单侧输尿管部分梗阻论文：单侧输尿管部分梗阻后肾盂平滑肌功能及其超微结构改变的研究

单侧输尿管部分梗阻论文：单侧输尿管部分梗阻后肾盂平滑肌功能及其超微结构改变的研究 单侧输尿管部分梗阻论文:单侧输尿管部分梗阻后肾盂平滑肌功能及其超微结构改变的研究 【中文摘要】对单侧输尿管部分梗阻后大鼠肾盂平滑肌功能及其线粒体超微结构的改变进行测定。方法:取80只健康雄性Wistar大鼠，体重200?20g，随机分为8周实验组、16周实验组、8周对照组、16周对照组，每组20只。对照组仅分离左侧输尿管，将实验组大鼠左侧输尿管上1/2段分离后，包埋入同侧腰大肌，建立PUUO模型。造模完成后，分别于第8周和第16周分离取出肾盂，测定肾盂平滑肌的收缩频率和收缩力，将肾盂组织固定、包埋、切片、脱水、封片干燥后，置于透射电子显微镜下，对肾盂平滑肌细胞中线粒体结构的改变进行观察。结果:(1)8周实验组肾盂平滑肌收缩频率和收缩力分别为9.68?3.24次/30秒，0.45?0.13g;8周对照组肾盂平滑肌收缩频率和收缩力分别为8.47?2.19次/30秒，0.24?0.10g。16周实验组肾盂平滑肌收缩频率和收缩力分别为7.32?1.56次/30秒，0.13?0.05g;16周对照组肾盂平滑肌收缩频率和收缩力分别为次8.29?2.26次/30秒，0.22?0.09g.(2)实验结果明，8周时，实验组肾盂平滑肌收缩频率、收缩力均高于同期对照组(P<0.05);16周时，实验组肾盂平滑肌收缩频率、收缩力均低于同期对照组(P<0.05);对照组在8周与16周相比，收缩频率、收缩力的差异无统计学意义(P>0.05);8周实验组与16周实验组收缩频率、收缩力 的差异有统计学意义(P<0.05)，8周高于16周。(3)单侧输尿管部 分梗阻早期肾盂平滑肌细胞线粒体数量明显增多，结构基本完整，但 梗阻晚期线粒体肿胀、空泡化，数量减少。结论:在输尿管部分梗阻 早期，肾盂平滑肌收缩功能增强，处于代偿表现;在输尿管部分梗阻 晚期，肾盂平滑肌收缩功能降低，处于失代偿表现。 【英文摘要】:To observe the changes of the function of renal pelvis smooth muscle andultra microstructure of mitochondria in partial unilateral ureter obstruction.Methods:Eighty male Wistar rats were randomly divided into two groups: shamcontrol group (only received laparotomy)and experiment group (with the upper half of ureterembedded into the psoas muscles). At different time points (8-week and 16-week)，the renalpelvises were caught out, then the contraction frequency and extent of the muscle strips weredetermined. And the changes of ultra microstructure of mitochondria in the renal pelvis smoothmuscle cell were observed by transmission electron microscope.Result:The contraction frequency and contractile force of smooth muscle in renalpelvis were 9.68?3.24 times / 30 seconds and 0.45?0.13g in 8-week experimental group, and8.47?2.19 times / 30 seconds and 0.24?0.10g in the 8-week sham control group.They were7.32?1.56 times / 30 seconds and 0.13?0.05g in 16-week experimental group,and 8.29?2.26 times / 30 seconds and 0.22?0.09g in the 16-week sham control group.Compare with thesham control group, the contraction frequency and contractile force of smooth muscle fromrenal pelvis in experimental group were increased at 8-week time point(P<0.05), and decreasedat 16-week time point(P<0.05). They were no statistic changes between 8-week and 16-weeksham control groups(P>0.05), while they were significantly higher in 8-week than 16-weekexperiment group(P<0.05). Mitochondria in the renal pelvis smooth muscle cells at 8-weektime point were significantly increased,but microstructure were hardly normal. Mitochondria inthe renal pelvis smooth muscle cells at 16-week time point were decreased, the ridgedisappeared, swelling and empty bubbled,and a large number of collagen fibers appearanced inintercellular substance.Conclusion:Partial unilateral ureter obstruction can enhance the contraction fuction ofsmooth muscle from renal pelvis at 8-week time point. However, the contraction fuction ofsmooth muscle in renal pelvis are decreased at 16-week time point. 【关键词】单侧输尿管部分梗阻 肾盂平滑肌 收缩力 收缩频率 【英文关键词】Key word partial unilateral ureteral obstruction renal pelvis smooth muscle contractile force contraction frequency 【目录】单侧输尿管部分梗阻后肾盂平滑肌功能及其超微结构改 变的研究 摘要 5-6 Abstract 6 英文名称缩略词 7-8 前言 8-10 材料和方法 10-14 结果 14-15 讨论 15-17 结论 17-18 参考文献 18-19 附录 19-21 综述 21-26 参考文献 24-26 个人简介 26-27 致谢 27