PBI121全序列,NCBI的登陆号：AF485783

PBI121全序列,NCBI的登陆号：AF485783 全序列～的登陆,号pBI121NCBIAF485783 My NCBI [Sign In] [Register] Goþÿ Search for You need JavaScript to work with this page. LimitsPreview/IndexHistoryClipboardDetails þÿþÿþÿ Display Show beginRefreshFeatures: SNP CDD Range: from to endMGC HPRD STS tRNA Reverse complemented strand Links1: AF485783. Reports Binary vector pBI...[gi:19569229] LOCUS AF485783 14758 bp DNA circular SYN 15-MAY- 2003 DEFINITION Binary vector pBI121, complete sequence.ACCESSION AF485783 VERSION AF485783.1 GI:19569229 KEYWORDS . SOURCE Binary vector pBI121 ORGANISM Binary vector pBI121 other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 14758) AUTHORS Chen,P.Y., Wang,C.K., Soong,S.C. and To,K.Y. TITLE Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants JOURNAL Mol. Breed. 11, 287-293 (2003) REFERENCE 2 (bases 1 to 14758) AUTHORS To,K.Y. TITLE Direct Submission JOURNAL Submitted (20-FEB-2002) Institute of BioAgricultural Sciences, Academia Sinica, Taipei 11529, TaiwanFEATURES Location/Qualifiers source 1..14758 /organism="Binary vector pBI121" /mol_type="genomic DNA" /db_xref="taxon:189807" /note="constructed using pB221 from Clontech Laboratories and Bin19 described in GenBank Accession Number U09365" misc_feature complement(13..796) /note="similar to traF in GenBank Accession Number X54459" rep_origin complement(790..1168) /note="ColE1 ori; similar to sequence in GenBank Accession Number V00268" misc_feature complement(1161..2344) /note="similar to tetA in GenBank Accession Number X75761" misc_feature complement(2454..2478) /note="T-DNA right border" promoter 2519..2825 /note="NOS" gene 2838..3632 /gene="nptII" CDS 2838..3632 /gene="nptII" /codon_start=1 /product="neomycin phosphotransferase II" /protein_id="AAL92039.1" /db_xref="GI:19569230" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" terminator 4022..4277 /note="NOS" promoter 4974..5808 /note="CaMV 35S" gene 5845..7656 /gene="gusA" CDS 5845..7656 /gene="gusA" /note="GUS" /codon_start=1 /product="beta-glucuronidase" /protein_id="AAL92040.1" /db_xref="GI:19569231" /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ" terminator 7727..7979 /note="NOS" misc_feature complement(8621..8646) /note="T-DNA left border" misc_feature complement(9156..10198) /note="similar to tetA in GenBank Accession Number L13842" misc_feature complement(10199..11680) /note="similar to trfA in GenBank Accession Number X00713" misc_feature complement(11681..12673) /note="similar to NPTIII gene in GenBank Accession Number V01547" misc_feature complement(12674..13443) /note="similar to transposable element IS1 in GenBank Accession Number X58999" misc_feature complement(13444..13794) /note="similarity to NPT III gene in GenBank Accession Number V01547" misc_feature complement(13795..14066) /note="similar to kilA in GenBank Accession Number M62846" rep_origin complement(14141..14758) /note="ori V; similar to sequence in GenBank Accession Number M20134" ORIGIN 1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc 61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc 121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat 181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc 241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag 301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc 361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc 421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg 481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc 541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata 601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg 661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata 721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta 781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg 1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga 1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg 1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac 1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc 1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg 1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc 1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc 1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg 1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg 1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg 1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg 1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc 1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa 1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg 2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa 2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc 2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc 2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg 2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc 2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct 2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc 2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc 2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc 2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa 2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg 2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga 3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat 3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg 3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg 3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc 4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc 4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg 4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata 4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc 4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg 4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag 4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta 4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca 4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt 4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg 4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt 4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc 4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag 4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg 5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa 5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat 5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc 5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat 5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt 5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca 5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat 5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat 5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg 5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc 5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt 5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca 5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga 5821 ggatccccgg gtggtcagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc 5881 aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag 5941 cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac 6001 gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa 6061 gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact 6121 cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg 6181 ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt 6241 tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa 6301 aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc 6361 agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat 6421 gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc 6481 agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg 6541 actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg 6601 tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg 6661 tcagtggcag tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc 6721 tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg 6781 cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct 6841 tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact 6901 gctgctgtcg gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa 6961 gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt 7021 aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac 7081 gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg 7141 cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac 7201 accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat 7261 gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg 7321 caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg 7381 ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg 7441 tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc 7501 gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact 7561 cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac 7621 ttcggtgaaa aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcacca 7681 tcgtcggcta cagcctcggg aattgctacc gagctcgaat ttccccgatc gttcaaacat 7741 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata 7801 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat 7861 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa 7921 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg 7981 ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 8041 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 8101 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc 8161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 8221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat 8281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 8341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc 8401 tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa caggattttc 8461 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 8521 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa 8581 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc 8641 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat 8701 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact 8761 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac 8821 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg 8881 atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct 8941 taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata 9001 aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac 9061 tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc 9121 ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac 9181 aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc 9241 gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg 9301 atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt 9361 aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat 9421 ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt 9481 caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg 9541 tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc 9601 taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa 9661 tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat 9721 ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc 9781 gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc 9841 gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta 9901 ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc 9961 ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat 10021 ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat 10081 tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga 10141 tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg 10201 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc 10261 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct 10321 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg 10381 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt 10441 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca 10501 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca 10561 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg 10621 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa 10681 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc 10741 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca 10801 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa 10861 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca 10921 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac 10981 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg 11041 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata 11101 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga 11161 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg 11221 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg 11281 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc 11341 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct 11401 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct 11461 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc 11521 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc 11581 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag 11641 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc 11701 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa 11761 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc 11821 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt 11881 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc 11941 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc 12001 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc 12061 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa 12121 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata 12181 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg 12241 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc 12301 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata 12361 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc 12421 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc 12481 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat 12541 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca 12601 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg 12661 aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga 12721 ggtgctccag tggcttctgt gtctatcagc tgtccctcct gttcagctac tgacggggtg 12781 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa 12841 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt 12901 gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc 12961 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc 13021 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta 13081 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc 13141 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct 13201 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa 