不同浓度胰岛素对毛乳头细胞生物学作用探究

不同浓度胰岛素对毛乳头细胞生物学作用探究 不同浓度胰岛素对毛乳头细胞生物学作用 探究 [摘要]目的:了解不同浓度胰岛素对毛乳头细胞的生物学作用。:人头皮组织分离、培养、获得毛乳头细胞,根据胰岛素浓度分为1×10-8、1×10-7、1×10-6mol/L 3个组,设无胰岛素培养液为对照组。应用不同浓度胰岛素的2%新生牛血清培养液培养毛乳头细胞3天,分别用 MTT 法测定毛乳头细胞的增殖;AnexinV/PI 双染色法测定毛乳头细胞的凋亡情况;体外细胞划痕法测定其迁移能力。 结果:与对照组比较,1×10-8、1×10-7mol/L 胰岛素组毛乳头细胞增殖、抗凋亡及迁移能力显著增加( P 1.6统计学处理:采用SPSS 10.0统计软件包进行统计。数据以均数?差表示,组间比较采用单因素方差分析,两两比较采用LSD-t检验,P 1 [9]Krugluger W, Rohrbacher W, Laciak K. et al. Reorganization of hair follicles in human skin organ culture induced by cultured human follicle derived cells [J]. Exp Dermatol,2005,14(8):580-585. [10]Osada A and Kobayashi K. Appearance of hair follicle inducible mesenchymal cells in the rat embryo [J]. Dev Growth Differ, 2000,42(1):19-27. [11]Jahoda CA, Oliver RF, Reynolds AJ, et al. Trans-species hair growth induction by human hair follicle dermal papillae [J]. Exp Dermatol,2001,10(4): 229-237. [12]丁国斌,陈璧,韩军涛,等. 组织皮肤中人毛乳头细 胞体外培养条件的优化[J]. 中国临床康复, 2004, 8 (8) : 1450-1451. [13]Young TH, Lee CY, Chiu HC, et al. Self2assembly of dermal papilla cells into inductive spheroidalmicrotissues on poly( ethylene covinyl alcohol) membranes for hair follicle regeneration [J]. Biomaterials, 2008, 29 (26) : 3521-3530. [14]李宇,林常敏,蔡湘娜, 等. 微囊法体外重建人头皮毛 乳头诱导毛囊再生功能的评价[J].中国临床康复,2005,9(6): 46-48. 2 [15]Zhao J,Liu LQ,Wang YJ,et al. Treatment of alopecia by transplantation of hair follicle stem cells and dermal pap illa cells encapsulated in alginate gels [J]. Med Hypotheses,2008,70(5):1014-1016. [16]Suzuki R,Okada N,Miyamoto H,et al. Cyotomedical therapy for insulinopenic diabetes usingmicroencap sulated pancreatic beta cell lines[J].Life Sci,2002,71(15): 1717-1729. [17]De Fronzo RA.Ferran nini E.Insulin resistance:A multifaced syndrome responsible for NIDDM,obesity,hypertension,dyslipidemia,and atherosclerotic cardiovascular disease [J].Diabetes Care,199l,l4(3):173-194. [18]Nielsen C,Gormsen LC,Jessen N,et al. Growth hormone signaling in vivo in human muscle and adipose tissue: impact of insulin,substrate background, and growth hormone receptor blockade [J]. J Clin Endocrinol Metab,2008, 93(7):2842-2850. [19]Lataillade JJ,Cla YD,Bourin P,et al. Stromal cell-derived factor1 regulates primitive hematopoiesis by suppressing apoptosis and by promoting G0/G1 transition in CD34+ cells: evidence for an autocrine/paracrine 3 mechanism [J]. Blood,2002,99(4):1117-1129. [20]Peters R,Leyvraz S,Perey L,et al. Apoptotic regulation in primitive precursors [J]. Blood,1998,92(6)2041-2052. [收稿日期]2010-05-22[修回日期]2010-07-20 编辑/张惠娟 4