H9N2亚型禽流感病毒NS1基因的原核与真核表达

! !" " ! ! #$ # %$#$ $ #$ % !"#$%&'(() ! &'()* " &'()*+,'-.')/0123/4567*892)38/ : ! .+/;<=8;>?; " @',;!" .';#$ A=/;%$#$ =S>% ¶·¸¹ºxy $*# ^_6 “”O»”`i " 2XY # ! :Z[ # ! 5\L # ! % ! ]O^ # ! _ ! ` # ! abc # ! def # ! # +,-.(/0) 123)4 # 5+ LM B#%#$$ $ % 5+LM”b:2EF¸¦z{ # 5+ LM B#%#$$ " ! 9 ! : " ! % VW & ëÓ TS.% ƒœ¶Àhüý ! 4Y@ " 5=# ’“WšÎwŒÎ”•4m # d'8†z0Teå w %S! CDJÉʔ• # ƒÉ3dHI .<# åæW…]‰'s™ÒWð‹Ão # ½‰`t¶Àh§"¦§¶w2ý h˶ >VY<4 uvd UWij‡©@»ª6 '% bc & o KOPIJK bcG¯ TS.% ƒœ 4Y@ W 5=# ’“ # d' t^›œõšÎ”•4m F >OP!%+ ! N " JëÓ 5=# ’“WšÎ;³è– # â`0Teå EV%# ! L>! " ´ # o¶ó’ô h79ð“ ! YIO_ " YZ”• $ / # d 5=# ’“›œõŒÎ”•4m F >_6IPJ# J # ëÓ 5=# ’“ŒÎ;³è– # d'ª/âË %S! CD # C"0 ´¾èhËCD # <0Te唕|2w %S! CD”•|2ÉÊ j23/2)*PH,'//8* U 89 ' % –— & š…ëÓ} 5=# ’“WšÎ”•4m F >OP!%+P.<# wŒÎ”•4m F >_6IPJ#P.<# ' j23/2)*PH,'//8* U – —”g # 0Teåw %S! CDW”•|2·]Œõµ¶[€' ! 0TeåW”•|2zÑ CC\( ñïî©[€' # %S! CD”•|2zÑ DD\( ñï€' "'% –» & 5=# ’“z0Teåw %S! CDJôõš…”• # (ôW .<# åæ)¸UVW…š£[ ' ! ;<= " ! TS.% ƒœ¶Àhüý $ 5=# ’“ $ 0Teå $ %S! CD $ šÎ”• $ ŒÎ”• ! >?7@A " ! <"DD;! ! BCDEF " ! 4 ! BGHA " ! #QB#PS!"B ! %$#$ " #$P$$%BP$Q 7),8+) . ,*'%+"#&$%+) . ,*'%&9 2 )&//',",(>S?% +1'+"'"(3$&"<+1')$/$2# 5 &"& j4._&8* U P : ( # # VY7T'* U P : +* # # VYT2P,8* # # % # ZT4._<+*P?'* U # # VY7J0+' # # j>.X8+'P0(8 # # V>YM2* U P/'* U # ! ##&''( ) (& * +(,(-./0- 1 2(3.4./( # 5&-,67(8,"9:;/.<(-8., 1 # F0/ ) './ ) # =600/>.B#%#$$ # #6./0 $ %@< * 0/ ) C.&,(46/&'& )1 @.D.,(3#&D O 0/ 1 # F0/ ) './ ) # =600/>.B#%#$$ # #6./0 " !"#$%&'$ )% AH [ 2=/892 & O023/(? : 1+3/'='*3/)(=//02 F )'\+) : '/8=2] F )2338'*92=/') F >OP!%+P.<#+*? 2(\+) : '/8=2] F )2338'*92=/') F >_6IPJ#P.<#-')/022] F )2338'*'-/025=# U 2*2'-TS.%+98+*8*-,(2*a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a2?18/0j23/2)*PH,'//8* U ; % K23(,/ & K23(,/330'12?/0+//02 F )'\+) : '/8=2] F )2338'* 92=/')+*?2(\+) : '/8=2] F )2338'*92=/')'-5=# U 2*212)23(==233-(,, : ='*3/)(=/2? # /023 F 2=8+,H+*?'-.<# F )'/28*2] F )2332?8*EP4&'.+*?%S!=2,,312)28?2*/8-82?H : j23/2)*PH,'//8* U ; % J'*=,(38'* & 5=# U 2*21+3 " + ¾¿À# , ! %$#$P$!P%C + ’ÁÂV , ! 