Q?l?? ?V?u?lV??e???b?Q8P?l?????p?eV??/8(??V??l?;%?[? ?i?l???n?>%F??V???[??NaIL6??8p(L6?????????p?] ???050 2.2?q?B?1?iVFGLU?m?? 8pGC???????lSD????V????uGLU?m?????p?] V?Vn(P>0.05?o?l?i??528s8'?i8w?iVFGLU?m???p?] V*???l??????%R?????0?]??(%R%m8FP<0.05V?P< 0.01);?lTY:??8??u8s8'?iVF?]?F[?GLU??????%? ((P<O}05)?l[????iVF8pa!?????????]?????050 (????1) 2.4?q?B?1?i8'GLU/GABA??!J ????%????1?i8'GLU/GABA??!JV?Vn??[?8p GABAV?Vn?????>?lV?SD???1?i8'GLU/GABA??!J ?*??CC???p?]?Z=8?l??????%R?????0?]??(P< 0.01)?l?lTC??8pCC?????????]?????0(P>0.05)a (????3) ??3?q?B?1?iVFGLU/GABA!J8??A52) ??V????u?i??8s8'?i ????7.8511.40 8?[???7.8512.33 V???2.36?A0.19' 3.45?A0.49 3.53??0.66 1.70??0.41'BY 2.06i0.21 2.17-0.15 1.54?A0.10'?l ??:8p(c??????8?P<0.01 3???? ??1?q?B?i????GLU?m???4??:) ??V????u?i??8s8'?i CC?? TC?? ST?? 10.38??1-24 11.06'_1.61 10.64??t.24 4.81??18? 4.67??0.84 5.68?A0.93" 8.5311.03 9.57??1.06" 11?Q?A1.19"BY ??:8pCC??????B'P<0.05;"BSP<0.01 ???B8p?????j??V????????F%g???z?e?"?l?D???? ???v?uV?8p???B8p?????A?R???A???6??50?k?u?? (GLU)??y-?u??82??(GABA)?.??8?%???8P?????? ????[??????????u???????????v?u?l??????[H???;%? V???[?????%R%m[??:[??}?qV???%g?q?6??=??u50?: ?t??[[as5e???F?l8e?l?iVFGABA,GLU?m??V?[?V?=V%? ?????TV?V?8p???B???????sV?8e?l???B???????????l ?u???B???6??8PV??c?j??????=??u?l??:???B???28s ?i????GABA?m?????3????V*??15%}el;8s8'?i??%Z VFUABA??????[H??V??q?]?WV?GABA8?AV?=V?? ?;V?8p8s8'?i?????B?w[??.???A%??B??(rapideye movement,REM)???B???6??ill ???B%??F(sleepdeprivation,SD)?.?E?F=V?????? 2002???/16V??/3?A ?????l??%R?n[???8q?N???z???B???????250SD???F =V?A?R%????????????l?w8???8P?????????????????? 8F?p?];Si50??????SD?p8P???????????w[??.?q?i?? ?"??%????18????Z????%g???2?l[??F%gV???8p??8??? ???v?u???TV??:[?[[6150?q?i???"?.?1???A??=?V??? 8????M8P???l???"????[H[??}?q[????F=V???????d?? ???2;?i??????V3?m?:8(?>?[?????;?????l[?GABA ??????[H?;?q8pREM???B[?????%E;8s8'?i?D???? ????[?%R???[?????6???l???2[??????I???l??V?8p?? ?B52?x??52????V????@?D???A?R%??????6??50??8:8y ??=Z????[H??[??}???2????????%??????B???????s ??????50 ?/?????????]?F?l%???????96h???A%??J?????B %??F(rapideyemovementsleepdeprivationREMSU)?p?l ?????>8F????8F?N[??}%5??%g??V?Vn???s?l?i????8P GLU,GABA8w???????v?u???m??V?8??3????!J8?V? ?A8??]??V?Vn50[?8PGA13t52?m??52GABA/GLU??!J ?u?j????1??8:8y?iVF(??V????u52?i??528s8'?i)?*?p ?]?????l?lGLU?m??8??u?i??528s8'?i8w?iVF?]?F[? ??????%??l?u??V????u%SV?Vn8e?p?]50V??A????V?Vn ??V???V???V3??:w?SD???s8P?F=V?????????2?3?? ???2?l=V%??q?????,?l8P??52?n??8??U8??????R????[? ???????(8??????u???????????v?u8??U???TV?;w??F =V?????B8q?N??8?8e??????%F?????????qV???50?? ??8?[?%R????????%???V??A8?!??q???0???q?TV??l?D ??8s8'?i8???=V8???8(??????V????q?6??=??u?l?TV? 8P???????v?u??%??????2?l=??F=V?>8F8p?j?1???n?> ???????0??50w?????[H???2??[??}???2????[??}?q ?????v?u??????%9?u?????,?l??