[新版]骨髓间充质干细胞 嗅鞘细胞 联合移植 胶质瘢痕 神经纤维再生

[新版]骨髓间充质干细胞 嗅鞘细胞 联合移植 胶质瘢痕 神经纤维再生 骨髓间充质干细胞论文:BMSC与OEC联合移植对SCT大鼠神经元存活、胶质瘢痕以及神经再生的影响 【中文摘要】建立大鼠骨髓间充质干细胞(BMSCs)、嗅鞘细胞(OECs)的体外分离、纯化和培养,及大鼠全横断脊髓损伤模型,探讨BMSCs、OECs单独移植及BMSCs+OECs联合移植对全横断损伤大鼠脊髓?、?板层神经元存活的影响、及胶质瘢痕中GFAP和APC的表达和神经纤维再生的变化,为细胞移植治疗脊髓损伤提供理论和实验室依据。体外分离培养大鼠BMSCs和OECs并进行细胞染色鉴定;清洁级健康SD雄性大鼠25只,每组5只将动物随机分为假手术组(SG组)、手术对照组(CG组)、BMSC单独移植组、OEC单独移植组和BMSCs+OECs组。体外条件下将骨髓源性间充质干细胞(Dil标记)和OECs (Hoechst33342标记)立体定向移植入慢性期(全横断术后14天)全横断损伤大鼠脊髓的瘢痕处、头侧和尾侧。在脊髓损伤后第6m末灌注固定后取瘢痕处及头尾侧脊髓制作 石蜡切片,行苏木素染色及免疫组化染色, 分别计数各组脊髓?、?板层神经元数量及 面积;计数各组瘢痕中GFAP和APC阳性细 胞数量并分析其免疫染色OD值,同时观察宿 主PKCγ、5-HT及CGRP阳性纤维再生情况。 SPSS11.5软件包对数据进行统... 【英文摘要】[]:To investigate the effects of co-transplantation of bone marrow mesenchymal stem cells(BMSCs) and olfactory ensheathing cells(OECs) on the?and IX layers neural survival, glial scar and nerve regeneration in rats subjected to spinal cord transaction.[Methods]:Rat femurs derived BMSCs were isolated invitro and the passage3 cells were chosen to make immunocytochemistry stain for CD44; OECs were isolated and cultured from new born SD rat’s olfactory bulb and were identified by p75 immunostaining... 【关键词】骨髓间充质干细胞 嗅鞘细胞 联合移植 胶质瘢痕 神经纤维再生 【英文关键词】Bone marrow stromal cells olfactory ensheathing cells co-transplantation glial scar nerve fiber regeneration 【索购全文】联系Q1:138113721 Q2: 139938848