非公平竞争对竞争抑制法检测抗-HBc总抗体的影响

非公平竞争对竞争抑制法抗-HBc总抗体的影响 非公平竞争对竞争抑制法检测抗-HBc总抗 体的影响 [?????o??]1002-0179(2008)05-1103-02 ???????????????????R????????-HBc?w?????????? EffectsoftheUnfairCompetitionontheDetectionofAnti-HBcbyCompetitiveInhibitionMethod ???o??,?????? PENGShi-Guo,HUDing-Yuan (?????[?S?????E?w??,?????c??6?610016) (No.4SichuanPeopleDnsHospital,ChengDu610016,China) 6?6?????:????:?t???????????O?J????(ELISA)?,????Dr?v????DC?u?????O???w????(??HBc)?w?????.?? ?????7?S?H???????7?????,?????8?3?5?????c??Dr?3????DC????????????????6]????:?R????????HBc?l?d?7 ?S?????-?:?d?7?S??90??,?7?S?????????????V???3?5?????H????????HBc-???j???d?o????(HRP)???-?7?S ?H??HBc-HRP?????????H??????????????,?%???????H?p?????1??????????,???5???1???.???????7?S?H???? ???7?????,?????8?3?5????????????????(HBsAg)?l?d?7?S?u??-HBc??????6]????:??????HBc?l?d?7?S?? ???7?S?H???H??????HBc-HRP???????????????F?l?d???a,?X??????????HBsAg?l?d?7?S???????B6]????:?? ???7?S?????????????H????????HBc-HRP?5?s??????HBc?F?l?d,???a???,???????????????7?S????HBc- HRP??????6]?5???????F?l?d?,HBsAg?l?d?7?S?u???S?3???,6] ???g??:?????????O?J????;?????????????O??????;??????????;?F?l?d [?u???-????]R4461626?6?6?[???g?7???[]A 6?6?Abstract:Objective:Toinvestigatetheeffectsoftheunfaircompetitiononthedetectionofanti-HBcbycompetitiveinhibi2 tionmethod.Methods:Ninetysampleswithpositiveandnegativeofanti-HBcweredividedintotwogroups,namelyexperiment groupandcontrolgroup.Theanti-HBcwasdetectedbyELISA.Incontrolgroup,totalanti-HBcHRPwereaddedfollowing addingallofsamples.Inexperimentgroup,thespecimenwasaddedafterblendingofsamplesandanti-HBcHRP.Andthen theresultsfromtwogroupswerecompared.Meanwhile,theeffectsofdifferenttimeofaddingHRPonanti-HBcfrompositive sampleswereassessed.Results:Incontrolgroup,sampleswithfalsepositiveofanti-HBcweresignificantlyhigherthanthosein experimentgroup.Meanwhile,thelongerthetimebetweenaddingofsamplesandanti-HBcHRPwas,th ehigherthefalseposi2 tiveratewas.However,theeffectswasnotpredominantinHBsAg-positivepatients.Conclusion:Theant i-HBcfalsepositive rateleadingbyaddinganti-HBcHRPfollowingaddingallofsamplescanbecontrolledbysimultaneousa ddingofanti-HBcHRP andsamples.Atthesametime,thereisnofalsepositiverateinHBsAg-positivesamples. Keywords:ELISA;anti-HBc;unfaircompetition;falsepositive (Receiveddate:2008-01-14) 6????2???????o??(63),?;,?? ?????]??,???w?????9,?w??????6] 6?6??????????O?J????(ELISA)?Y?? ???:???8????????????????(HBV)?% ?2?7?????????7?7?????????O?,???? ?? [1] 6]???????7?????u?????????????? ??(HBsAg)6[????????(??HBs)??e ????(HBeAg)???,???Y???????j?? ??,?5?H?v??e????(??HBe)6[?O?? ????(??HBc)?w?????n???,???????R ??6]?E???,?????????????u?????????Y ??HBc?????l?d???????2???6?????w ???3?????O?????????3??????????6]?T ??,?E?????????p?-?????T???????Y?, ???O?????u?w?Y???c?N?????7?S?H???? ????????HBc-???j???d?o???? (HRP),???????S???N?7?S?6???????H ???N?7?S,???u?????????????E?n 10min,?a?n30min?a??????(?????? ?5???????????7?S?????????7?)??), ???;?.?D?c?????????7?S?u?????????? HBc?o?????]?d???,?U???H?????8???O ??????(HBcAg)?????9,?H?H?????? ????HBc-HRP?H?V?,???????H?8???c ??Dr??????DC????,?t???;?H???????? ?7????????????????,?F?l?d???a [2] 6] ????Dr??????DC???????????????????? ???