膝关节后交叉韧带断裂对内侧副韧带组织学的影响

膝关节后交叉韧带断裂对内侧副韧带组织学的影响 中国研究组组组组组组 2013, Vol. 17 Issue (2): 280-285 DOI: 10.3969/j.issn.2095-4344.2013.02.017 组组构建与生物力学 膝关关后交叉关关裂关关副关关关关的影断内学响 Effect of posterior cruciate ligament rupture on histological features of medial collateral ligament 全文: PDF 摘要 背景:通比?型胶原、?组组组型胶原相数量和?组组组组/?型胶原的比可以一定程度上组组组组组组组组 判断的学性能。组组组组组组组组组 目的:察膝后交叉断裂兔保持低生理荷和活度组组组组组组组组组组组组组组组组组组组组组组组组组组 内副学的化。组组组组组组组组组组组 :24只成年雄性家兔双膝配自身照,行后交叉完组组组组组组组组组组组组组组组组组组组组组组 全切断,照只暴露后交叉而不切断,造模后第组组组组组组组组组组组组组组组组组组组组8,16,24,40周随机死组组6只组组 兔。行木精组组组组组-伊染色,天狼猩染色?型和?组组组组组组组组组组组组型胶原的相数量。组组组组 组组组组果与:?组木精-伊染色果:组组组组组组8,16,24周两内副胶原分布、组组组组组组组组组组组 排列无明差,组组组组40周胶原照稀疏。?组组组组组组组组组组组组组组组天狼猩染色果:组组组组组组8,16,24 周内副的?型和?组组组组组组组组组型胶原和分照著增加组组组组组组组组组组组组组组(P < 0.05),40周组 组组组组组组组组组组照著减少(P < 0.05),8周和照之内副的?组组组组组组组组组组组组组组组/?型胶原组组 的比差异无著性意组组组组组组组组组(P > 0.05),16,24,40周比著分小于照组组组组组组组组组组组组组组组(P < 0.05)。明兔膝后交叉后短期内内副学特性组组组组组组组组组组组组组组组组组组组组组组组组组组组组 无明影响,随着延,学特性著下降。组组组组组组组组组组组组组组组组组组组组组 组组组 : 组组构建, 组组构建与生物力学, 膝组组, 后交叉组组, 断裂, 内副组组组组, 胶原组组, 组组学特征, 组组组修, 组组组组组组构建片文章, Abstract: BACKGROUND: By comparing the relative quantity of type? and III collagens and type ?/? collagen ratio, we can determine the ligament histology performance to some extent. OBJECTIVE: To explore the histological effect of posterior cruciate ligament injury on the medial collateral ligament under a lower physiological load in rabbits. METHODS: Bilateral knees of 24 male rabbits were enrolled for a self-control trial. Posterior cruciate ligaments at the experimental side were transected, and those in the control side were only exposed but not transected. At the 8th, 16th, 24th and 40th weeks after modeling, six rabbits were executed randomly, and then the histological alteration of the medial collateral ligament was observed through hematoxylin-eosin staining and picrosirius-polarization staining. RESULTS AND CONCLUSION: (1)Hematoxylin-eosin staining: At the 8th, 16thand 24th weeks, there was no obvious difference in the collagen distribution and arrangement of the medial collateral ligament between experimental group and control group; at the 40th week, the collagen was thinner in the experimental group. (2)Picrosirius-polarization staining: At the 8th, 16th and 24th weeks, the total number of type ? and ? collagens in the medial collateral ligament of the experiment group was more than that in the control group (P < 0.05); however, at the 40th week, the total number of type I and III collagens in the medial collateral ligament of the experimental group was much fewer than that of the control group (P < 0.05); at the 8th week, ratio of type ?/? collagen in the medial collateral ligament of the experimental group did not differ from that in the control group (P > 0.05); however, at the 16th, 24th and 40th weeks, the ratio of type ?/? collagen in the experimental group was much fewer than that in the control group (P < 0.05). In short-term, posterior cruciate ligament injury has no significant effect on histological properties of the rabbit medial collateral ligament. However, the histological properties can decrease significantly with time. Key words: tissue construction; tissue construction and biomechanics; knee; posterior cruciate ligament; fracture; medial collateral ligament;collagen fibers; histological features; ligament repair; tissue construction photographs- containing paper; 收稿日期: 2012-05-16 研究亮点: 1 建立物模型,后交叉切断后膝未予固定,组组组组组组组组组组组组组组组组组组组组组组组组组 兔能自由活,但由于空小,膝活范有限,荷组组组组组组组组组组组组组组组组组组组组组组组组组组组 一般低生理荷。组组组组组组组组 2 通学察在短期内后交叉断裂的内副胶原组组组组组组组组组组组组组组组组组组组组组组组组组组组组 组组组组组组组组组组组的数量完整增加,但是?型/?型胶原的比与后交叉完整组组组组组组组组组组组组组 组组组物存在差。