细环病毒-从实验室到临床

细环病毒：从实验室到临床Torquetenovirus:thehumanviromefrombenchtobedside文献汇报D.Focosietal.ClinicalMicrobiologyandInfection22(2016)589-593TTV:TransfusiontransmittedvirusorTorquetenovirusFromtorquesandtenuis,Latinfor‘necklace’and‘thin,’respectively输血后肝炎（肝中病毒载量是血中的10-100倍，并且与血清ALT升高相关）【现已否定该结论】Humanviromeatleast29majorspecies细环病毒：首个被发现的单链环状DNA病毒TTVprevalence>95%inmostcasesindependentofage,socioeconomicstandingandhealthconditions来源：胎盘、呼吸道、输血分布：T淋巴细胞现有临床应用：TTVasaMarkerofAnthropicPollutionTTVasaMarkerofImmuneStatusOrphanvirus~孤儿病毒（非病原性病毒）水源是否被污染：现有方法为检测细菌，但是很容易出现假阳性某些情况下即使细菌检测为阴性也不能排除被污染检测病毒更准确TTV分布广泛：医院空气、物件面均可存在TTVasaMarkerofAnthropicPollutionTTVasaMarkerofImmuneStatus免疫功能完整性：theabilitytomountaprotectiveimmuneresponseagainstanantigenicstimulation.现有检测：抗原特异性，抗原非特异性；检测繁琐，检测方法缺乏化TTV与CD8+57+T淋巴细胞比例成负相关对抗病毒药不敏感4TTVasaMarkerofImmuneStatusexcessive(toomanyopportunisticinfectionsandcancers)inadequate(toomanyepisodesofcellularacuterejection)Maintenanceimmunosuppression5WhyIsTTVNotYetPartofaDiagnosticToolKit?现有研究不足无法培养无抗体检测试剂无动物模型PCR方法缺乏标准化（引物）特异性引物非特异性引物6TakehomemessagesTTVisatypeofOrphanvirus,prevalence>95%inmostcasesTTVreplicatesmainlyinTlymphocytesInsensitivetocurrentantiviraldrugsTTVasaMarkerofImmuneStatusPredictingopportunisticinfectionsPredictinggraftrejection7