(?a?d,?T???E?????????)???????? ????Dr??????DC???????H?V???,?????6 ?????e??????????6] 6??????H???? 1116??7?S?R???????7 ?,?E???????????u?R???????????? HBc?l?d?7?S?????-?:?d?7?S??45???? HBsAg?l?d?7?S45????90???7?S,?( ???/?????/??1bR30?????H?7?,6] 1126????/?????? ???/???,?8???????p???(?????? Dr?v????DC???????/??,???p?M?9???/, ?.WELLSCANMK3???7??????????, ?(???/???????%???????:?d???????O?? ???x(A450nm)50%?T?:?l?d?v???? ?:???x,?7?SA450nm?O?????x?d???: ???x?T?:?d,?B???:???x?T?l?d6] 1136??p?????1?????????? ?(??Dr111DC???7?????????7?S, ??45?Y?2?[?B????,???Y?????????? ?N?7?S,?(???/?????H?????/??501?L ?7?S???????u(?K???3?????????????? ???7?S?????.),???????????????? HBc-HRP?????7????,?v?2???9?H?? ????????????????????,???;?7?S?u?? ??-HBc?????????7?????u????-HBc -HRP??????????????HBcAg?,???? ???H?8?.?Y??????????6] 1146??????-?? ?S????:?-???7?????????7?S???, ?7?S????HBc-HRP?(Dr113DC?????? ?,?????????????9?H????????????,?? ???n?-?G?,?????-?????7?S?????????? ?H56[6[??3????????B R,?(???/?????/?7???????H?V3A1 ?:??3,?h??,?V??,?v??????, ???????,???????????7?S?H?V???3?5?? ??????????HBc-HRP?????????????? ??6] ?S????:?-???7?????????7?S?H?? HBc-HRP?,??????????????,?????? ????????,?-?G?,37A1????106[206[ 30min?H?h??,?V??,?v??????,???? ???,?????????7?????H?????3?5?????? ??????????6] ?S?(??:??45???,ELISA?????? ?????5????????HBsAg?l?d?7?S45??, ?-?;?S??1bR30?????H?????????????? ?5?S????,???????,???????????7?S?H ?V???3?5????????????HBc-HRP???p ??HBV?p???2??????????????6] 26????? 2116??????7?S?V???3?5???????????? ????(?S???????S?(??) ??????????,?????7?S?????????? ????5min?H????????HBc-HRP???( 3?N?F?l?d,????30min?H???????? HBc-HRP?(14?N?F?l?d(P<01005), ????1?u?????)?????%?????????????? ?3??????,?F?l?d???????,?3??????, ?5?t?????l?d??????A450nm?O?????x ?,?3???6????,???V?T?l?d?????????? ???5?c?c???T6] 1( ?S????) ?????u?????)?????????H?????? ???????????3?5??????????????,???? ?,3?a???????l?d????6]?0?7?S?, 3011?????w??2008,23(5)6?CN51-1356/R ?? :19- 110200minHc- HP7 0min 22 2 0min 11( min) ?:?d???l?d?? ?F?l ?d?? ?S?(?? ?V?????? (min) ?l?d???:?d?? ?F?l ?d?? ??????37806???????4140 534113811%6?54140 10311461412%6?104231215% 20281792413%6?204231215% 302322143718%6?304322510% A450nm?0???O?????x???G?,10%??, ???????7?S????HBc-HRP????10min ??30min???????????H?V????(P> 0175)6] 2136??????7?S?H?3?5???????????? HBc-HRP??HBsAg?l?d?2?%?2??HBc ??????????(?S?(??) ?????????H???????7?S?V??56[106[ 20??30min?H????????HBc-HRP,?? ???V?T?F?l?d???????d?d???E,???a?? ?(2??,?????HDr??????DC???????p?? ???????????T?M?2??HBc???????????? ?B(P>01257)6] 36????? ELISA?????%?2?u??HBc?w?????? ???-?Y???,???????)??????????HBcAg ?2????????????,?????????H???%?2?7 ?S???5????????HBc-HRP,???????? ?7?S?u????HBc?????7??????????HBc -HRP???????9?????8??HBcAg6] ?:?????????????7?S???S?8?????, ???????;,?????,?7?S???0?????????? ?7?????????)???c93?N?????7?????? ???????????;?????,30min???8,???7 ?S?N???????7???n?%?,??????????6]?? ???R???,?????????7?S?u??HBc?U???? ???H?????8??HBcAg???9,?j???????? ?-?????????????9?(???p??????,???; ??????????HBc???????????d???a???? ?????????9????,?H?H??????????HBc -HRP???c???H?V??Dr??????DC????6] ?.??????HBcAg?????????????????H?