13261 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga 13321 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc 13381 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta 13441 cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc 13501 catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc 13561 cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata tattatctta 13621 tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct 13681 tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt 13741 tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc 13801 cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg 13861 gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca 13921 ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca 13981 cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc 14041 cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg 14101 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg 14161 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca 14221 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 14281 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 14341 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat 14401 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 14461 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 14521 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 14581 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 14641 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct 14701 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggRestriction analysis on DNAMAN1 Methylation: dam-No dcm-No Screened with 117 enzymes, 384 sites found AatII 2 GACGT/C 2216 13033 AccIII 4 T/CCGGA 1439 2081 5486 13120 AclI 3 AA/CGTT 1367 2610 9044 AflII 2 C/TTAAG 4051 7756 AhaIII 8 TTT/AAA 2414 2492 11959 11988 12355 12515 13692 14240 Alw44I 2 G/TGCAC 6778 7103 AlwNI 9 CAGNNN/CTG 1725 6687 9588 10535 10709 12769 13328 13972 14110 ApaBI 4 GCANNNNN/TGC 1726 1792 8761 14056 ApaI 1 GGGCC/C 3903 ApaLI 2 G/TGCAC 6778 7103 AsuII 2 TT/CGAA 3648 6938 BalI 4 TGG/CCA 3048 6466 12907 14519 BamHI 1 G/GATCC 5821 BbeI 4 GGCGC/C 2342 2969 8140 9905 BbvII 6 GAAGACNN/ 5451 5676 9872 13131 5679 11991 BclI 4 T/GATCA 1336 2518 5934 8879 BglI 10 GCCNNNN/NGGC 224 298 301 1182 1814 8134 9625 11038 13890 13919 BglII 2 A/GATCT 2344 9148 Bpu1102I 2 GC/TNAGC 2655 12063 Bsc91I 6 GAAGACNN/ 5451 5676 9872 13131 5679 11991 BsiI 7 C/TCGTG 3558 9961 863 3684 5647 9736 13805 BsmI 10 GAATGCN/ 1796 5005 9913 13843 13886 1742 2231 5902 10659 12571 Bsp1407I 2 T/GTACA 6965 7391 BspHI 4 T/CATGA 177 2521 6729 12213 BspMI 13 ACCTGCNNNN/ 3234 3684 7177 10336 10786 11145 13980 381 2853 4955 5027 6447 14236 BspMII 4 T/CCGGA 1439 2081 5486 13120 BssHII 10 G/CGCGC 1696 1698 2285 3363 4222 4246 7927 7951 10119 13546 BstD102I 14 GAG/CGG 1491 2527 3633 4903 9560 10397 10923 13301 625 1107 3579 8143 9236 10046 BstEII 1 G/GTNACC 13001 BstXI 1 CCANNNNN/NTGG 12671 Bsu36I 1 CC/TNAGG 2700 ClaI 1 AT/CGAT 4537 Csp45I 2 TT/CGAA 3648 6938 CspI 2 CG/GWCCG 1442 3482 CvnI 1 CC/TNAGG 2700 DraI 8 TTT/AAA 2414 2492 11959 11988 12355 12515 13692 14240 DraIII 1 CACNNN/GTG 8290 DrdI 5 GACNNNN/NNGTC 934 2993 5408 8334 13035 EagI 15 C/GGCCG 121 414 1193 1608 1650 2872 3855 9339 10041 11177 13891 14092 14535 14662 14723 Eam1105I 1 GACNNN/NNGTC 8953 Ecl136II 1 GAG/CTC 7713 Eco31I 1 GGTCTCN/ 5045 Eco47III 5 AGC/GCT 9466 10199 12814 13092 14063 Eco52I 15 C/GGCCG 121 414 1193 1608 1650 2872 3855 9339 10041 11177 13891 14092 14535 14662 14723 Eco56I 17 G/CCGGC 412 538 548 1522 1537 1684 1871 2335 3466 3749 8182 9752 11070 11661 13857 14533 14657 Eco57I 8 CTGAAGNNNNNNNNNNNNNNNN/ 1751 1874 2517 3112 3544 6866 13272 9985 EcoICRI 1 GAG/CTC 7713 EcoNI 1 CCTNN/NNNAGG 1627 EcoRI 1 G/AATTC 7982 EcoRV 8 GAT/ATC 3838 3964 5713 6402 6633 9054 11790 13591 EheI 4 GGC/GCC 2340 2967 8138 9903 EspI 2 GC/TNAGC 2655 12063 HindIII 1 A/AGCTT 4950 MfeI 3 C/AATTG 5457 5975 10077 Mlu113I 2 CC/GCGG 2631 14666 MluI 4 A/CGCGT 6440 7138 7156 13105 MscI 4 TGG/CCA 3048 6466 12907 14519 MstI 5 TGC/GCA 2271 3068 8124 9695 13138 MstII 1 CC/TNAGG 2700 NaeI 17 GCC/GGC 414 540 550 1524 1539 1686 1873 2337 3468 3751 8184 9754 11072 11663 13859 14535 14659 NarI 4 GG/CGCC 2339 2966 8137 9902 NcoI 4 C/CATGG 3398 5277 9417 9945 NdeI 1 CA/TATG 10475 NheI 1 G/CTAGC 2715 NotI 1 GC/GGCCGC 14662 NruI 3 TCG/CGA 5918 7562 9198 NsiI 3 ATGCA/T 4142 7847 9128 PflMI 1 CCANNNN/NTGG 10709 PmeI 1 GTTT/AAAC 2492 PstI 3 CTGCA/G 3019 4966 12854 PvuI 3 CGAT/CG 4024 7729 8105 PvuII 5 CAG/CTG 3072 4771 8074 8531 12749 RleAI 5 CCCACANNNNNNNNNNNN/ 5025 10248 5887 6124 14460 SacI 1 GAGCT/C 7715 SacII 2 CCGC/GG 2633 14668 SapI 5 GCTCTTCN/ 76 447 1153 3310 3520 SauI 1 CC/TNAGG 2700 ScaI 1 AGT/ACT 5179 SfiI 1 GGCCNNNN/NGGCC 11038 SgrAI 4 CR/CCGGYG 538 10821 11070 14657 SmaI 1 CCC/GGG 5828 SnaBI 1 TAC/GTA 6229 SphI 3 GCATG/C 2371 3371 4960 SpoI 3 TCG/CGA 5918 7562 9198 SspI 6 AAT/ATT 2724 4671 7114 9669 11717 14232 SstI 1 GAGCT/C 7715 SstII 2 CCGC/GG 2633 14668 Tth111I 5 GACN/NNGTC 1600 2146 3084 8725 13386 VspI 3 AT/TAAT 4763 4822 6793 XbaI 1 T/CTAGA 5815 XcmI 4 CCANNNNN/NNNNTGG 9710 10197 12319 14467 XmaI 1 C/CCGGG 5826 XmaIII 15 C/GGCCG 121 414 1193 1608 1650 2872 3855 9339 10041 11177 13891 14092 14535 14662 14723 XmnI 3 GAANN/NNTTC 4659 5378 5673 XorII 3 CGAT/CG 4024 7729 8105 List by Site Order 76 SapI 2853 BspMI 6729 BspHI 11038 BglI 121 Eco52I 2872 XmaIII 6778 Alw44I 11070 SgrAI 121 XmaIII 2872 EagI 6778 ApaLI 11070 Eco56I 121 EagI 2872 Eco52I 6793 VspI 11072 NaeI 177 BspHI 2966 NarI 6866 Eco57I 11145 BspMI 224 BglI 2967 EheI 6938 AsuII 11177 Eco52I 298 BglI 2969 BbeI 6938 Csp45I 11177 XmaIII 301 BglI 2993 DrdI 6965 Bsp1407I 11177 EagI 381 BspMI 3019 PstI 7103 ApaLI 11661 Eco56I 412 Eco56I 3048 BalI 7103 Alw44I 11663 NaeI 414 NaeI 3048 MscI 7114 SspI 11717 SspI 414 XmaIII 3068 MstI 7138 MluI 11790 EcoRV 414 Eco52I 3072 PvuII 7156 MluI 11959 DraI 414 EagI 3084 Tth111I 7177 BspMI 11959 AhaIII 447 SapI 3112 Eco57I 7391 Bsp1407I 11988 DraI 538 Eco56I 3234 BspMI 7562 NruI 11988 AhaIII 538 SgrAI 3310 SapI 7562 SpoI 11991 BbvII 540 NaeI 3363 BssHII 7713 EcoICRI 11991 Bsc91I 548 Eco56I 3371 SphI 7713 Ecl136II 12063 Bpu1102I 550 NaeI 3398 NcoI 7715 SstI 12063 EspI 625 BstD102I 3466 Eco56I 7715 SacI 12213 BspHI 863 BsiI 3468 NaeI 7729 PvuI 12319 XcmI 934 DrdI 3482 CspI 7729 XorII 12355 AhaIII 1107 BstD102I 3520 SapI 7756 AflII 12355 DraI 1153 SapI 3544 Eco57I 7847 NsiI 12515 AhaIII 1182 BglI 3558 BsiI 7927 BssHII 12515 DraI 1193 Eco52I 3579 BstD102I 7951 BssHII 12571 BsmI 1193 XmaIII 3633 BstD102I 7982 EcoRI 12671 BstXI 1193 EagI 3648 AsuII 8074 PvuII 12749 PvuII 1336 BclI 3648 Csp45I 8105 PvuI 12769 AlwNI 1367 AclI 3684 BsiI 8105 XorII 12814 Eco47III 1439 BspMII 3684 BspMI 8124 MstI 12854 PstI 1439 AccIII 3749 Eco56I 8134 BglI 12907 MscI 1442 CspI 3751 NaeI 8137 NarI 12907 BalI 1491 BstD102I 3838 EcoRV 8138 EheI 13001 BstEII 1522 Eco56I 3855 Eco52I 8140 BbeI 13033 AatII 1524 NaeI 3855 XmaIII 8143 BstD102I 13035 DrdI 1537 Eco56I 3855 EagI 8182 Eco56I 13092 Eco47III 1539 NaeI 3903 ApaI 8184 NaeI 13105 MluI 1600 Tth111I 3964 EcoRV 8290 DraIII 13120 AccIII 1608 EagI 4024 XorII 8334 DrdI 13120 BspMII 1608 XmaIII 4024 PvuI 8531 PvuII 13131 BbvII 1608 Eco52I 4051 AflII 8725 Tth111I 13131 Bsc91I 1627 EcoNI 4142 NsiI 8761 ApaBI 13138 MstI 1650 XmaIII 4222 BssHII 8879 BclI 13272 