人磷酸化细胞外信号调节激酶,pERK,ELISA试剂盒定量分析

人磷酸化细胞外信号调节激酶,pERK,ELISA试剂盒定量 Guinea pig, pig, dog, cow, sheep, monkey, rabbit, etc. Sample complete: cell culture supernatant, serum, plasma, urine, tissue fluid, atrial water, body fluid specimens, etc. Cytokine series detection kit tumor marker series detection kit autoimmune series detection kit Heart disease series detection kit endocrine series detection kit myocardial infarction series detection kit Liver fibrosis series detection kit infectious disease series detection kit microbial infectious disease series detection kit A series of cellular immune detection kit eugenics detection special protein detection人磷酸化细胞外信号 调节激酶,pERK,ELISA试剂盒定量分析 ELISA实验原理是:1)使抗原或抗体结合到某种固相载体面，并保持其免疫活性。2)使抗原或抗体与某种酶连接成酶标抗原或抗体， 这种酶标抗原或抗体既保留其免疫活性，又保留酶的活性。在测定时，把人磷酸化细胞外信号调节激酶(pERK)ELISA检测试剂盒受检 标本(测定其中的抗体或抗原)和酶标抗原或抗体按不同的步骤与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成 的抗原抗体复合物与其他物质分开，最后结合在固相载体上的酶量与标本中受检物质的量成一定的比例。加入酶反应的底物后，底物被 酶催化变为有色产物，产物的量与标本中受检物质的量直接相关，故可根据颜色反应的深浅进行定性或定量分析。由于酶的催化效率很 高，故可极大地放大反应效果，从而使测定方法达到很高的敏感度。人磷酸化细胞外信号调节激酶,pERK,ELISA试剂 盒定量分析Technical principle of ELISA detection kit: (1) solid phase of antigen or antibody and enzyme labeling of antigen or antibody. (2) antigen or antibody that remains on the surface of the solid carrier remains immunological activity. (3) the enzyme labeled antigen or antibody retains its immunological activity and retains the activity of the enzyme. (4) the tested specimen reacts with the antigen or antibody on the solid support surface. The enzyme labeled antigen or antibody is also bound to the solid carrier by reaction. (5) the amount of enzyme present on solid phase is proportional to the amount of substance detected in the sample. (6) after adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the quantity of the detected substance in the sample. The method has high sensitivity (pg-ng/ml level) and good repeatability. A highly sensitive experimental technique based on immunological reaction, which combines the specific reaction of antigen and antibody with the efficient catalysis of enzyme to substrate.