5+6(/«þÂV ! %$$D^ $#P_%$P% " + ÃÄÅp , ! G¬­ ! #SQC` "# Û # 5+®æÎ # ZÝÞ # ÐÑ # çÑ:Zß # ÒÓÔÕP¶ ËüwR3zª¿:)HI ' >PG+8, ) 1 [ 8* U: (%$$C " #%Q;='G 3(==233-(,, : 2] F )2332?8*EP4&'.+*?%S!=2,,3 # j23/2)*PH,'//8* U /23/30'12?/0+//02.<# F )'/28*30+?92) : U ''?+*/8 U 2*8=8/ : ; () * +,%-# ) TS.%+98+*8*-,(2*a+98)(3 $ 5=# U 2*2 $ EP4&'. $ %S!=2,, $ F )'\+) : '/8=2] F )2338'* $ 2(=+) : '/8= 2] F )2338'* !! ¶Àh ! 498+*8*-,(2*a+ # 4Y " WVaìíh¶ 7WiŽ;W§ü ' ¶Àhüý’“³r " €« ) ( ¯Æ ( 8y™W K.4 g™ëš # 'Jg™ " f 5= ’“ # ¸ % -ÏÐ/ # ìÐ .<# w .<%% xå æè # 'J .<# ƒs–ëåæ # .<% ƒ–ëåæ ' 4 œÀhüý 5= ’“>?Wá[7¶|0 # ¼ ½8ƒçӒ“ 4 w E # «Ä4Wá[}ƒ B%;!d fg # k3çӒ“Âäá[° # • S$d ½+ + # , ' ¶Àhüý 5=# ’“Îß E kçÓ ’“ # zÉ`J°ÁkÙ ' 2q # .<# åæzÀh üýhËW¢#°0¨”• # k}ïîßhËCD ä # lÒÓçèzCDÎä # ]YZ±m|:… .<# åæW…m # i¶Àh#£§"¦§W¶Â~]| :ð…m # “K .<# åæ¼Ãƒ3x`†uv  # osô82ýhËw#£§"¦§W¶Â # / .¼Ãƒƒv¢#hËWª6 + % , ' .<# åæ}zü ýç!±mCD´±]˜§š # ó‡± .<# ¼]z '²öüýhËWCD¥FJ³È•3-;Ӂ ‘ # <ÀhüýWý[V•sS;ÓWÃo ' `ß 5=# ’“&%Wµ< # 'z`†uvJ¸•\oW `oab ' ƒK # cHIÔ5+6 TS.% ƒœ¶À hüý8rÆ ! j. Æ " J›œõ} 5=# ’“ # d' 8†z0Teåw %S! CDJÉʔ• # ½#ƒÉ 3dHI .<# åæW…]‰'s™ÒWð‹Ão ‡©<= # .ƒ¶Àh§"¦§¶w2ýh˶W `† >VY<4 uvd UWij‡©2è’e ' # ! Z[sbc #;# ! I ! J #;#;# ! pq`rs ! TS.% ƒœ¶Àhüý j. ! 4 * J08=\2* * <0++*]8 * $# * %$$" " Æ # r+,-.(/ 0)P¶;0§ü$QwP|}:2®~=>?k l $ #$ Àe ! " h{ÒCD‰ %S! CD # ·r+,-.(/ 0)P¶;0§ü$QwP|}:2®~=>?k l ' #;#;! ! $%&' ! O)8a',K2+ U 2*/ ( V8 F '-2=/+G8P *2 OM %$$$ ïèm # ·S& Y*98/)' U 2* z{ $ F MLP #"O<8G F ,2 ›œ4md U ( 4M@ â㗠( OC L.4 ¡º—ç # ·S&ö:2 ! 0¡ " EF¸¦z { $ šÎ”•4m F >OP!%+ ! N "( ŒÎ”•4m F >_6IPJ# ( TS.% ƒœ©[…nÍ # ·r+,-. (/0)P¶;0§ü$QwP|}:2®~=> ?kl $ àXnÍ ( LM>M gh’ # ·S& _YEJA z{ $ TKIP ’…´ Y U _ —–§2 # S&°«:2 EFz{ $ 'jd ·ƒ <8 U G+ 8µ*@|89O ¶ ' #;% ! xy6ˆ¼ d TS.% ƒœ¶Àhüý j. ! 4 * J08=\2* * <0++*]8 * $# * %$$" " Æ]?'(´ºxß #$ Àe 7$"Q%DQ ( LRSSBC"C " W’“> ? # o I)8G2)D;$ :@3 .<#6# * .<#K# w .<#6% * .<#K%% < 5=# ’“µ¶[¢2 # oßG ¯ 5=# ’“ ' ¢2>?C‚ ' .<#6# ) DhP44__4OJJ4O__4OOJJ44J4 JO_O_OJP!h # .<#K# ) DhP_JJOJ_4_JO4JOOO__4___ 4_O_P!h $ .<#6% ) DhPJ44_44OOJ44O__4OOJJ44 J4JOP!h # .<#K% ) DhPOO__4OJJOJO4JOOO__4_ _ * 4_4_OP!h ' 2q # z¢2 .<#6# ( .<#K# ( .<#6% ( .