Product Name
Monoclonal Human
Anti- Citrullinated Fibrinogen Immunoglobulin, clone 1F11
CAT No.
MQR 2.101-100
Size
100μg
Edition: February 1, 2012
Page 1 of 1
Intended use
This product is for research use only. NOT for use in
diagnostic or therapeutic procedures.
A license from ModiQuest Research is required for use
outside the research field.
This product is tested for use in enzyme-linked immunosorbent
assay (ELISA), immunoblotting (IB), immunoprecipitation (IP)
or immunohistochemistry (IHC).
Reagent provided
The antibody has been lyophilized in a 10 mM ammonium
bicarbonate buffer.
Isotype
Human IgG1,
Specificity
Specificity has been tested in immunoblotting (figure 1) and
ELISA. Additional tests for cross reactivity have not yet been
performed.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources,
proper handling procedures should be used.
3. The product may be used in different techniques and
in combination with different sample types and
materials, therefore each individual laboratory should
validate the applied test system.
Preparation of the antibody
Recommended antibody concentration: 0.5 mg/ml
Recommended solvent; PBS or 100mM Tris-HCl, pH 7.0
Additional sodium azide ( up to 0.05%) is recommended
for long term storage.
For a 0.5 mg/ml antibody concentration, dissolve in
200 µl buffer.
NOTE: Be careful opening the vial since the antibody resides in
a vacuum.
Storage instructions
Store at 2-8
o
C.
For prolonged storage add sodium azide to 0.05%
Dilution guidelines
ELISA: 1:(200 x F) – (1000 x F).
Immunoblotting: 1:(200 x F) – (1000 x F).
Other applications: since applications vary, you should
determine the optimum working dilution of the product that is
appropriate for your specific need.
For the value of the multiplication factor F, see label on vial.
Unless the stability in the actual test system has been
established, it is recommended to dilute the product
immediately before use.
Relevance
Citrulline, while being an amino acid, is not built into proteins
during protein synthesis, as it is not coded for by DNA, yet
several proteins are known to contain citrulline. These citrulline
residues are generated by a family of enzymes called
peptidylarginine deiminases (PADs), which convert arginine
into citrulline in a process called citrullination or deimination.
Proteins that normally contain citrulline residues include myelin
basic protein (MBP), filaggrin, and several histone proteins,
while other proteins, like fibrin and vimentin can get citrullinated
during cell death and tissue inflammation.
Patients with rheumatoid arthritis often (at least 80% of them)
develop an immune response against proteins containing
citrulline. Although the origin of this immune response is not
known, detection of antibodies reactive with citrulline containing
proteins or peptides is now becoming an important help in the
diagnosis of rheumatoid arthritis.
Figure 1: Specificity of Anti-Citrullinated Fibrinogen Immunoglobulin, clone 1F11,
determined by Immunoblot analysis. Blot contains human fibrinogen (Sigma; cat no
F4883) in the left lane and rabbit PAD2 (sigma; cat no P4874) deiminated human
fibrinogen in the right lane. Incubated with antibody fraction (0.5 mg/ml) 1000X diluted
in PBS containing 0,05% tween-20 and 5% non fat dry milk.
References
1. Venrooij et al. Autoantibodies to citrullinated antigens in
(early) rheumatoid arthritis. Autoimmun Rev. 2006
Nov;6(1):37-41. Epub 2006 Apr 19.
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