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MQ13.102,单克隆鼠抗瓜氨酸纤维蛋白原免疫球蛋白(克隆20B2)

2013-05-04 1页 pdf 133KB 29阅读

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MQ13.102,单克隆鼠抗瓜氨酸纤维蛋白原免疫球蛋白(克隆20B2) Product Name Monoclonal mouse Anti- Citrullinated Fibrinogen Immunoglobulin, clone 20B2 CAT No. MQ 13.102-100 Size 100µg Page 1 of 1 Intended use This product is for research use only. NOT for use in diagnostic or therap...
MQ13.102,单克隆鼠抗瓜氨酸纤维蛋白原免疫球蛋白(克隆20B2)
Product Name Monoclonal mouse Anti- Citrullinated Fibrinogen Immunoglobulin, clone 20B2 CAT No. MQ 13.102-100 Size 100µg Page 1 of 1 Intended use This product is for research use only. NOT for use in diagnostic or therapeutic procedures. A license from ModiQuest Research is required for use outside the research field. This product is tested for use in enzyme-linked immunosorbent assay (ELISA), immunoblotting (IB), or Immunohistochemistry (IHC). Reagent provided The antibody has been lyophilized in a 10 mM ammonium bicarbonate buffer. Isotype Mouse IgG1 Immunogen Deiminated murine fibrinogen peptide. Specificity Specificity has been tested in ELISA (figure 1). Crossreacts with deiminated human fibrinogen. Additional tests for cross reactivity have not yet been performed. Purity Protein A purified. Precautions 1. For professional users. 2. As with any product derived from biological sources, proper handling procedures should be used. 3. The product may be used in different techniques and in combination with different sample types and materials, therefore each individual laboratory should validate the applied test system. Preparation of the antibody • Recommended antibody concentration: 0.5 mg/ml • Recommended solvent; PBS or 100mM Tris-HCl, pH 7.0 • Additional sodium azide ( up to 0.05%) is recommended for long term storage. • For a 0.5 mg/ml antibody concentration, dissolve in 200 µl buffer. NOTE: Be careful opening the vial since the antibody resides in a vacuum. Storage instructions Store at 2-8 o C. For prolonged storage add sodium azide to 0.05% Dilution guidelines ELISA: 1:(2000 x F) – 1:(4000 x F). IB: 1:(200 x F) – 1:(1000 x F). IHC: 1:(100 x F) – 1:(500 x F). Other applications: since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need. For the value of the multiplication factor F, see label on vial. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use. Relevance Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deiminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis. 0 0,2 0,4 0,6 0,8 1 1,2 1,4 Deiminated murine fibrinogen Murine fibrinogen Antigen A 4 5 0 Antibody fraction Blank Figure 1: Specificity of Anti- Deiminated Murine Fibrinogen Immunoglobulin, clone 20B2, determined by ELISA. Antibody fraction (0.5 mg/ml) 2000X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on both rabbit PAD2 (sigma; cat no P4874) deiminated as non deiminated murine fibrinogen. 世联博研(北京)科技有限公司独家授权代理荷兰modiquestresearch全系列产品 单位地址:北京市丰台区角门南华12-1507,邮编:100068 全国客服热线:010-67520703,QQ:1978161627,736597338 备有大量现货,详情咨询电话010-67529703
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