仙人掌论文：仙人掌 总黄酮 抗炎作用 血管活性胺 花生四烯酸代谢

仙人掌论文：仙人掌 总黄酮 抗炎作用 血管活性胺 花生四烯酸代谢 仙人掌论文:仙人掌 总黄酮 抗炎作用 血管活性胺 花生四烯酸代谢 【中文摘要】本文研究了仙人掌总黄酮的抗炎作用及抗炎机理。通过建立冰醋酸致小鼠腹腔毛细血管通透性增加,棉球致小鼠肉芽肿以及蛋清致大鼠足跖肿胀炎症模型,分别采用水提醇沉和超声波醇提两种方法提取的仙人掌总黄酮,用于考察不同方法提取的仙人掌总黄酮对不同炎症的作用效果。同时以水提醇沉法制得的仙人掌总黄酮作为试验,研究仙人掌抗炎作用的机理:采用荧光法测定蛋清致大鼠足跖肿胀炎性组织渗出液中组胺和5-HT的含量,研究仙人掌总黄酮对这两种血管活性胺释放的影响;通过人全血分析法测定仙人掌总黄酮对人抗凝全血中TXB2和PGI2含量的影响,从而反映其对COX-1和COX-2活性的影响;另外采用改良比色法考察仙人掌总黄酮对于5-LOX活性的影响。从花生四烯酸代谢的角度对仙人掌总黄酮的抗炎作用机理进行初步探讨。试验结果显示:1.水提醇沉高剂量组和超声波醇提高剂量组的仙人掌总黄酮均能极显著抑制冰醋酸所致的小鼠腹腔毛细血管通透性增加(P0.05);高、中、低剂量的仙人掌总黄酮均能使全血中PGI2的含量显著减少(P<0.05),其中低剂量的抑制作用最强(P<0.01),其作用效果与塞来昔布阳性药接近;此外,各剂量组的总黄酮对于5-LOX的酶活力也都具有极显著的抑制作用(P<0.01),且这种抑制作用在0.06-1.5mg?mL-1的浓度范围内呈现出一定的浓度依赖关系。说明仙人掌总黄酮能通过抑制COX和5-LOX的活性或表 达,达到阻断花生四烯酸代谢的效果,从而发挥抗炎的作用。 【英文摘要】This paper studies the anti-inflammatory effect of total flavonoids from Opuntia monacantha (Willd) Haw and its mechanisms of action. To investigate the anti-inflammatory effect of total flavonoids from Opuntia monacantha (Willd.) Haw extracted by two different processes (water extraction-alcohol precipitation method/WEAP and ultrasonic wave-alcohol extraction method/UWAE) through the models of acetic acid-induced peritoneal capillary permeability in mice, cotton-pellet granuloma of the mice and egg white-induced rat paw oedema. Moreover, total flavonoids extracted by WEAP method were used in the study of anti-inflammatory mechanism of Opuntia monacantha (Willd) Haw: The effect of total flavonoids from Opuntia monacantha (Willd) Haw on the release of vasoactive amines was investigated by fluorescence method to detect the histamine and 5-Hydroxytryptamine (5-HT) contents in inflammatory exudate of paw edema induced by egg white in rats.To investigate the effect of total flavonoids from Opuntia monacantha (Wild.) Haw on COX-1 and COX-2 activity, the contents of TXB2 and PGI2 in erparinized whole blood were detected based on human whole blood assays. And the modified colorimetric method was used to observe the effect of total flavonoids on 5-LOX activity, in order to discuss the anti-inflammatory mechanism of total flavonoids from the perspective of arachidonic acid metabolism.Results:1. High-dosage of both WEAP and UWAE total flavonoids had significant inhibitory action on penetration of capillary vessel in mice(P0.05). Total flavonoids from Opuntia monacantha (Willd.) Haw made a significant decrease of PGI2 content in erparinized whole blood (P<0.05) in all doses, especially the low dose of total flavonoids (P<0.01) of which the effect was approximate to that of celecoxib. In addition, each dose of total flavonoids showed a significant inhibition of 5-LOX activity (P<0.01), and this effect on inhibition showed a certain concentration dependent manner within the concentration range from 0.06 to 1.5 mg?mL-1. The results indicated that total flavonoids from Opuntia monacantha (Willd.) Haw could inhibit COX and 5-LOX activity or expression to block arachidonic acid metabolism which played a important role in inflammation. 【关键词】仙人掌 总黄酮 抗炎作用 血管活性胺 花生四烯酸代 谢 【采买全文】 1.3.9.9.38.8.4.8 1.3.8.1.13.7.2.1 同时提供论文写作一对一辅导和论文发表服务.保过包发. 【说明】本文仅为中国学术文献总库合作提供，无涉版权。作者如有异议请与总库或学校联系。 【英文关键词】Opuntia monacantha (Willd.) Haw Total flavonoids Anti- inflammatory effect Vasoactive amines Arachidonic acid metabolism 【目录】仙人掌总黄酮抗炎作用及机理的研究 摘要 4-6 Abstract 6-7 常用符号及缩略语说明 8-11 第一部分 选题背景与文献综述 11-26 第一章 选题背景 11-14 1. 仙人掌的抗炎效果 11 2. 仙人掌抗炎活性部 位的研究 11-12 3. 仙人掌抗炎机理的研究 12-13 4.试 验目的与意义 13-14 第二章 文献综述 14-26 1. 仙人 掌概述 14 2. 仙人掌的化学成分 14-17 3. 黄酮类化合 物抗炎机理的研究进展 17-26 第二部分 试验研究 26-46 第一章 仙人掌总黄酮抗炎作用的研究 26-33 1. 试验材料 26-27 2. 试验方法 27-28 3. 试验结果 28-31 4. 讨论 31-33 第二章 仙人掌总黄酮对蛋清致 大白鼠足趾肿胀炎性组织中组胺和5-HT含量的影响 33-39 1. 试验材料 33-34 2. 试验方法 34-35 3. 试验结果 35-37 4. 讨论 37-39 第三章 仙人掌总黄酮对花生四 烯酸代谢途径的影响 39-46 1. 试验材料 39-40 2. 试 验方法 40-42 3. 试验结果 42-44 4. 讨论 44-46 第三部分 结论与创新 46-48 1. 结论 46 2. 创新 46-48 参考文献 48-59 致谢 59