龙牡壮骨冲剂对胃肠动力及胃肠组织VIP的影响

龙牡壮骨冲剂对胃肠动力及胃肠组织VIP的影响 龙牡壮骨冲剂对胃肠动力及胃肠组织VIP 的影响 VIP?????? ???L???????????????Y???? ???6?V?????????????(430070 ????:[????]?l?????????????????????????B???5?g?????????????????g??????mI[????]?0?5?g???v?0?v??????mV?M????mV?? ?????????B?d?B????mV?E?B????mV?5?B????,?r???)??mV?k????mV???????<mV???]???g???4???y???[???4?)?b?????????]???5?g?M ??,???G???d?%??????????,?7???????#?????????????5????????,?(?????y?????#?????????E?N???T?d????(VIP)?Y??mI[?? ??]???)???????????????L,?(???????????????EVIP?Y??????(?/?vP<005)mI?k???????????????????????????????EVIP ?Y???/??(?/?vP<005)mI???????<?????5?????????????????????????EVIP?Y??????(?/?vP<005)mI???]???g?????5?? ?????????????????????????EVIP?Y???/??(?/?vP<005)mI???????????B?????W???5?g???????????5??????????????,?d ?B?????????????B?????)???N???????????LmV?k?????N????????????mV???????<?N???5????????????mV???]???g?N???5?????? ???????[?0?????r(?/?vP<005),?E?5?B?????????????B???[???????0?????rmI???????????B???4???N?????????gVIP?] ???[?0?????r,?A?<?B???[???????????dmI[????]?????????????????R?P??????????,?????[?B???????d,???????????????P ?????r?????p?l???????N???????E??VIP?Y????????mI ???x??:????????;???????????B;?????N???T?d????;???????g;?5?g ?E???0????:R2855???x?7???V:A?????9??:10055509(2010)05065203 InfluenceofLongMuZhuangGuPowderonGastrointestinalFunctioninExperimentAnimalsHuangJuan,XuHui,LuoYanjuan,et alHubeiMaternalandChildrenHospital,Wuchang(430070) Abstract:[Objective]ToobservetheinfluenceofLongmuzhuanggupowder(LMZGP)ongastroentericmovementandguthormone inmice.[Methods]Toestablishthemouseofthedisorderofgastricdepletionandsmallintestimeadvancementbyusingmaxolon, atropine,neostigmineandadrenaline.Micewererandomlydividedintocontrolgroup,modelgroup,LMZGPlargedosegroup, LMZGPmiddledosegroup,LMZGPlowdosegroup.Gastricemptyingandintestinalprogradatingratesinmodelmiceweretested withblacknutritiousemisolidcataplasm.Theexpressionofvasointestinalpolypeptide(VIP)wastested byradioimmunoassay. [Results]MaxolonacceleratedgastricdepletionanddegradedthelevelofVIPingastroenterictissue.Atropineinhibitedgastric emptyandheightenedthelevelofVIPingastroenterictissue.Neostigmineacceleratedintestinepropagationanddegradedthelevel ofVIPingastroenterictissue.AdrenalineinhibitedintestinepropagationandheightenedthelevelofVIPingastroenterictissue. LMZGPadministratedorallyhadnosignificantinfluenceonthegastricdepletionandsmallintestineadvancementinnormalmice, butimprovedthegastroentericmovementinanimalmodelofgastroentericmovementdisorderswhichmanifestedasrelievinggas tricdepletiondisorderbyatropineandmaxolon,antagonizingsignificantlytheaccelerationorloweringofsmallintestineadvance mentinducedbyneostigmineandadrenaline.MiddleandlowdoseofLMZGPalsohadsomereverseeffect.LMZGPalsodosede pendentlyantagonizeddisordersofVIP.[Conclusion]LMZGPhasdualdirectionalregulatingeffectonintestinepropagationviaa mechanismofactionmainlyonVIPingastroenterictissue. Keywords:gastroentericmovement;Longmuzhuanggupowder;vasointestinalpolypeptide(VIP);gastroenterichormone;mouse ???????????6?V???????~??????(N63B63) FjIySB(N63B63) ?5?k?????????d?????K?0?W??,?E?T?A?????? ?5?k?????????d?????W???y??????,?????0?????v ?y?W?)??mV???A?W??mV?l?X??,???8??mI???? ???????B?p???6?V?E?;???d????,?T???r???????? ???J?5?k??????,??????;???5?k?l??mV?e??mV???J?) ?pmV???9?)??mV???????8?!?????[???????rmI?F?A?? ?????H?r??????????????????????????????????, ???s?????????????????M?????s?rmI 1?????<???? 11???????d?0?<???5?g,???/18~20g(4~6 ??),???????r,?E?????6?V?T???????????????? ?E??mI 65 ?????E?T???d??????2010??5?:?F34???F5?L :o:200AA011 undproect:temsupportedbHubeiProvincialci-techureauo:200AA011 2 ,?!???????????B????????????????????VI?????? 12???????B?y?????#VIP,?y?????B???E???6 ???????L?????p?A???NmI???????????B?v???!???R ???l?????L??;???)???v???????????l?[???????L ??;?k?????v?]???m???b???[???????L??;???????< ?v???e?s???b?????L??;???]???g?v???!???d?b?? ?????????[???????L??mI 13????OHAUSPT200S?j???s??,????????; Labofug400R?????v,????Heraeus????????mI 2???????? 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[2]??????.???????B???5?g???????????????v?b?????A?? [J].?E???E?T???N?T???B??,2004,10(6):4243. [3]?=?/.?E?????0?A????????[M].?6??:???R???L???= ?y,2006:438442. [4]?????3.?3???9?????????????9?)???v?T???????????? ?????3?N?3???????g????????????????[J].?E???E?T ???N?T???B??,2004,10(1):6366. [5]????.???I???u???????y???d?g?????????????????g?? ????[J].?????T????????,2006,29:100102. [6]???a??.???????:?B???d?g?????????????r?????v?b?? ??[J].?????E?T?B??,2005,32(12):13271329. [7]?g?!??.?????????????????????N?U?T?d???????9???A ??[]?E???T???d??????,6,35()3 (?b?k???L) 65 ?????E?T???d??????2010??5?:?F34???F5?L :P J.2002:18184. 20091109 4