% ?2?u???????????]?d???,?o?2???x???? ???,?(?????]?d?????o?2???????? [2] , ?X?????????,???????????-???A?B?)?H ?????8HBcAg?????9???????????]?d?? ??????HBc-HRP,?????????T?-???? ?:?d,?R?5???z?????Y?,????Dr?????? ????DC???????A?B?)????,?????,1bR30 ?????)?S???3?????????????o?????]?d ???,?U????????B???9??,???5?D ?c????,???p???d?????F?l?d6] 6?6????,?????-?7?S????BR?? ??211 037800 2037800 303691217% ???H???????????u,?????????.???S?8 ???????.?????????H?3?????5?????? Dr??????????DC,???5???????,?-?????? ???????????A?B?)????,?E?????????? ???)?????????T?p???7?S????6]???S?? ???u?????)??,?,?????;?S?H5min6[ 10min?H????????HBc-HRP?.?a???p 1612%???F?l?d,30min?F?l?d???? 40%6]?X?S?(???????????D?????????? ????:Dr??????????DC???p?????????? ?p???2???????(?d?????]?d??HBc???7 ?S???S?8???(?N?d????6] ?.????HBc?w?????2????HBcIgM6[ IgG??,??-HBc?w?????l?d???Y???? ?p?????7??,?????Y?I?dHBV?p???? ???p [3] ,?????F?l?d?????v???Y??HBc ?F?l?d?F?a???????2?D?c?3???(?????? ?p?????????p??,?????:???????????? ??6[??????????????,?a???s???w???? ??,?v???Y?????????u???????????? ??6]???a????????HBc?l?d?????w???? ???2???????????????2?p?6???? [4,5] 6] ??????????????HBc???,?????????R ??,?????????,???p???3?e?j??,?E?? ???T???,?-?7?S?H??BR?????? ???H??????????????,???;?.???j?S?8 ??????Dr??????????DC,???????,???? ???????-???A?B?)????6]?????????Y: ?????????????[???????B????,?????? ??????????HBc-HRP,???B???K???? ?6?????????????E???????J?%???.,?? ?,?????????????????????/?????H???8 ?????????????7?S,???w?-?????????7 ?S-???7???????9???????????????? ??,?????H?????????????O???????? ??,?????????????e??,???t???(???? ????Dr??????????DC???????F?l?d?? ??6] ?w??,ELISA??????HBc???????Y ???T????,?????K???????????u???F?l ?d????,??????????????,?5?3?????? ???????????l?d?????j?T?:?d [6] 6] 46????1???g: [1]???a??,???3,?I????.ELISA???H MEIA?????????????????7?7?????-?? [J].?????w??,2003,18(2):238. [2]??????,??????,???l.???;?????? ELISA???????R????????????????[J]. ???,?w???/??,2007,14(15):2012. [3]???K?K,???l.?:???????????%?2?A?B ????[J].?????w??,2005,16:62- 63. [4]??????,??????.???????????????%?2 ?7?????????????a[J].?????????w??, 2002,20(2):119. [5]?U??,?:??.?????????????????????% ?2?7?????????a[J].?????w??,2000, 11(9):545. [6]??????,???K??,?????y,???(???? ???????????????O??????????????[] ???o?w???:???p???o???H?????-??, 5,6(5)3 (?R?W???p) [?????o??]1002-0179(2008)05-1104-02 ?x?????[???0???u?????????D???????????t?? ApplicationofBacterialEndotoxinTesttoNizatidineInjection ?????? 1 ,?U???? 2 ,?????? 1 WENXiao-ling,LIUXing-lan,RENXi-ru (11,???????z6?637000;21,?????c??6?610036) (11NanchongCityInstituteforFoodandDrugControlofSichuanProvince,NanchongSichuan637000; 21SichuanProvincialInstituteforFoodandDrugControl,Chengdu610036,China) 6?6?????:????:?????x?????[???0???u?????????D??????????6]????:?,?3?5???????????/???x????? [???0?? ?-?G????????????6]????:?x?????[???0???H?????/??????????6]????:?,?????????D???????x????? [???0???u ???????????D?Y??????6] ???g??:?x?????[???0??;?????????D;?????/ [?u???-????]R286106?6?6?[???g?7???[]A 6????2????:??????(1965-),??,?? ????????,?w?????9,?-??????6] 4011WESTCHINAMEDICALJOURNAL2008,Vol123,No156?CN51-1356/R ?? HcAg Hc-HP Hc-HP . J. 2002:20. :2008-01-14