Eco57I 1650 EagI 4246 BssHII 8953 Eam1105I 13301 BstD102I 1650 Eco52I 4537 ClaI 9044 AclI 13328 AlwNI 1684 Eco56I 4659 XmnI 9054 EcoRV 13386 Tth111I 1686 NaeI 4671 SspI 9128 NsiI 13546 BssHII 1696 BssHII 4763 VspI 9148 BglII 13591 EcoRV 1698 BssHII 4771 PvuII 9198 SpoI 13692 DraI 1725 AlwNI 4822 VspI 9198 NruI 13692 AhaIII 1726 ApaBI 4903 BstD102I 9236 BstD102I 13805 BsiI 1742 BsmI 4950 HindIII 9339 Eco52I 13843 BsmI 1751 Eco57I 4955 BspMI 9339 XmaIII 13857 Eco56I 1792 ApaBI 4960 SphI 9339 EagI 13859 NaeI 1796 BsmI 4966 PstI 9417 NcoI 13886 BsmI 1814 BglI 5005 BsmI 9466 Eco47III 13890 BglI 1871 Eco56I 5025 RleAI 9560 BstD102I 13891 EagI 1873 NaeI 5027 BspMI 9588 AlwNI 13891 XmaIII 1874 Eco57I 5045 Eco31I 9625 BglI 13891 Eco52I 2081 BspMII 5179 ScaI 9669 SspI 13919 BglI 2081 AccIII 5277 NcoI 9695 MstI 13972 AlwNI 2146 Tth111I 5378 XmnI 9710 XcmI 13980 BspMI 2216 AatII 5408 DrdI 9736 BsiI 14056 ApaBI 2231 BsmI 5451 BbvII 9752 Eco56I 14063 Eco47III 2271 MstI 5451 Bsc91I 9754 NaeI 14092 EagI 2285 BssHII 5457 MfeI 9872 BbvII 14092 XmaIII 2335 Eco56I 5486 AccIII 9872 Bsc91I 14092 Eco52I 2337 NaeI 5486 BspMII 9902 NarI 14110 AlwNI 2339 NarI 5647 BsiI 9903 EheI 14232 SspI 2340 EheI 5673 XmnI 9905 BbeI 14236 BspMI 2342 BbeI 5676 Bsc91I 9913 BsmI 14240 AhaIII 2344 BglII 5676 BbvII 9945 NcoI 14240 DraI 2371 SphI 5679 Bsc91I 9961 BsiI 14460 RleAI 2414 DraI 5679 BbvII 9985 Eco57I 14467 XcmI 2414 AhaIII 5713 EcoRV 10041 EagI 14519 BalI 2492 DraI 5815 XbaI 10041 XmaIII 14519 MscI 2492 AhaIII 5821 BamHI 10041 Eco52I 14533 Eco56I 2492 PmeI 5826 XmaI 10046 BstD102I 14535 NaeI 2517 Eco57I 5828 SmaI 10077 MfeI 14535 EagI 2518 BclI 5887 RleAI 10119 BssHII 14535 XmaIII 2521 BspHI 5902 BsmI 10197 XcmI 14535 Eco52I 2527 BstD102I 5918 SpoI 10199 Eco47III 14657 Eco56I 2610 AclI 5918 NruI 10248 RleAI 14657 SgrAI 2631 Mlu113I 5934 BclI 10336 BspMI 14659 NaeI 2633 SacII 5975 MfeI 10397 BstD102I 14662 NotI 2633 SstII 6124 RleAI 10475 NdeI 14662 XmaIII 2655 Bpu1102I 6229 SnaBI 10535 AlwNI 14662 Eco52I 2655 EspI 6402 EcoRV 10659 BsmI 14662 EagI 2700 Bsu36I 6440 MluI 10709 AlwNI 14666 Mlu113I 2700 MstII 6447 BspMI 10709 PflMI 14668 SacII 2700 SauI 6466 BalI 10786 BspMI 14668 SstII 2700 CvnI 6466 MscI 10821 SgrAI 14723 EagI 2715 NheI 6633 EcoRV 10923 BstD102I 14723 Eco52I 2724 SspI 6687 AlwNI 11038 SfiI 14723 XmaIII Non Cut Enzymes Acc65I AgeI AscI Asp718I AvrII Eco72IFseI HpaI I-PpoI KpnI PacI PinAIPmaCI SalI SciI SpeI SplI SrfIStuI SunI SwaI XhoI Restriction sites on DNAMAN1 1 TGAGCGTCGCAAAGGCGCTCGGTCTTGCCTTGCTCGTCGGTGATGTACTTCACCAGCTCC ACTCGCAGCGTTTCCGCGAGCCAGAACGGAACGAGCAGCCACTACATGAAGTGGTCGAGG SapI 61 GCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGCGCACCCCC CGCTTCAGCGAGAAGAACTACCTCGCGTACCCCTGCACGAACCGTTAGTGCGCGTGGGGG EagI XmaIII Eco52I BspHI 121 CGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCAT GCCGGCAAAATCGCCGATTTTTTCAGTACCGAGACGGGAGCCCGCCTGGTGCGGGTAGTA BglI 181 GACCTTGCCAAGCTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGC CTGGAACGGTTCGAGCAGGACGAAGAGAAGCTAGAAGCGGTCGTCCCGCTCCTAGCACCG BglI 241 ATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAG TAGTGGCTTGGCGCGGCACGCGCCCAGCAGCCACTCGGTCTCAAAGTCGTCCGGCGGGTC BglI 301 GCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCC CGCCGGGTCCAGCGGTAACTACGCCCGGTCGAGCGCCTGCACGAGTATCAGGTGCTGCGG EagI Eco52I XmaIII NaeI BspMI Eco56I 361 CGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGC GCACTAAAACATCGGGACCGGCTGCCGGTCGTCCATCCGGCTGTCCGAGTACGGCCGGCG SapI 421 CGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGG GCGGCGGAAAAGGAGTTAGCGAGAAGCAAGCAGACCTTCCGTCATGTGGAACTATCCACC NaeI SgrAI Eco56I 481 GCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCC CGACGGGAAGGACCAACCGAACCAAAGTAGTCGGTAGGCGAACGGGAGTAGACAATGCGG NaeI Eco56I 541 GGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATA CCGCCATCGGCCGGTCGGAGCGTCTCGTCCTAAGGGCAACTCGTGGCGGTCCACGCTTAT BstD102I 601 AGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG TCCCTGTCACTTCTTCCTTGTGGGCGAGCGCCCACCCGGATGAAGTGGATAGGACGGGCC 661 CTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATA GACTGCGGCAACCTATGTGGTTCCTTTCAGATGTGCTTGGGAAACCGTTTTAGGACATAT 721 TCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTA AGCACGCTTTTTCCTACCTATATGGCTTTTTTAGCGATATTACTGGGGCTTCGTCCCAAT 781 TGCAGCGGAAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG ACGTCGCCTTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGC BsiI 841 GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTT CGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAA DrdI 901 ATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAG TATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTC 961 GGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTT CCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAA 1021 GCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTA CGACCGGAAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCAT BstD102I 1081 TTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGT AATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCA EagI XmaIII SapI BglI Eco52I 1141 CAGTGAGCGAGGAAGCGGAAGAGCGCCAGAAGGCCGCCAGAGAGGCCGAGCGCGGCCGTG GTCACTCGCTCCTTCGCCTTCTCGCGGTCTTCCGGCGGTCTCTCCGGCTCGCGCCGGCAC 1201 AGGCTTGGACGCTAGGGCAGGGCATGAAAAAGCCCGTAGCGGGCTGCTACGGGCGTCTGA TCCGAACCTGCGATCCCGTCCCGTACTTTTTCGGGCATCGCCCGACGATGCCCGCAGACT 1261 CGCGGTGGAAAGGGGGAGGGGATGTTGTCTACATGGCTCTGCTGTAGTGAGTGGGTTGCG GCGCCACCTTTCCCCCTCCCCTACAACAGATGTACCGAGACGACATCACTCACCCAACGC BclI AclI 1321 CTCCGGCAGCGGTCCTGATCAATCGTCACCCTTTCTCGGTCCTTCAACGTTCCTGACAAC GAGGCCGTCGCCAGGACTAGTTAGCAGTGGGAAAGAGCCAGGAAGTTGCAAGGACTGTTG AccIII BspMII 1381 GAGCCTCCTTTTCGCCAATCCATCGACAATCACCGCGAGTCCCTGCTCGAACGCTGCGTC CTCGGAGGAAAAGCGGTTAGGTAGCTGTTAGTGGCGCTCAGGGACGAGCTTGCGACGCAG CspI BstD102I 1441 CGGACCGGCTTCGTCGAAGGCGTCTATCGCGGCCCGCAACAGCGGCGAGAGCGGAGCCTG GCCTGGCCGAAGCAGCTTCCGCAGATAGCGCCGGGCGTTGTCGCCGCTCTCGCCTCGGAC NaeI NaeI Eco56I Eco56I 1501 TTCAACGGTGCCGCCGCGCTCGCCGGCATCGCTGTCGCCGGCCTGCTCCTCAAGCACGGC AAGTTGCCACGGCGGCGCGAGCGGCCGTAGCGACAGCGGCCGGACGAGGAGTTCGTGCCG Eco52I XmaIII Tth111I EagI 1561 CCCAACAGTGAAGTAGCTGATTGTCATCAGCGCATTGACGGCGTCCCCGGCCGAAAAACC GGGTTGTCACTTCATCGACTAACAGTAGTCGCGTAACTGCCGCAGGGGCCGGCTTTTTGG Eco52I EagI EcoNI XmaIII 1621 CGCCTCGCAGAGGAAGCGAAGCTGCGCGTCGGCCGTTTCCATCTGCGGTGCGCCCGGTCG GCGGAGCGTCTCCTTCGCTTCGACGCGCAGCCGGCAAAGGTAGACGCCACGCGGGCCAGC NaeI BssHII ApaBI Eco56I BssHII AlwNI 1681 CGTGCCGGCATGGATGCGCGCGCCATCGCGGTAGGCGAGCAGCGCCTGCCTGAAGCTGCG GCACGGCCGTACCTACGCGCGCGGTAGCGCCATCCGCTCGTCGCGGACGGACTTCGACGC BsmI BsmI Eco57I ApaBI 1741 GGCATTCCCGATCAGAAATGAGCGCCAGTCGTCGTCGGCTCTCGGCACCGAATGCGTATG CCGTAAGGGCTAGTCTTTACTCGCGGTCAGCAGCAGCCGAGAGCCGTGGCTTACGCATAC BglI 1801 ATTCTCCGCCAGCATGGCTTCGGCCAGTGCGTCGAGCAGCGCCCGCTTGTTCCTGAAGTG TAAGAGGCGGTCGTACCGAAGCCGGTCACGCAGCTCGTCGCGGGCGAACAAGGACTTCAC Eco57I NaeI Eco56I 1861 CCAGTAAAGCGCCGGCTGCTGAACCCCCAACCGTTCCGCCAGTTTGCGTGTCGTCAGACC GGTCATTTCGCGGCCGACGACTTGGGGGTTGGCAAGGCGGTCAAACGCACAGCAGTCTGG 1921 GTCTACGCCGACCTCGTTCAACAGGTCCAGGGCGGCACGGATCACTGTATTCGGCTGCAA CAGATGCGGCTGGAGCAAGTTGTCCAGGTCCCGCCGTGCCTAGTGACATAAGCCGACGTT 1981 CTTTGTCATGCTTGACACTTTATCACTGATAAACATAATATGTCCACCAACTTATCAGTG GAAACAGTACGAACTGTGAAATAGTGACTATTTGTATTATACAGGTGGTTGAATAGTCAC AccIII BspMII 2041 ATAAAGAATCCGCGCGTTCAATCGGACCAGCGGAGGCTGGTCCGGAGGCCAGACGTGAAA TATTTCTTAGGCGCGCAAGTTAGCCTGGTCGCCTCCGACCAGGCCTCCGGTCTGCACTTT Tth111I 2101 CCCAACATACCCCTGATCGTAATTCTGAGCACTGTCGCGCTCGACGCTGTCGGCATCGGC