瓶子草组培快繁和植株再生（Tissue culture, rapid propagation and Plantlet Regeneration of bottle grass）

瓶子草组培快繁和植株再生（Tissue culture, rapid propagation and Plantlet Regeneration of bottle grass） 瓶子草组培快繁和植株再生(Tissue culture, rapid propagation and Plantlet Regeneration of bottle grass) Tissue culture, rapid propagation and Plantlet Regeneration of bottle grass [author] Hu Zijuan [degree type] master [awarding unit] South China Agricultural University, [tutor] Yang Yuesheng [year] 2009. [pages] Abstract: bottle grass is a newly emerging ornamental insectivorous plant native to america. Because of its unique biological characteristics and insect catching abnormal leaves, it has been favored by people. Bottle grass enters the Chinese market late, and the market demand is large, so it has important development value and broad market prospect. The traditional breeding methods such as ramets, cuttings and seeds are difficult to form large-scale production because of low propagation coefficient and long time consuming. Therefore, the rapid propagation of bottle grass by tissue culture is beneficial to the industrialization of bottle grass. At the same time, it is of important theoretical value to establish the regeneration system of bottle grass, and can provide practical technology for further research. In this study, Sarracenia single bud as the experimental material, effects of different concentrations of BA and TDZ on the proliferation of shoots compared with the bottle, optimized the concentration of NAA and discusses the basic culture of MS and N6 based on the concentration of bud proliferation effect. The results show that the concentration of BA in 1.6mg/L is suitable, the bud proliferation most, and no abnormal buds, but high concentrations of BA inhibited root growth; the concentration of TDZ in 0.1mg/L is the most appropriate, but the number of lateral buds proliferation than 1.6mg/L BA. The high concentration of TDZ was more severely inhibited root growth than BA, and produced a large bright yellow callus sorite base, bud shape is not normal; adding NAA on bud formation of root significantly promoted, but could not improve the proliferation rate; the proliferation effect of a large number of elements in the low concentration of MS element and N6 when the concentration ratio is good, relatively speaking, a large number of elements 20%MS than a large number of 20%N6 elements on the bud proliferation effect. The single bud as explants, effects of the addition of organic compounds on the growth of sarracenia. The results show that the enzymatic hydrolysis of casein, yeast extract, coconut water in the experimental concentration range have not been able to promote Sarracenia buds proliferation. Enzymatic hydrolysis of casein 500mg/L resulted in 16.67% dead buds, and the concentration of 2000mg/L, the mortality rate reached 53.85%; yeast extract 250mg/L led to 12.5% bud death, and when the concentration of 2000mg/L and the mortality rate is 64%; 5% of the coconut water led to 3% bud death, and at the concentration of 20% of the mortality rate reached 75%. The bottle body height is about 1.5cm in 60 healthy as materials, effects of basic medium, sucrose and auxin on Rooting of shoots. When the concentration of MS was 20%, the average root number, average root length and rooting rate were better than those of other concentrations, while N6 was the best with 40% concentration. Compared with a large amount of 20% MS elements, the average number of roots in the medium with large concentration of 40%N6 is larger, the average root length is larger, the rooting rate is high, and the roots are thicker and more hairy, which is beneficial to the subsequent transplanting life. The concentration of sucrose in the medium was best for 2.5%, and too low or high concentrations of sucrose were detrimental to the bottle, Cao Shenggen. Compared with IBA, NAA is more conducive to the bottle body in the grass root, add the appropriate concentration of NAA culture medium coarse root buds produced by medium, hair more conducive to transplant follow-up work. The addition of various concentrations of IBA on the basis of NAA did not contribute to rooting of the bottle grass. Callus was induced by adding 2, 4 - D0.8mg/L and TDZ2.7 mg/L on the leaves of pitcher grass. It was found that the callus formation rate of explants with higher maturity was the highest, reaching 60%. These calli were inoculated on the differentiation medium containing BA1mg/L and NAA0.1mg/L. After 60 days or so culture, the calli differentiated adventitious buds. These buds open then the seed multiplication medium, can continue to proliferate to form more buds, and these buds inserted into MS (20% light element concentration) +NAA0.1mg/L+ sucrose 2.5%+ agar 6g/L rooting medium, 20 days of bud appeared on the basal part of the red root process, 30 days, a complete root system was formed, and the rooting rate was 100%. [DOI]