大豆异黄酮对氧化损伤血管内皮细胞的抗氧化作用
170
中国临床康复菪70巷舅7,期2006—03—20出版
饥i肥seJoⅡMf0,cfin诧越Re拗溉mlfo啊肘们^2D20D6yo正J0Ⅳo.JJ
·BASICRESEARCH·
AntioxidationofsoybeanisonaVoneinVascularendothelialceUswith
oxidatiVedamage★
LiuYing—hual,HuangGuo—weitChangHongILiuLitRenDa—IintXueChang—yong
1DepartmentofNut向on,G...
170
中国临床康复菪70巷舅7,期2006—03—20出版
饥i肥seJoⅡMf0,cfin诧越Re拗溉mlfo啊肘们^2D20D6yo正J0Ⅳo.JJ
·BASICRESEARCH·
AntioxidationofsoybeanisonaVoneinVascularendothelialceUswith
oxidatiVedamage★
LiuYing—hual,HuangGuo—weitChangHongILiuLitRenDa—IintXueChang—yong
1DepartmentofNut向on,GeneralHospitalofChinesePLA,Beiiing
100853,China;2LaboratoryofNutrition,InstituteofPublicHealth.Tianiin
MedicalUniversitv,Tianjin300070,China
uUYinq—hua★,Master,PhvsiciaIl,DepanmentofNutriti蚰,GeneralHo铲
pitalofChinesePLA,Be啪ng100853,China
R盹西ved:2005一05—30Ac唧ted:2005_07-05(05一ll_4—3608/G)
Abstract
BACKGRoUND:Sovbeanisonavonehasavarietvofbioactivitiesandits
antioxidationbecomesahotspotofresearchinrecentyears.Atpresent,the
research0fsoybeanis越lavoneplacesmoreemphasisonanimalexpeTiment
andclinicalobsenration.butlacksresearchDncellular1evelofhumanbodv.
oBJECrrIVE:To0bservetheillfluenceofsoybeaIlisoflavoneinvascul8r
endothe王iaIcellswi£hoxidativedamage.
DESlGN:ContIDlledtrialandobservation.
SETrDqG:CentralLaboratow,Institute0fPublicHealth,Tian讧nMedical
University.
MATERk札S:TheexperimentwascompletedintheCentralLabomtory,
InstituteofPublicHealth,TianjinMedica】UniversityfIDmJaIluarytoJuly
2002.Theelpe矗mentalmaterialsincludedvascularendothelialce】lstrain
inhumanumbilicatvein,Iowdensitylipoprotein,soybeanisoftavoneandvj.
t∞inE.efc.
M凰THoDS:Thevascularendothelialcellswerecultured流秽i£ro.Theex—
perimemwasdividedinto6gmups:blankcontmlgroup,o)【idativedamage
controlgmup(malondialdehydecontentwasl斗mol/L),oxidativedaIIlage+
vitaminEcontrolgmuP(vit踟inEwas50¨mol/L)andoxidatived黝age+
soybeallisofla蚰ne】O,50,Joo“m01/Lcont叫group.neendothebdcells,
whichwerejojnedwithvit砌inEandsoybeani50navoneofdifferenccon—
centmtionsinadVancetobeincubatedfor24hours,wereafkctedbyoxi—
dizedlowdensi‘ylipoproteinsandthenculturedcontilluallyfor24hours.
AlltheindexesofantioxidationweIedeteHninedinbotllextI矗一cellaJldin.
tra—cell.
hIA】烈oUTCoM[Eh僵EASURES:Malondialdehydecontent,activityof
superoxidedismutaseanddutathioneperoxidase,thereleaseeonditionof
Iactatedehydrogenaseandpmductivequant.【yofnitrogenmonoxideinside
andoutsidetheendothelialcellsofeachgroup.
IUeSULTS:(量)Comparisonofmalondialdehydecontent,theactivityofsupe卜
oxidedismutaseandglutathion8per似idaseinendothelialcells0feach
group:Themalondialdehydecontentwashighersignificantlyinoxidative
damagecontmlgroupthaninblankcontrolgroup(P<0.01),buttheactivity
ofsuperoxidedismutaseandglutathjon。peroxidasewas10wer蝻gnj行cantly
inoxidatived枷agecontrolgroupthatlinbIankcontrol舯up(P
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