GGGTTGTATGGGGACTAGCATTAAGACTCGTGACAGCGCGAGCTGCGACAGCCGTAGCCG AatII 2161 CTGATTATGCCGGTGCTGCCGGGCCTCCTGCGCGATCTGGTTCACTCGAACGACGTCACC GACTAATACGGCCACGACGGCCCGGAGGACGCGCTAGACCAAGTGAGCTTGCTGCAGTGG BsmI MstI 2221 GCCCACTATGGCATTCTGCTGGCGCTGTATGCGTTGGTGCAATTTGCCTGCGCACCTGTG CGGGTGATACCGTAAGACGACCGCGACATACGCAACCACGTTAAACGGACGCGTGGACAC EheI NarI NaeI BssHII Eco56I 2281 CTGGGCGCGCTGTCGGATCGTTTCGGGCGGCGGCCAATCTTGCTCGTCTCGCTGGCCGGC GACCCGCGCGACAGCCTAGCAAAGCCCGCCGCCGGTTAGAACGAGCAGAGCGACCGGCCG BglII BbeI SphI 2341 GCCAGATCTGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGAACGGATAAACCT CGGTCTAGACCCCTTGGGACACCAACCGTACGTGTATGTTTACCTGCTTGCCTATTTGGA AhaIII DraI 2401 TTTCACGCCCTTTTAAATATCCGATTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACC AAAGTGCGGGAAAATTTATAGGCTAATAAGATTATTTGCGAGAAAAGAGAATCCAAATGG PmeI AhaIII BclI DraI Eco57I 2461 CGCCAATATATCCTGTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGA GCGGTTATATAGGACAGTTTGTGACTATCAAATTTGACTTCCGCCCTTTGCTGTTAGACT BspHI BstD102I 2521 TCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCC AGTACTCGCCTCTTAATTCCCTCAGTGCAATACTGGGGGCGGCTACTGCGCCCTGTTCGG SstII SacII AclI Mlu113I 2581 GTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTC CAAAATGCAAACCTTGACTGTCTTGGCGTTGCAACTTCCTCGGTGAGTCGGCGCCCAAAG CvnI SauI EspI MstII Bpu1102I Bsu36I 2641 TGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTCAAAAGTCGCC ACCTCAAATTACTCGATTCGTGTATGCAGTCTTTGGTAATAACGCGCAAGTTTTCAGCGG NheI SspI 2701 TAAGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAA ATTCCAGTGATAGTCGATCGTTTATAAAGAACAGTTTTTACGAGGTGACTGCAAGGTATT 2761 ATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAATCCAAATAATCTGCACCG TAAGGGGAGCCATAGGTTAATCTCAGAGTATAAGTGAGAGTTAGGTTTATTAGACGTGGC Eco52I EagI BspMI XmaIII 2821 GATCTGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTT CTAGACCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAA 2881 GGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCG CCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGC BbeI EheI NarI DrdI 2941 CCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCG GGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGC MscI PstI BalI 3001 GTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCG CACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGC PvuII MstI Tth111I Eco57I 3061 TTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGG AAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACC 3121 GCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCA CGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGT BspMI 3181 TCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACC AGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGG 3241 ACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATC TGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAG SapI 3301 AGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCA TCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGT BssHII SphI NcoI 3361 AGGCGCGCATGCCCGACGGCGATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGA TCCGCGCGTACGGGCTGCCGCTACTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCT NaeI Eco56I 3421 ATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGG TATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACC CspI SapI 3481 CGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCG GCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGC Eco57I BsiI BstD102I 3541 AATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCG TTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGC Csp45I BstD102I AsuII 3601 CCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGA GGAAGATAGCGGAAGAACTGCTCAAGAAGACTCGCCCTGAGACCCCAAGCTTTACTGGCT BspMI BsiI 3661 CCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAG GGTTCGCTGCGGGTTGGACGGTAGTGCTCTAAAGCTAAGGTGGCGGCGGAAGATACTTTC NaeI Eco56I 3721 GTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCT CAACCCGAAGCCTTAGCAAAAGGCCCTGCGGCCGACCTACTAGGAGGTCGCGCCCCTAGA EcoRV 3781 CATGCTGGAGTTCTTCGCCCACGGGATCTCTGCGGAACAGGCGGTCGAAGGTGCCGATAT GTACGACCTCAAGAAGCGGGTGCCCTAGAGACGCCTTGTCCGCCAGCTTCCACGGCTATA EagI XmaIII Eco52I 3841 CATTACGACAGCAACGGCCGACAAGCACAACGCCACGATCCTGAGCGACAATATGATCGG GTAATGCTGTCGTTGCCGGCTGTTCGTGTTGCGGTGCTAGGACTCGCTGTTATACTAGCC ApaI 3901 GCCCGGCGTCCACATCAACGGCGTCGGCGGCGACTGCCCAGGCAAGACCGAGATGCACCG CGGGCCGCAGGTGTAGTTGCCGCAGCCGCCGCTGACGGGTCCGTTCTGGCTCTACGTGGC EcoRV 3961 CGATATCTTGCTGCGTTCGGATATTTTCGTGGAGTTCCCGCCACAGACCCGGATGATCCC GCTATAGAACGACGCAAGCCTATAAAAGCACCTCAAGGGCGGTGTCTGGGCCTACTAGGG PvuI XorII AflII 4021 CGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGC GCTAGCAAGTTTGTAAACCGTTATTTCAAAGAATTCTAACTTAGGACAACGGCCAGAACG 4081 GATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATG CTACTAATAGTATATTAAAGACAACTTAATGCAATTCGTACATTATTAATTGTACATTAC NsiI 4141 CATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATA GTACTGCAATAAATACTCTACCCAAAAATACTAATCTCAGGGCGTTAATATGTAAATTAT BssHII BssHII 4201 CGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATC GCGCTATCTTTTGTTTTATATCGCGCGTTTGATCCTATTTAATAGCGCGCGCCACAGTAG 4261 TATGTTACTAGATCGGGCCTCCTGTCAATGCTGGCGGCGGCTCTGGTGGTGGTTCTGGTG ATACAATGATCTAGCCCGGAGGACAGTTACGACCGCCGCCGAGACCACCACCAAGACCAC 4321 GCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAG CGCCGAGACTCCCACCACCGAGACTCCCACCGCCAAGACTCCCACCGCCGAGACTCCCTC 4381 GCGGTTCCGGTGGTGGCTCTGGTTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTA CGCCAAGGCCACCACCGAGACCAAGGCCACTAAAACTAATACTTTTCTACCGTTTGCGAT 4441 ATAAGGGGGCTATGACCGAAAATGCCGATGAAAACGCGCTACAGTCTGACGCTAAAGGCA TATTCCCCCGATACTGGCTTTTACGGCTACTTTTGCGCGATGTCAGACTGCGATTTCCGT ClaI 4501 AACTTGATTCTGTCGCTACTGATTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTT TTGAACTAAGACAGCGATGACTAATGCCACGACGATAGCTACCAAAGTAACCACTGCAAA 4561 CCGGCCTTGCTAATGGTAATGGTGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGG GGCCGGAACGATTACCATTACCACGATGACCACTAAAACGACCGAGATTAAGGGTTTACC XmnI SspI 4621 CTCAAGTCGGTGACGGTGATAATTCACCTTTAATGAATAATTTCCGTCAATATTTACCTT GAGTTCAGCCACTGCCACTATTAAGTGGAAATTACTTATTAAAGGCAGTTATAAATGGAA 4681 CCCTCCCTCAATCGGTTGAATGTCGCCCTTTTGTCTTTGGCCCAATACGCAAACCGCCTC GGGAGGGAGTTAGCCAACTTACAGCGGGAAAACAGAAACCGGGTTATGCGTTTGGCGGAG VspI PvuII 4741 TCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAG AGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTC VspI 4801 CGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT GCCCGTCACTCGCGTTGCGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAA BstD102I 4861 TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACA ATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAGTGT SphI BspMI HindIII PstI 4921 CAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCCCCAGATTAG GTCCTTTGTCGATACTGGTACTAATGCGGTTCGAACGTACGGACGTCCAGGGGTCTAATC BspMI BsmI RleAI 4981 CCTTTTCAATTTCAGAAAGAATGCTAACCCACAGATGGTTAGAGAGGCTTACGCAGCAGG GGAAAAGTTAAAGTCTTTCTTACGATTGGGTGTCTACCAATCTCTCCGAATGCGTCGTCC Eco31I 5041 TCTCATCAAGACGATCTACCCGAGCAATAATCTCCAGGAAATCAAATACCTTCCCAAGAA AGAGTAGTTCTGCTAGATGGGCTCGTTATTAGAGGTCCTTTAGTTTATGGAAGGGTTCTT 5101 GGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATAT CCAATTTCTACGTCAGTTTTCTAAGTCCTGATTGACGTAGTTCTTGTGTCTCTTTCTATA ScaI 5161 ATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCACAAACC TAAAGAGTTCTAGTCTTCATGATAAGGTCATACCTGCTAAGTTCCGAACGAAGTGTTTGG NcoI 5221 AAGGCAAGTAATAGAGATTGGAGTCTCTAAAAAGGTAGTTCCCACTGAATCAAAGGCCAT TTCCGTTCATTATCTCTAACCTCAGAGATTTTTCCATCAAGGGTGACTTAGTTTCCGGTA 5281 GGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTT CCTCAGTTTCTAAGTTTATCTCCTGGATTGTCTTGAGCGGCATTTCTGACCGCTTGTCAA XmnI 5341 CATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCA GTATGTCTCAGAGAATGCTGAGTTACTGTTCTTCTTTTAGAAGCAGTTGTACCACCTCGT MfeI Bsc91I DrdI BbvII 5401 CGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAAT GCTGTGTGAACAGATGAGGTTTTTATAGTTTCTATGTCAGAGTCTTCTGGTTTCCCGTTA BspMII AccIII 5461 TGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTAT ACTCTGAAAAGTTGTTTCCCATTATAGGCCTTTGGAGGAGCCTAAGGTAACGGGTCGATA 5521 CTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTG GACAGTGAAATAACACTTCTATCACCTTTTCCTTCCACCGAGGATGTTTACGGTAGTAAC 5581 CGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACC GCTATTTCCTTTCCGGTAGCAACTTCTACGGAGACGGCTGTCACCAGGGTTTCTACCTGG BbvII Bsc91I BbvII Bsc91I BsiI XmnI 5641 CCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGT GGGTGGGTGCTCCTCGTAGCACCTTTTTCTTCTGCAAGGTTGGTGCAGAAGTTTCGTTCA EcoRV 5701 GGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCA CCTAACTACACTATAGAGGTGACTGCATTCCCTACTGCGTGTTAGGGTGATAGGAAGCGT XbaI 5761 AGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGAACACGGGGGACTCTAGA TCTGGGAAGGAGATATATTCCTTCAAGTAAAGTAAACCTCTCTTGTGCCCCCTGAGATCT SmaI XmaI BamHI 5821 GGATCCCCGGGTGGTCAGTCCCTTATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATC CCTAGGGGCCCACCAGTCAGGGAATACAATGCAGGACATCTTTGGGGTTGGGCACTTTAG NruI RleAI BsmI SpoI BclI 5881 AAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAG TTTTTTGAGCTGCCGGACACCCGTAAGTCAGACCTAGCGCTTTTGACACCTTAACTAGTC MfeI 5941 CGTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCTGTGCCAGGCAGTTTTAAC GCAACCACCCTTTCGCGCAATGTTCTTTCGGCCCGTTAACGACACGGTCCGTCAAAATTG 6001 GATCAGTTCGCCGATGCAGATATTCGTAATTATGCGGGCAACGTCTGGTATCAGCGCGAA CTAGTCAAGCGGCTACGTCTATAAGCATTAATACGCCCGTTGCAGACCATAGTCGCGCTT 6061 GTCTTTATACCGAAAGGTTGGGCAGGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACT CAGAAATATGGCTTTCCAACCCGTCCGGTCGCATAGCACGACGCAAAGCTACGCCAGTGA RleAI 6121 CATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCATCAGGGCGGCTATACG GTAATGCCGTTTCACACCCAGTTATTAGTCCTTCACTACCTCGTAGTCCCGCCGATATGC SnaBI 6181 CCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTT GGTAAACTTCGGCTACAGTGCGGCATACAATAACGGCCCTTTTCACATGCATAGTGGCAA 6241 TGTGTGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAA ACACACTTGTTGCTTGACTTGACCGTCTGATAGGGCGGCCCTTACCACTAATGGCTGCTT 6301 AACGGCAAGAAAAAGCAGTCTTACTTCCATGATTTCTTTAACTATGCCGGAATCCATCGC TTGCCGTTCTTTTTCGTCAGAATGAAGGTACTAAAGAAATTGATACGGCCTTAGGTAGCG EcoRV 6361 AGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATATCACCGTGGTGACGCAT TCGCATTACGAGATGTGGTGCGGCTTGTGGACCCACCTGCTATAGTGGCACCACTGCGTA MscI MluI BspMI BalI 6421 GTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATGTC CAGCGCGTTCTGACATTGGTGCGCAGACAACTGACCGTCCACCACCGGTTACCACTACAG 6481 AGCGTTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACTAGCGGG TCGCAACTTGACGCACTACGCCTAGTTGTCCACCAACGTTGACCTGTTCCGTGATCGCCC 6541 ACTTTGCAAGTGGTGAATCCGCACCTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTG TGAAACGTTCACCACTTAGGCGTGGAGACCGTTGGCCCACTTCCAATAGAGATACTTGAC EcoRV 6601 TGCGTCACAGCCAAAAGCCAGACAGAGTGTGATATCTACCCGCTTCGCGTCGGCATCCGG ACGCAGTGTCGGTTTTCGGTCTGTCTCACACTATAGATGGGCGAAGCGCAGCCGTAGGCC AlwNI 6661 TCAGTGGCAGTGAAGGGCGAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGC AGTCACCGTCACTTCCCGCTTGTCAAGGACTAATTGGTGTTTGGCAAGATGAAATGACCG ApaLI BspHI Alw44I 6721 TTTGGTCGTCATGAAGATGCGGACTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTG AAACCAGCAGTACTTCTACGCCTGAACGCACCGTTTCCTAAGCTATTGCACGACTACCAC VspI 6781 CACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCT GTGCTGGTGCGTAATTACCTGACCTAACCCCGGTTGAGGATGGCATGGAGCGTAATGGGA Eco57I 6841 TACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGATTGATGAAACT ATGCGACTTCTCTACGAGCTGACCCGTCTACTTGTACCGTAGCACCACTAACTACTTTGA Csp45I AsuII 6901 GCTGCTGTCGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAA CGACGACAGCCGAAATTGGAGAGAAATCCGTAACCAAAGCTTCGCCCGTTGTTCGGCTTT Bsp1407I 6961 GAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATT CTTGACATGTCGCTTCTCCGTCAGTTGCCCCTTTGAGTCGTTCGCGTGAATGTCCGCTAA 7021 AAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAAC TTTCTCGACTATCGCGCACTGTTTTTGGTGGGTTCGCACCACTACACCTCATAACGGTTG Alw44I ApaLI SspI MluI 7081 GAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTGGCGGAAGCAACG CTTGGCCTATGGGCAGGCGTTCCACGTGCCCTTATAAAGCGCGGTGACCGCCTTCGTTGC MluI BspMI 7141 CGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCAC GCATTTGAGCTGGGCTGCGCAGGCTAGTGGACGCAGTTACATTACAAGACGCTGCGAGTG 7201 ACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTAT TGGCTATGGTAGTCGCTAGAGAAACTACACGACACGGACTTGGCAATAATGCCTACCATA 7261 GTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGG CAGGTTTCGCCGCTAAACCTTTGCCGTCTCTTCCATGACCTTTTTCTTGAAGACCGGACC 7321 CAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGTGGATACGTTAGCCGGG GTCCTCTTTGACGTAGTCGGCTAATAGTAGTGGCTTATGCCGCACCTATGCAATCGGCCC Bsp1407I 7381 CTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATG GACGTGAGTTACATGTGGCTGTACACCTCACTTCTCATAGTCACACGTACCGACCTATAC 7441 TATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCC ATAGTGGCGCAGAAACTAGCGCAGTCGCGGCAGCAGCCACTTGTCCATACCTTAAAGCGG 7501 GATTTTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACT CTAAAACGCTGGAGCGTTCCGTATAACGCGCAACCGCCATTGTTCTTTCCCTAGAAGTGA SpoI NruI 7561 CGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAAC GCGCTGGCGTTTGGCTTCAGCCGCCGAAAAGACGACGTTTTTGCGACCTGACCGTACTTG 7621 TTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGAATCAACAACTCTCCTGGCGCACCA AAGCCACTTTTTGGCGTCGTCCCTCCGTTTGTTACTTAGTTGTTGAGAGGACCGCGTGGT SacI SstI Ecl136II XorII EcoICRI PvuI 7681 TCGTCGGCTACAGCCTCGGGAATTGCTACCGAGCTCGAATTTCCCCGATCGTTCAAACAT AGCAGCCGATGTCGGAGCCCTTAACGATGGCTCGAGCTTAAAGGGGCTAGCAAGTTTGTA AflII 7741 TTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATA AACCGTTATTTCAAAGAATTCTAACTTAGGACAACGGCCAGAACGCTACTAATAGTATAT NsiI 7801 ATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTAT TAAAGACAACTTAATGCAATTCGTACATTATTAATTGTACATTACGTACTGCAATAAATA 7861 GAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAA CTCTACCCAAAAATACTAATCTCAGGGCGTTAATATGTAAATTATGCGCTATCTTTTGTT BssHII BssHII 7921 AATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCG TTATATCGCGCGTTTGATCCTATTTAATAGCGCGCGCCACAGTAGATACAATGATCTAGC EcoRI 7981 GGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAAC CCTTAAGTGACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTG PvuII 8041 TTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCA AATTAGCGGAACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGT BstD102I BbeI EheI XorII NarI PvuI MstI BglI 8101 CCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGCCCGCTCCTTTCGCTTTCTTC GGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCGGGCGAGGAAAGCGAAAGAAG NaeI Eco56I 8161 CCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCT GGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGA 8221 TTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGAT AATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAAACCCACTA DraIII DrdI 8281 GGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCC CCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGG 8341 ACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGC TGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCCG 8401 TATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGAACCACCATCAAACAGGATTTTC ATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCTTGGTGGTAGTTTGTCCTAAAAG 8461 GCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGA CGGACGACCCCGTTTGGTCGCACCTGGCGAACGACGTTGAGAGAGTCCCGGTCCGCCACT PvuII 8521 AGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAA TCCCGTTAGTCGACAACGGGCAGAGTGACCACTTTTCTTTTTGGTGGGGTCATGTAATTT 8581 AACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATC TTGCAGGCGTTACACAATAATTCAACAGATTCGCAGTTAAACAAATGTGGTGTTATATAG 8641 CTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCAGCTCGGCACAAAATCACCACTCGAT GACGGTGGTCGGTCGGTTGTCGAGGGGCTGGCCGTCGAGCCGTGTTTTAGTGGTGAGCTA Tth111I 8701 ACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGTTGTAAGGCGGCAGACT TGTCCGTCGGGTAGTCAGGCCCTGCCGCAGTCGCCCTCTCGGCAACATTCCGCCGTCTGA ApaBI 8761 TTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCTGCCGGGTTTGAAACAC AACGAGTACAATGGCTACGATAAGCCTTCTTGCCGTTGATTCGACGGCCCAAACTTTGTG BclI 8821 GGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAGCGTTGCTGCCTGTG CCTACTAGAGCGCCTCCCATCGTACAACTAACATTGCTACTGTCTCGCAACGACGGACAC 8881 ATCAAATATCATCTCCCTCGCAGAGATCCGAATTATCAGCCTTCTTATTCATTTCTCGCT TAGTTTATAGTAGAGGGAGCGTCTCTAGGCTTAATAGTCGGAAGAATAAGTAAAGAGCGA Eam1105I 8941 TAACCGTGACAGGCTGTCGATCTTGAGAACTATGCCGACATAATAGGAAATCGCTGGATA ATTGGCACTGTCCGACAGCTAGAACTCTTGATACGGCTGTATTATCCTTTAGCGACCTAT AclI EcoRV 9001 AAGCCGCTGAGGAAGCTGAGTGGCGCTATTTCTTTAGAAGTGAACGTTGACGATATCAAC TTCGGCGACTCCTTCGACTCACCGCGATAAAGAAATCTTCACTTGCAACTGCTATAGTTG 9061 TCCCCTATCCATTGCTCACCGAATGGTACAGGTCGGGGACCCGAAGTTCCGACTGTCGGC AGGGGATAGGTAACGAGTGGCTTACCATGTCCAGCCCCTGGGCTTCAAGGCTGACAGCCG NsiI BglII 9121 CTGATGCATCCCCGGCTGATCGACCCCAGATCTGGGGCTGAGAAAGCCCAGTAAGGAAAC GACTACGTAGGGGCCGACTAGCTGGGGTCTAGACCCCGACTCTTTCGGGTCATTCCTTTG NruI SpoI BstD102I 9181 AACTGTAGGTTCGAGTCGCGAGATCCCCCGGAACCAAAGGAAGTAGGTTAAACCCGCTCC TTGACATCCAAGCTCAGCGCTCTAGGGGGCCTTGGTTTCCTTCATCCAATTTGGGCGAGG 9241 GATCAGGCCGAGCCACGCCAGGCCGAGAACATTGGTTCCTGTAGGCATCGGGATTGGCGG CTAGTCCGGCTCGGTGCGGTCCGGCTCTTGTAACCAAGGACATCCGTAGCCCTAACCGCC EagI XmaIII Eco52I 9301 ATCAAACACTAAAGCTACTGGAACGAGCAGAAGTCCTCCGGCCGCCAGTTGCCAGGCGGT TAGTTTGTGATTTCGATGACCTTGCTCGTCTTCAGGAGGCCGGCGGTCAACGGTCCGCCA NcoI 9361 AAAGGTGAGCAGAGGCACGGGAGGTTGCCACTTGCGGGTCAGCACGGTTCCGAACGCCAT TTTCCACTCGTCTCCGTGCCCTCCAACGGTGAACGCCCAGTCGTGCCAAGGCTTGCGGTA Eco47III 9421 GGAAACCGCCCCCGCCAGGCCCGCTGCGACGCCGACAGGATCTAGCGCTGCGTTTGGTGT CCTTTGGCGGGGGCGGTCCGGGCGACGCTGCGGCTGTCCTAGATCGCGACGCAAACCACA 9481 CAACACCAACAGCGCCACGCCCGCAGTTCCGCAAATAGCCCCCAGGACCGCCATCAATCG GTTGTGGTTGTCGCGGTGCGGGCGTCAAGGCGTTTATCGGGGGTCCTGGCGGTAGTTAGC BstD102I AlwNI 9541 TATCGGGCTACCTAGCAGAGCGGCAGAGATGAACACGACCATCAGCGGCTGCACAGCGCC ATAGCCCGATGGATCGTCTCGCCGTCTCTACTTGTGCTGGTAGTCGCCGACGTGTCGCGG BglI 9601 TACCGTCGCCGCGACCCCGCCCGGCAGGCGGTAGACCGAAATAAACAACAAGCTCCAGAA ATGGCAGCGGCGCTGGGGCGGGCCGTCCGCCATCTGGCTTTATTTGTTGTTCGAGGTCTT SspI MstI XcmI 9661 TAGCGAAATATTAAGTGCGCCGAGGATGAAGATGCGCATCCACCAGATTCCCGTTGGAAT ATCGCTTTATAATTCACGCGGCTCCTACTTCTACGCGTAGGTGGTCTAAGGGCAACCTTA NaeI BsiI Eco56I 9721 CTGTCGGACGATCATCACGAGCAATAAACCCGCCGGCAACGCCCGCAGCAGCATACCGGC GACAGCCTGCTAGTAGTGCTCGTTATTTGGGCGGCCGTTGCGGGCGTCGTCGTATGGCCG 9781 GACCCCTCGGCCTCGCTGTTCGGGCTCCACGAAAACGCCGGACAGATGCGCCTTGTGAGC CTGGGGAGCCGGAGCGACAAGCCCGAGGTGCTTTTGCGGCCTGTCTACGCGGAACACTCG Bsc91I BbvII 9841 GTCCTTGGGGCCGTCCTCCTGTTTGAAGACCGACAGCCCAATGATCTCGCCGTCGATGTA CAGGAACCCCGGCAGGAGGACAAACTTCTGGCTGTCGGGTTACTAGAGCGGCAGCTACAT BbeI EheI NarI BsmI NcoI 9901 GGCGCCGAATGCCACGGCATCTCGCAACCGTTCAGCGAACGCCTCCATGGGCTTTTTCTC CCGCGGCTTACGGTGCCGTAGAGCGTTGGCAAGTCGCTTGCGGAGGTACCCGAAAAAGAG BsiI Eco57I 9961 CTCGTGCTCGTAAACGGACCCGAACATCTCTGGAGCTTTCTTCAGGGCCGACAATCGGAT GAGCACGAGCATTTGCCTGGGCTTGTAGAGACCTCGAAAGAAGTCCCGGCTGTTAGCCTA BstD102I Eco52I XmaIII EagI MfeI 10021 CTCGCGGAAATCCTGCACGTCGGCCGCTCCAAGCCGTCGAATCTGAGCCTTAATCACAAT GAGCGCCTTTAGGACGTGCAGCCGGCGAGGTTCGGCAGCTTAGACTCGGAATTAGTGTTA BssHII 10081 TGTCAATTTTAATCCTCTGTTTATCGGCAGTTCGTAGAGCGCGCCGTGCGTCCCGAGCGA ACAGTTAAAATTAGGAGACAAATAGCCGTCAAGCATCTCGCGCGGCACGCAGGGCTCGCT Eco47III XcmI 10141 TACTGAGCGAAGCAAGTGCGTCGAGCAGTGCCCGCTTGTTCCTGAAATGCCAGTAAAGCG ATGACTCGCTTCGTTCACGCAGCTCGTCACGGGCGAACAAGGACTTTACGGTCATTTCGC RleAI 10201 CTGGCTGCTGAACCCCCAGCCGGAACTGACCCCACAAGGCCCTAGCGTTTGCAATGCACC GACCGACGACTTGGGGGTCGGCCTTGACTGGGGTGTTCCGGGATCGCAAACGTTACGTGG 10261 AGGTCATCATTGACCCAGGCGTGTTCCACCAGGCCGCTGCCTCGCAACTCTTCGCAGGCT TCCAGTAGTAACTGGGTCCGCACAAGGTGGTCCGGCGACGGAGCGTTGAGAAGCGTCCGA BspMI 10321 TCGCCGACCTGCTCGCGCCACTTCTTCACGCGGGTGGAATCCGATCCGCACATGAGGCGG AGCGGCTGGACGAGCGCGGTGAAGAAGTGCGCCCACCTTAGGCTAGGCGTGTACTCCGCC BstD102I 10381 AAGGTTTCCAGCTTGAGCGGGTACGGCTCCCGGTGCGAGCTGAAATAGTCGAACATCCGT TTCCAAAGGTCGAACTCGCCCATGCCGAGGGCCACGCTCGACTTTATCAGCTTGTAGGCA NdeI 10441 CGGGCCGTCGGCGACAGCTTGCGGTACTTCTCCCATATGAATTTCGTGTAGTGGTCGCCA GCCCGGCAGCCGCTGTCGAACGCCATGAAGAGGGTATACTTAAAGCACATCACCAGCGGT AlwNI 10501 GCAAACAGCACGACGATTTCCTCGTCGATCAGGACCTGGCAACGGGACGTTTTCTTGCCA CGTTTGTCGTGCTGCTAAAGGAGCAGCTAGTCCTGGACCGTTGCCCTGCAAAAGAACGGT 10561 CGGTCCAGGACGCGGAAGCGGTGCAGCAGCGACACCGATTCCAGGTGCCCAACGCGGTCG GCCAGGTCCTGCGCCTTCGCCACGTCGTCGCTGTGGCTAAGGTCCACGGGTTGCGCCAGC BsmI 10621 GACGTGAAGCCCATCGCCGTCGCCTGTAGGCGCGACAGGCATTCCTCGGCCTTCGTGTAA CTGCACTTCGGGTAGCGGCAGCGGACATCCGCGCTGTCCGTAAGGAGCCGGAAGCACATT PflMI AlwNI 10681 TACCGGCCATTGATCGACCAGCCCAGGTCCTGGCAAAGCTCGTAGAACGTGAAGGTGATC ATGGCCGGTAACTAGCTGGTCGGGTCCAGGACCGTTTCGAGCATCTTGCACTTCCACTAG BspMI 10741 GGCTCGCCGATAGGGGTGCGCTTCGCGTACTCCAACACCTGCTGCCACACCAGTTCGTCA CCGAGCGGCTATCCCCACGCGAAGCGCATGAGGTTGTGGACGACGGTGTGGTCAAGCAGT SgrAI 10801 TCGTCGGCCCGCAGCTCGACGCCGGTGTAGGTGATCTTCACGTCCTTGTTGACGTGGAAA AGCAGCCGGGCGTCGAGCTGCGGCCACATCCACTAGAAGTGCAGGAACAACTGCACCTTT 10861 ATGACCTTGTTTTGCAGCGCCTCGCGCGGGATTTTCTTGTTGCGCGTGGTGAACAGGGCA TACTGGAACAAAACGTCGCGGAGCGCGCCCTAAAAGAACAACGCGCACCACTTGTCCCGT BstD102I 10921 GAGCGGGCCGTGTCGTTTGGCATCGCTCGCATCGTGTCCGGCCACGGCGCAATATCGAAC CTCGCCCGGCACAGCAAACCGTAGCGAGCGTAGCACAGGCCGGTGCCGCGTTATAGCTTG BglI SfiI 10981 AAGGAAAGCTGCATTTCCTTGATCTGCTGCTTCGTGTGTTTCAGCAACGCGGCCTGCTTG TTCCTTTCGACGTAAAGGAACTAGACGACGAAGCACACAAAGTCGTTGCGCCGGACGAAC NaeI Eco56I SgrAI 11041 GCCTCGCTGACCTGTTTTGCCAGGTCCTCGCCGGCGGTTTTTCGCTTCTTGGTCGTCATA CGGAGCGACTGGACAAAACGGTCCAGGAGCGGCCGCCAAAAAGCGAAGAACCAGCAGTAT BspMI 11101 GTTCCTCGCGTGTCGATGGTCATCGACTTCGCCAAACCTGCCGCCTCCTGTTCGAGACGA CAAGGAGCGCACAGCTACCAGTAGCTGAAGCGGTTTGGACGGCGGAGGACAAGCTCTGCT EagI XmaIII Eco52I 11161 CGCGAACGCTCCACGGCGGCCGATGGCGCGGGCAGGGCAGGGGGAGCCAGTTGCACGCTG GCGCTTGCGAGGTGCCGCCGGCTACCGCGCCCGTCCCGTCCCCCTCGGTCAACGTGCGAC 11221 TCGCGCTCGATCTTGGCCGTAGCTTGCTGGACCATCGAGCCGACGGACTGGAAGGTTTCG AGCGCGAGCTAGAACCGGCATCGAACGACCTGGTAGCTCGGCTGCCTGACCTTCCAAAGC 11281 CGGGGCGCACGCATGACGGTGCGGCTTGCGATGGTTTCGGCATCCTCGGCGGAAAACCCC GCCCCGCGTGCGTACTGCCACGCCGAACGCTACCAAAGCCGTAGGAGCCGCCTTTTGGGG 11341 GCGTCGATCAGTTCTTGCCTGTATGCCTTCCGGTCAAACGTCCGATTCATTCACCCTCCT CGCAGCTAGTCAAGAACGGACATACGGAAGGCCAGTTTGCAGGCTAAGTAAGTGGGAGGA 11401 TGCGGGATTGCCCCGACTCACGCCGGGGCAATGTGCCCTTATTCCTGATTTGACCCGCCT ACGCCCTAACGGGGCTGAGTGCGGCCCCGTTACACGGGAATAAGGACTAAACTGGGCGGA 11461 GGTGCCTTGGTGTCCAGATAATCCACCTTATCGGCAATGAAGTCGGTCCCGTAGACCGTC CCACGGAACCACAGGTCTATTAGGTGGAATAGCCGTTACTTCAGCCAGGGCATCTGGCAG 11521 TGGCCGTCCTTCTCGTACTTGGTATTCCGAATCTTGCCCTGCACGAATACCAGCGACCCC ACCGGCAGGAAGAGCATGAACCATAAGGCTTAGAACGGGACGTGCTTATGGTCGCTGGGG 11581 TTGCCCAAATACTTGCCGTGGGCCTCGGCCTGAGAGCCAAAACACTTGATGCGGAAGAAG AACGGGTTTATGAACGGCACCCGGAGCCGGACTCTCGGTTTTGTGAACTACGCCTTCTTC NaeI Eco56I 11641 TCGGTGCGCTCCTGCTTGTCGCCGGCATCGTTGCGCCACATCTAGGTACTAAAACAATTC