【精品】大鼠无血预充体外循环伴心脏停跳模型的建立

【精品】大鼠无血预充体外循环伴心脏停跳模型的建立 大鼠无血预充体外循环伴心脏停跳模型 的建立 [真诚为您服务] 作者:宋丹丹，高光洁，孙莹杰，张铁铮，周锦，张瑜，姚婧 【摘要】 目的 摸索建立大鼠无血预充体外循环伴心脏停跳模型的实验。 方 法 以雄性SD大鼠为实验动物，经右颈静脉引流、右股动脉灌注建立体外循环模 型。采用6%羟乙基淀粉进行无血预充，经右颈动脉穿刺置入冷灌管至主动脉根 部，正中劈胸骨，阻断主动脉，灌注心脏停搏液使心脏停跳，15 min后开放主动 脉，心脏自主复跳，体外循环1 h后停转流。停转流2 h后大鼠血压心率稳定视 为模型成功。结果 模型死亡率5%(2/40只)。心脏自主复跳成功率为 94.7%(36/38只)。34只顺利停转流，模型成功率85%。结论 本实验建立的无 血预充大鼠体外循环伴心脏停跳模型，操作简单，费用低廉，成功率较高，是研究 体外循环期间全身炎症反应、脏器保护机制及评估相关保护措施的良好实验工具。 【关键词】 大鼠;体外循环;心脏停跳;无血预充;动物模型 Establishment of a Rat Model of Cardiopulmonary Bypass withCardioplegic Arrest Primed without Blood Abstract: OBJECTIVE To establish a rat model of the cardiopulmonary bypass (CPB) with cardioplegic arrest primed without blood. METHODS Adult male Sprague-Dawley rats (400,500g, n = 40) were used for all experiments. Blood was drained from the right atrium via jugular vein catheter into the CPB circuit and return to the right femoral artery. The miniature cardiopulmonary bypass was primed with 6% hetastarch without blood. A catheter through the right carotid artery was inserted into the aortic root for administration of cardioplegic solution. After median sternotomy aortic arch was clamped. Cardioplegic arrest was accomplished by a cardioplegic solution. After 15 min of cardioplegia, the aortic cross-clamping was released. CPB was weaned after 1 hr. It is a validation criteria of the animal model that the arterial blood pressure and heart rate of rats were stable at 2 hrs post CPB. RESULTS Mortality rate was 5 percent. The rate of heart spontaneous re-beating was 94.7 percent. Blood pressure and heart rate were stable in 34 of 40 rats at 2 hrs post CPB. Success rate of model was 85 percent. CONCLUSION The rat model of CPB with cardioplegic arrest primed without blood is easy to be performed with low cost and higher success rate. This model is suitable for the investigation of the pathophysiological changes concerning CPB-related multiple organ dysfunction and possible protective interventions. Key words: Rat; Cardiopulmonary Bypass; Cardioplegic arrest; Primed without Blood; Animal Models 体外循环(cardiopulmonary bypass，CPB)可引起全身性炎症反应、心 肺缺血再灌注损伤，严重影响心血管手术成功及预后，一直是心脏外科关注的热 点。以往研究多应用并行循环建立大鼠CPB模型[1-3]，CPB期间心脏无停跳复 跳过程，与临床实际有很大差距。本实验旨在建立一种简单实用、价格便宜的大鼠 CPB伴心脏停跳模型，为研究CPB期间脏器损伤、全身炎症反应机制及评估相关 保护措施提供实验工具。 1 材料与方法 1.1 实验材料 健康成年雄性清洁级SD大鼠40只，体重400,500 g，由 沈阳军区总医院动物实验科提供。小动物呼吸机(江西省特力麻醉呼吸设备公司， TKR-200C型)，恒流蠕动泵(保定兰格恒流泵有限公司，BT00-300M型)，大鼠 专用膜式氧合器(面积0.05 m2，预充液3 ml，广东科威医疗器械有限公 司)，配套硅胶泵管(内径4 mm)及管道，多功能监测仪(日本光电公司)，全自动 温血仪(美国BIGGER公司)，心脏停搏液(冷灌液):HTK溶液(商品名:康斯特 HTK溶液， 德国克勒化学制药)，一次性静脉套管针(美国BD公司)。