Product Name
Monoclonal mouse
Anti- Citrullinated Fibrinogen Immunoglobulin, clone 20B2
CAT No.
MQ 13.102-100
Size
100µg
Page 1 of 1
Intended use
This product is for research use only. NOT for use in
diagnostic or therapeutic procedures.
A license from ModiQuest Research is required for use
outside the research field.
This product is tested for use in enzyme-linked immunosorbent
assay (ELISA), immunoblotting (IB), or Immunohistochemistry
(IHC).
Reagent provided
The antibody has been lyophilized in a 10 mM ammonium
bicarbonate buffer.
Isotype
Mouse IgG1
Immunogen
Deiminated murine fibrinogen peptide.
Specificity
Specificity has been tested in ELISA (figure 1).
Crossreacts with deiminated human fibrinogen.
Additional tests for cross reactivity have not yet been
performed.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources,
proper handling procedures should be used.
3. The product may be used in different techniques and
in combination with different sample types and
materials, therefore each individual laboratory should
validate the applied test system.
Preparation of the antibody
• Recommended antibody concentration: 0.5 mg/ml
• Recommended solvent; PBS or 100mM Tris-HCl, pH 7.0
• Additional sodium azide ( up to 0.05%) is recommended
for long term storage.
• For a 0.5 mg/ml antibody concentration, dissolve in
200 µl buffer.
NOTE: Be careful opening the vial since the antibody resides in
a vacuum.
Storage instructions
Store at 2-8
o
C.
For prolonged storage add sodium azide to 0.05%
Dilution guidelines
ELISA: 1:(2000 x F) – 1:(4000 x F).
IB: 1:(200 x F) – 1:(1000 x F).
IHC: 1:(100 x F) – 1:(500 x F).
Other applications: since applications vary, you should
determine the optimum working dilution of the product that is
appropriate for your specific need.
For the value of the multiplication factor F, see label on vial.
Unless the stability in the actual test system has been
established, it is recommended to dilute the product
immediately before use.
Relevance
Fibrinogen is a protein produced by the liver which helps stop
bleeding by helping blood clots to form.
Fibrinogen gets deiminated (conversion from arginin to citrullin)
by Peptidyl Arginine Deiminase (PAD) in inflamed joints in
patients that develop rheumatoid arthritis.
Citrulline, while being an amino acid, is not built into proteins
during protein synthesis, as it is not coded for by DNA, yet
several proteins are known to contain citrulline. Proteins that
normally contain citrulline residues include myelin basic protein
(MBP), filaggrin, and several histone proteins, while other
proteins, like fibrin and vimentin can get deiminated during cell
death and tissue inflammation.
Patients with rheumatoid arthritis often (at least 80% of them)
develop an immune response against proteins containing
citrulline. Although the origin of this immune response is not
known, detection of antibodies reactive with citrulline containing
proteins or peptides is now becoming an important help in the
diagnosis of rheumatoid arthritis.
0
0,2
0,4
0,6
0,8
1
1,2
1,4
Deiminated murine fibrinogen Murine fibrinogen
Antigen
A
4
5
0 Antibody fraction
Blank
Figure 1: Specificity of Anti- Deiminated Murine Fibrinogen Immunoglobulin, clone
20B2, determined by ELISA. Antibody fraction (0.5 mg/ml) 2000X diluted in PBS
containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on both
rabbit PAD2 (sigma; cat no P4874) deiminated as non deiminated murine fibrinogen.
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