AGCCACGCGAGGACGAACAGCGGCCGTAGCAACGCGGTGTAGATCCATGATTTTGTTAAG SspI 11701 ATCCAGTAAAATATAATATTTTATTTTCTCCCAATCAGGCTTGATCCCCAGTAAGTCAAA TAGGTCATTTTATATTATAAAATAAAAGAGGGTTAGTCCGAACTAGGGGTCATTCAGTTT EcoRV 11761 AAATAGCTCGACATACTGTTCTTCCCCGATATCCTCCCTGATCGACCGGACGCAGAAGGC TTTATCGAGCTGTATGACAAGAAGGGGCTATAGGAGGGACTAGCTGGCCTGCGTCTTCCG 11821 AATGTCATACCACTTGTCCGCCCTGCCGCTTCTCCCAAGATCAATAAAGCCACTTACTTT TTACAGTATGGTGAACAGGCGGGACGGCGAAGAGGGTTCTAGTTATTTCGGTGAATGAAA 11881 GCCATCTTTCACAAAGATGTTGCTGTCTCCCAGGTCGCCGTGGGAAAAGACAAGTTCCTC CGGTAGAAAGTGTTTCTACAACGACAGAGGGTCCAGCGGCACCCTTTTCTGTTCAAGGAG Bsc91I BbvII AhaIII AhaIII DraI DraI 11941 TTCGGGCTTTTCCGTCTTTAAAAAATCATACAGCTCGCGCGGATCTTTAAATGGAGTGTC AAGCCCGAAAAGGCAGAAATTTTTTAGTATGTCGAGCGCGCCTAGAAATTTACCTCACAG 12001 TTCTTCCCAGTTTTCGCAATCCACATCGGCCAGATCGTTATTCAGTAAGTAATCCAATTC AAGAAGGGTCAAAAGCGTTAGGTGTAGCCGGTCTAGCAATAAGTCATTCATTAGGTTAAG EspI Bpu1102I 12061 GGCTAAGCGGCTGTCTAAGCTATTCGTATAGGGACAATCCGATATGTCGATGGAGTGAAA CCGATTCGCCGACAGATTCGATAAGCATATCCCTGTTAGGCTATACAGCTACCTCACTTT 12121 GAGCCTGATGCACTCCGCATACAGCTCGATAATCTTTTCAGGGCTTTGTTCATCTTCATA CTCGGACTACGTGAGGCGTATGTCGAGCTATTAGAAAAGTCCCGAAACAAGTAGAAGTAT BspHI 12181 CTCTTCCGAGCAAAGGACGCCATCGGCCTCACTCATGAGCAGATTGCTCCAGCCATCATG GAGAAGGCTCGTTTCCTGCGGTAGCCGGAGTGAGTACTCGTCTAACGAGGTCGGTAGTAC 12241 CCGTTCAAAGTGCAGGACCTTTGGAACAGGCAGCTTTCCTTCCAGCCATAGCATCATGTC GGCAAGTTTCACGTCCTGGAAACCTTGTCCGTCGAAAGGAAGGTCGGTATCGTAGTACAG DraI XcmI AhaIII 12301 CTTTTCCCGTTCCACATCATAGGTGGTCCCTTTATACCGGCTGTCCGTCATTTTTAAATA GAAAAGGGCAAGGTGTAGTATCCACCAGGGAAATATGGCCGACAGGCAGTAAAAATTTAT 12361 TAGGTTTTCATTTTCTCCCACCAGCTTATATACCTTAGCAGGAGACATTCCTTCCGTATC ATCCAAAAGTAAAAGAGGGTGGTCGAATATATGGAATCGTCCTCTGTAAGGAAGGCATAG 12421 TTTTACGCAGCGGTATTTTTCGATCAGTTTTTTCAATTCCGGTGATATTCTCATTTTAGC AAAATGCGTCGCCATAAAAAGCTAGTCAAAAAAGTTAAGGCCACTATAAGAGTAAAATCG DraI AhaIII 12481 CATTTATTATTTCCTTCCTCTTTTCTACAGTATTTAAAGATACCCCAAGAAGCTAATTAT GTAAATAATAAAGGAAGGAGAAAAGATGTCATAAATTTCTATGGGGTTCTTCGATTAATA BsmI 12541 AACAAGACGAACTCCAATTCACTGTTCCTTGCATTCTAAAACCTTAAATACCAGAAAACA TTGTTCTGCTTGAGGTTAAGTGACAAGGAACGTAAGATTTTGGAATTTATGGTCTTTTGT 12601 GCTTTTTCAAAGTTGTTTTCAAAGTTGGCGTATAACATAGTATCGACGGAGCCGATTTTG CGAAAAAGTTTCAACAAAAGTTTCAACCGCATATTGTATCATAGCTGCCTCGGCTAAAAC BstXI 12661 AAACCACAATTATGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGA TTTGGTGTTAATACCCACTACGACGGTTGAATGACTAAATCACATACTACCACAAAAACT PvuII AlwNI 12721 GGTGCTCCAGTGGCTTCTGTGTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTG CCACGAGGTCACCGAAGACACAGATAGTCGACAGGGAGGACAAGTCGATGACTGCCCCAC Eco47III 12781 GTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAA CACGCATTGCCGTTTTCGTGGCGGCCTGTAGTCGCGATAGAGACGAGAGTGACGGCATTT PstI 12841 ACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATT TGTACCGTTGACGTCAAGTGAATGTGGCGAAGAGTTGGGCCATGCGTGGTCTTTTAGTAA BalI MscI 12901 GATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTC CTATACCGGTACTTACCGCAACCTACGGCCCGTTGTCGGGCGTAATACCCGCAACCGGAG BstEII 12961 AACACGATTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGTAACCTCGCGCATACAGC TTGTGCTAAAATGCAGTGAATTTTTTGAGTCCGGCGTCAGCCATTGGAGCGCGTATGTCG DrdI AatII 13021 CGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGCTATGTCGGGGCTA GCCCGTCACTGCAGTAGCAGACGCGCCTTTACCTGCTTGTCACCCCGATACAGCCCCGAT BspMII Bsc91I Eco47III MluI AccIII BbvII MstI 13081 AATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGTCTCCGGAAGACGGTTGTTGCGC TTAGCGCGGTCGCGACCGACAAAATGCGCATACTGTCAGAGGCCTTCTGCCAACAACGCG 13141 ACGTATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATGAGCCTGCTGTCACCCT TGCATAAGCCACTTGCGTGATACCGCTGCGACCCCGCAGAATACTCGGACGACAGTGGGA 13201 TTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAA AACTGCACCACTATACCTACTGCCTACCGACCGGCGACATACTTAGGGCGGACTTCCCTT Eco57I BstD102I 13261 AGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGA TCGACGTGCATTAGTCGTTCGCTATATGCGTCGCTTAACTCGCCGTATTGGACTTAGACT AlwNI 13321 GGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGC CCGTCGTGGACCGTGCCGACCCTGCCTTCAGCGACAGCAAGAGTTTTAGCCACCTCGACG Tth111I 13381 ATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTA TACTGTTTCAGTAGCCCGTAATAGACTTGTATTTTGTGATAGTTATTCAACCTCAGTAAT 13441 CCCAATTATGATAGAATTTACAAGCTATAAGGTTATTGTCCTGGGTTTCAAGCATTAGTC GGGTTAATACTATCTTAAATGTTCGATATTCCAATAACAGGACCCAAAGTTCGTAATCAG BssHII 13501 CATGCAAGTTTTTATGCTTTGCCCATTCTATAGATATATTGATAAGCGCGCTGCCTATGC GTACGTTCAAAAATACGAAACGGGTAAGATATCTATATAACTATTCGCGCGACGGATACG EcoRV 13561 CTTGCCCCCTGAAATCCTTACATACGGCGATATCTTCTATATAAAAGATATATTATCTTA GAACGGGGGACTTTAGGAATGTATGCCGCTATAGAAGATATATTTTCTATATAATAGAAT 13621 TCAGTATTGTCAATATATTCAAGGCAATCTGCCTCCTCATCCTCTTCATCCTCTTCGTCT AGTCATAACAGTTATATAAGTTCCGTTAGACGGAGGAGTAGGAGAAGTAGGAGAAGCAGA AhaIII DraI 13681 TGGTAGCTTTTTAAATATGGCGCTTCATAGAGTAATTCTGTAAAGGTCCAATTCTCGTTT ACCATCGAAAAATTTATACCGCGAAGTATCTCATTAAGACATTTCCAGGTTAAGAGCAAA 13741 TCATACCTCGGTATAATCTTACCTATCACCTCAAATGGTTCGCTGGGTTTATCGCACCCC AGTATGGAGCCATATTAGAATGGATAGTGGAGTTTACCAAGCGACCCAAATAGCGTGGGG NaeI BsiI BsmI Eco56I 13801 CGAACACGAGCACGGCACCCGCGACCACTATGCCAAGAATGCCCAAGGTAAAAATTGCCG GCTTGTGCTCGTGCCGTGGGCGCTGGTGATACGGTTCTTACGGGTTCCATTTTTAACGGC Eco52I XmaIII EagI BglI BsmI BglI 13861 GCCCCGCCATGAAGTCCGTGAATGCCCCGACGGCCGAAGTGAAGGGCAGGCCGCCACCCA CGGGGCGGTACTTCAGGCACTTACGGGGCTGCCGGCTTCACTTCCCGTCCGGCGGTGGGT AlwNI BspMI 13921 GGCCGCCGCCCTCACTGCCCGGCACCTGGTCGCTGAATGTCGATGCCAGCACCTGCGGCA CCGGCGGCGGGAGTGACGGGCCGTGGACCAGCGACTTACAGCTACGGTCGTGGACGCCGT 13981 CGTCAATGCTTCCGGGCGTCGCGCTCGGGCTGATCGCCCATCCCGTTACTGCCCCGATCC GCAGTTACGAAGGCCCGCAGCGCGAGCCCGACTAGCGGGTAGGGCAATGACGGGGCTAGG Eco52I XmaIII ApaBI Eco47III EagI 14041 CGGCAATGGCAAGGACTGCCAGCGCTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCGAGG GCCGTTACCGTTCCTGACGGTCGCGACGGTAAAAACCCCACTCCGGCAAGCGCCGGCTCC AlwNI 14101 GGCGCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGGGAGGGTTCGAGAAGGG CCGCGTCGGGGACCCCCCTACCCTCCGGGCGCAATCGCCCGGCCCTCCCAAGCTCTTCCC 14161 GGGGCACCCCCCTTCGGCGTGCGCGGTCACGCGCACAGGGCGCAGCCCTGGTTAAAAACA CCCCGTGGGGGGAAGCCGCACGCGCCAGTGCGCGTGTCCCGCGTCGGGACCAATTTTTGT DraI AhaIII BspMI SspI 14221 AGGTTTATAAATATTGGTTTAAAAGCAGGTTAAAAGACAGGTTAGCGGTGGCCGAAAAAC TCCAAATATTTATAACCAAATTTTCGTCCAATTTTCTGTCCAATCGCCACCGGCTTTTTG 14281 GGGCGGAAACCCTTGCAAATGCTGGATTTTCTGCCTGTGGACAGCCCCTCAAATGTCAAT CCCGCCTTTGGGAACGTTTACGACCTAAAAGACGGACACCTGTCGGGGAGTTTACAGTTA 14341 AGGTGCGCCCCTCATCTGTCAGCACTCTGCCCCTCAAGTGTCAAGGATCGCGCCCCTCAT TCCACGCGGGGAGTAGACAGTCGTGAGACGGGGAGTTCACAGTTCCTAGCGCGGGGAGTA RleAI 14401 CTGTCAGTAGTCGCGCCCCTCAAGTGTCAATACCGCAGGGCACTTATCCCCAGGCTTGTC GACAGTCATCAGCGCGGGGAGTTCACAGTTATGGCGTCCCGTGAATAGGGGTCCGAACAG MscI XcmI BalI 14461 CACATCATCTGTGGGAAACTCGCGTAAAATCAGGCGTTTTCGCCGATTTGCGAGGCTGGC GTGTAGTAGACACCCTTTGAGCGCATTTTAGTCCGCAAAAGCGGCTAAACGCTCCGACCG Eco52I XmaIII EagI NaeI Eco56I 14521 CAGCTCCACGTCGCCGGCCGAAATCGAGCCTGCCCCTCATCTGTCAACGCCGCGCCGGGT GTCGAGGTGCAGCGGCCGGCTTTAGCTCGGACGGGGAGTAGACAGTTGCGGCGCGGCCCA 14581 GAGTCGGCCCCTCAAGTGTCAACGTCCGCCCCTCATCTGTCAGTGAGGGCCAAGTTTTCC CTCAGCCGGGGAGTTCACAGTTGCAGGCGGGGAGTAGACAGTCACTCCCGGTTCAAAAGG SstII SacII Mlu113I EagI Eco52I XmaIII NotI NaeI SgrAI Eco56I 14641 GCGAGGTATCCACAACGCCGGCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCT CGCTCCATAGGTGTTGCGGCCGCCGGCGCCACAGAGCGTGTGCCGAAGCTGCCGCAAAGA XmaIII Eco52I EagI 14701 GGCGCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAGCCCGG CCGCGCAAACGTCCCGGTATCTGCCGGCGGTCGGGTCGCCGCTCCCGTTGGTCGGGCC ----------------------------------------------------------------------------------------------------------------------