Diagnostic value and clinical significance of liver disease in patients with liver fibrosis four detection（肝脏疾病的诊断价值和临床意义四个检测肝纤维化患者）

Diagnostic value and clinical significance of liver disease in patients with liver fibrosis four detection（肝脏疾病的诊断价值和临床意义四个检测肝纤维化患者） Diagnostic value and clinical significance of liver disease in patients with liver fibrosis four detection [Abstract] Objective To investigate the serum fibrosis markers hyaluronic acid (HA type III procollagen (PC-III), type IV collagen (IV-C and laminin (LN) and the diagnostic value in chronic liver diseases and their clinical significance Methods 289 cases of inpatient and outpatient of our hospital from January 2007 to 2011 in April, 188 cases, 111 cases of normal control group of 42 cases for the healthy subjects, 30 males and 12 females. Results mild hepatitis B patients with HA, PC-III, IV IV-C, LN, and normal control group (t = 3.46, P <0.01); moderate hepatitis B, severe hepatitis B and liver cirrhosis in patients with serum HA, PC ? , IV C,, LN and normal control group comparison there are significant differences (t - = 5.18, P <0.001). Conclusion serum of HA, the PC-III, IV-C,, of LN content and liver damage degree of the relationship: cirrhosis of the liver> re degrees Hepatitis B> Hepatitis B moderate, mild hepatitis B detection of liver fibrosis in patients with chronic hepatitis B 4, 1 contribute to the observation and timely treatment, delaying the emergence of liver fibrosis and cirrhosis, and improve patients’ quality of life. [Key Words] liver fibrosis four chronic hepatitis, liver fibrosis, cirrhosis China hepatitis, up to 120 million carriers of hepatitis B virus, hepatitis B have 20% of hepatitis C of 60% in chronic hepatitis, cirrhosis and liver cancer, chronic hepatitis, cirrhosis of the liver substantive results lesions, generally considered more difficult to reverse the thus In addition to prescribe the right medicine in the treatment of lack of effective measures. Therefore, scholars invariably turned to liver fibrosis in hepatitis ? ? cirrhosis. the intermediate links of this “trilogy” - liver fibrosis of. 289 cases of chronic hepatitis, cirrhosis of the liver, serum hyaluronic acid (HA type III procollagen (PC-III and type IV collagen (IV-C and laminin protein (LN4 indicators for testing, to explore its relationship with chronic hepatitis the diagnostic value of liver fibrosis. 1 Materials and methods 1.1 General 2 information: select 289 cases of inpatient and outpatient, from January 2007 to 2011 in April, 188 cases of male and female 111 cases, aged 21-72 years old. Mild chronic hepatitis B, 213 cases of moderate to severe patients , 76 cases of patients with cirrhosis. diagnosis 2000 Xi’an, China Medical Association to amend the Viral Hepatitis Prevention Programme >> [1]. normal control group, 42 cases of hospital health examination, including 30 males and 12 females, age 25-69 years old. 1.2 Instruments and methods: measured by radioimmunoassay of serum HA, PC-III, IV-C, LN. Reagents provided by the Beijing North Biotechnology Institute University of Science and Technology of China Photoelectric Instruments GC-911 γ, measuring instruments - RIA counter operating in strict accordance with the instruments and reagents operating procedures. 1.3 Statistical analysis: All data x ? s, said the groups were analyzed using t test, P <0.01 for differences with SPSS 10.0 software processing. 3 2 Results Patients with mild hepatitis B HA, PC-III, IV-C, LN, and normal control group difference (t = 3.46, P <0.01); moderate hepatitis B, severe hepatitis B patients with cirrhosis of serum HA, PC III, IV C, LN, and normal control group (t = 5.18, P <0.001). test results and the progression of a positive correlation in Table 1. t values ​​compared with the normal control group: ? (t = 3.46, P <0.01) ? (t = 5.18, P <0.001) Share the free paper download center discussion , Due to the continuing role of immune dysfunction treatment factors that sustained liver cell damage in the process of development of chronic hepatitis, fibrous tissue hyperplasia. Into the liver fibrosis stage, the main pathological changes of collagen, protein polysaccharide and fibronectin and other extracellular matrix (ECM the intrahepatic excessive deposition [1], leading to liver fibrosis and even cirrhosis. HA belong to the protein polysaccharide uptake and degradation of acetic acid and lactic acid in the liver sinusoidal 4 endothelial cells, of HA increased production of hepatic fibrosis, and endothelial cell receptor decrease in degradation to reduce, plus collateral shunt into the systemic circulation, liver fibrosis, HA biggest changes was significantly higher, is a reflection of chronic liver fibrosis and inflammation changes in the most sensitive indicator of laminin (LN) is a quality non-jelly protein composition of the extracellular matrix, widely distributed in the transparent layer of the basement membrane and type IV collagen binding, the formation of the basement membrane skeleton, influence and regulate cell adhesion, movement, growth and differentiation of normal liver sinusoidal basement membrane, LN, content intrahepatic occurrence of chronic inflammatory injury, liver cells, endothelial cells and epithelial cells are involved in synthesis of LN, and liver sinus looked deposition [1.3]. type III procollagen (PC-III is a complete type III procollagen molecules, a direct reflection of the metabolic status of type III collagen, the PC-III is a PC-III NP generated by the enzyme under the action of the N-terminal peptide peptide, PC-III PC-III NP cracking into the blood, increased serum levels reflect the strong 5 synthesis of PC-III. continuous intrahepatic inflammation and necrosis of the PC-III increased serum levels pooled volume in and around the fibroblast number was significantly associated also closely related with the level of activity of liver fibrosis and inflammation of the liver necrosis is also related to, to reflect the dynamic process of liver fibrogenesis and the development of type IV collagen (IV-C for the basal collagen are mainly distributed in the liver, bile ducts, blood vessels lymphatic vessels around the space of Disse, normal liver sinus wall IV-C was not a continuous distribution, but the increase in IV-C synthesis and deposition in liver fibrosis, into the continuity of LN involved in sinusoidal capillarization formation and development of LN and IV-C co-exist in the basement membrane, the composition of the non-adhesive glycoproteins of liver fibrosis, LN aggregation and deposition in the hepatic sinusoids, promoting sinusoidal capillarization, the formation of portal hypertension. In this paper, the detection of 289 cases of chronic hepatitis, cirrhosis of the liver in patients with serum 6 hyaluronic acid (HA type III procollagen (PC-III, serum serum type I collagen (IV-C and laminin protein (LN4 item refers to reflect the serum HA, PC-III, IV-C, LN, content and degree of liver damage: liver cirrhosis with severe Hepatitis B> Hepatitis B moderate> mild hepatitis B detection of liver fibrosis in chronic hepatitis B patients 4 to help observation and prompt treatment to delay the emergence of liver fibrosis and cirrhosis, and improve the quality of life in patients with serum liver fibrosis four detection of liver fibrosis, a reliable indicator of cirrhosis of the liver, but also for judging liver fibrosis in clinical treatment provide a reliable basis. References [1] Liu Mingyou, Chen Yue, Yi Jianhua serum type III collagen measured in liver fibrosis [J]. Journal of Nuclear Medicine, 1995,15 (1) :132-134. [2] Wu Jun, Bao Lin Ping, He Boming. Determination of serum type IV collagen in liver fibrosis [J]. Shanghai Medical Laboratory Technology, 1998,13 (2:142-143. 7 Clinical of Xu Hongyue, Wang Xiao, Li love red serum laminin detection [J], [3] Application of Clinical Laboratory Science, 1996,14 (1:334. Links in the free paper download center 8