%g?q?v?u%SV??A???? ??????!??????R??508?8(??8????B??V?????8P???? ???v?u?M?r52???]52?xV?52?Z?????s???TV??l8;???.?? %g?q?u?????v?u???M?r8p???]??%?52?xV?52?Z??[??i?l ??????%g?_??V?='%??l=?8P??????????%????18????? ????%g???2?l8??C??????[H???????????2[??}???2?l ??????%??l????[??}?q?v?u?????????q=??u??????[H ????=w?l8??lV??cBY=????q??%gB-=??u50 ?i????8PGLU?m???????l=7?q??%R??8??U8P?? 8????l8??:???i??%R?>=??????v?u??%????l[??m??V? ?F=V8??U?Q???????????q?l?K8e??V???8?GLU?m?? ???D?i??%w??????%?????[??}?n??%g???2;GA13A8e ???d???j?i?=???l?i????8P??GABA?*?NGLU???k ?u???N?????N???l?A?r?l8w?38??U???????TV?V????? GLU/GAGA??=???V?Vn?l????!J?z?u=??t??????8? 8P??????????%???[??}????%g???2???????E?E50?/ ????8PGLU?m???uSD?q?B??8w8y?iVFV??q??V??? ??????8s8'?iVF?_??V*????%??l?6?[8p???????????? ?F=V8??U???2???TV??:[??l?l?l???j=Q???????iVF GABAV?GABA/GLU??!J?q?K????V*???l8?=????? %???????%g???2V?='%?50 ?d??V??Q???????B?L??????%??????????? REMSI5o?n?l?D?:????????52?;%??!%g52=V%???%?52V??e ?b?Q?@?D?????????B???u9]?l???D??%F?????B[??l=? ?u?l??%?????????8?????8F?D?????l=V[?V?Vn???A?R ?F%g???z?e?"?l8P???q??%??.8;??????50?q?i%????? ???Z??%g???2????8?8P???q(????)??%???V??A?l?o?? ??%??A????=B8?V??1?????A?R52???R?F????8s?Z50?l ???l????=????q??%g=??uV?=??F=V[??i???,?l?w[??. =?8P??????????%???[Z8??????A???2???????2??[? ?}???2?l??V?8eV??R??????50?/???????????F?l RFA4ST)?????i????8e?l?iVFGLU,GABA?@?u???? ???????v?u?m??V?5tGLU/GABA??!J??V?Vn?l??V?Vn V????.SD??8P??????????%???8s?Z???????A?????F %g8}8?50 V??6???< 1ForestG?lG5o8DboutR.Effectsofsleepdeprivationonpetformanc, andEEC'spectralanalysisinyoungadults.BrainCogn,2000,43 (18?3):195--200 2KruegerJM,Oha?M?lKapasLeta!.Brainorganvationandsleep function.BrainRea,1995,69(2):1778?185 3MendelsonWB,GuthrieRD,FrederickGetal.Theflowerpot techniqueofrapideye.(REM)sleepdeprivation.fhar- macdBiochemBehav,1974,24(2):553-556 4IceRS,SteffensenSC,HenriksenSJDlsehargeprofilesofventral tegrnmtalareaGAHAneuronsduringmovement,anes??hesia,and theslap8?52vakecycle.JNeurcsci,2001,21(5):1757-1766 5MardridiA,B-billsD,ManciaM.Sleepisdifferentlynodular edbybasalforebrainGABA(A)andGABA(B)receptors.Am) PhysiolRegalIntegrCompPhysiol,2001,281(1):81708?5 6YoyamsY,KayacaAY.Propertiesofneuronsparticipatinginrcg- ulationofsleepandwakefuness.AdvancesinNenrolcgicalSa 8?.1995,39(1):14-15 7??M,IhaSK,Kaur5.RoleofGABA8?Areceptorinthep-p- ticaream??eregulationofsleep8?wakefulnessandrapideye nnvementsleep.NeurcscRea,1999,33(3):2458?250 8ThomasM,SingH,BdenkyGetu1.Neuralbasisofalertness andmgriitivcperformanceimpairmentsduringsleepiness.1.Effects of24hofsleepdeprivationonwallinghumanregionalbrainattivi- ty.JSleepRrs,2000,9(4):335-352 9PokkP,VassdjevV,ValiM.Smallplatformstressincrease; FundamClinPhamtacol,200(?l14(5):4878?491 (8P???n????V8) (?????n???Q=!??)