<#K% W Dh •8†¢! C0DT ( ( U6&V ( ( E4&K ( w C0DT ( —1Ó< ! ‚ß:Á8 "' ¢2·r+£: 2EF¸¦z{§š # é#G¯g™žÁƒ QDCH F ' #;C ! xy{ K.4 6|} KOPIJK ‡ˆ É Y*98/)' U 2* z{W O)8a', \ÃHgI # ຠԴl!žmJ‡ˆ TS.% ƒœ¶Àhüý’“ 7. +,-.(/0))Ì ! &'()* " ! !" " ³ K.4 # ;6 MEY z{ 62)G2*/+3 âãd U HgI§š =L.4 ' IJK `mŠƒ D$ $ V ) Fú =L.4C $ V # + ( ‚c¢2 ! #$ F G', * V " ² # $ V # De IJKH(--2)#$ $ V # $0 M L.4 秗 ! D7 * $ V " $;D $ V # Œ#åþO¹Þ D$ $ V ' G¯`ûLM< ¾ƒ ) SDc éþ[ DG8* $ SCcD$3 # DBcCD3 ! ¢ 2 .<#6# * .<#K# "* Q% c CD3 ! ¢ 2 .<#6% * .<#K% "# B%c#G8* # !D -LM $ B%c ‹JK #$ G8* $ Cc kl ' ˆ IJK |2 D $ V # o #$ U * V îï ð´µPQüý–— ' #;D ! 5=# ^_6 8qrPQ ˆ 5=# ’“W IJK G¯|2 # É L.4 €àO `V¾d UHgIV¾VWg™ # <- F MLP#"O <8G F ,2 ›œ4md UyoHgI # d IJK O` V¾|2s F MLP#"O<8G F ,2 4m¡º # 8)· ´ # ß #Qc ¨©¹bJ¡º¥) ' ¡º|2â` LTD ! 0Teåh{ÒCD # !Bc ¨©ªJgh #% % #"0 # §åR:žUVi„gð‹;¦/÷ˆ 摫-åR # ºxõÂpjÃÄÅ ! #$$ $ U * GV " W VE mmgh’Jgh¥) # ‡ˆè– ' ¤åR IJK ! mŠwF> #;C "( è–d—1w>`t ·ƒ©[Wè– # 8†ßàƒ IMLP.<# : w IMLP .<#a ' #;Q ! 5=# ^_“”»”`i½g6¾¿ #;Q;# ! uvBCwx-yz ! o¦i[ä1— C0DT ( ( U6&V ( /Ù`è– IMLP.<# : wš Δ•4m F >OP!%+ ! N "# V¾VW’“w”•4 m # ëӚΔ•4m F >OP!%+P.<# # ÉÊåR IJK ( è–d—1w>`t # ‡ˆ`têW©[ è–jo ' #;Q;% ! {vBCwx-yz ! o¦i[ä1— E4&K ( ( C0DT ( /Ù`è– IMLP.<#a wŒÎ ”•4m F >_6IPJ# # V¾VW’“w”•4m # ë ӌΪ/”•4m F >_6IPJ#P.<# # ÉÊåR IJK ( è–d—1w>`t # ‡ˆ`têW©[ è–jo ' #;B ! ¡¢ 5=# ^_À'ÁÂÃ>6`i ÷ˆâ` F >OP!%+P.<# ;³”•è–½‰â ` F >OP!%+ ! N " Oè–W EV%# ! L>! " h{ÒCD «›œ # ºxß !GV ÂpjÃÄÅ ! #$$ $ U * GV " W VE mmgh’J # !Bc lmgh¥) # öÀÉm ½8¾ #d ºx¨G0ghÞ AL Q$$ • $;Q % $;" / # v! YIO_ ÞùúÁƒ $;CGG', * V # %Qc Y Z”• D0 ' #;" ! ¡¢ 5=# ^_À %S! 12>6`i d %S! CDghzÂm½8¾ #$d àXnÍ # ~Âà ( ÆÄÅW°ð LM>M gh’J # âËa3 DºxCDÞ Q ùghú # ºx¨½! % DCDW ~§Á•õ S$d % SDd ƒŠ ' É- V8 F '-2=/+G8P *2 OM %$$$ ïèmHgIo;³è– F >_6IPJ#P .<# âË %S! CD # /@O4m F >_6IPJ# âË <-³wgâË %S! CD<-³ ' âËCDß !B c ( ¿wÀÁ ( m½8¾ Dd JA % ghªJgh # ô 4¸ #%0 # oŽ­®¯üýâ˄…‰ª/”• ./ # C"0 ´¾èCD ' #;S ! 5=# ^_`iŸb6  j23/P 2)*PH,'//8* U 7s ¾è0TeåYZ”•|2 # ¤ñ@´ÉÊ #%$ U * V 89 # 3Á8´µo=01 Ëˑ # 23Á8oßâ¹íÉÊ j23/2)*PH,'//8* U 89 ' ¾èâË´ C"0 W %S! CD # ñ@´ÉÊ j23/2)*PH,'//8* U 89 ' % ! –—s89 %;# ! 5=# ^_6 KOPIJK ‡ˆ ½üý K.4  â ã | 2 ƒ F ú # 8 † o .<#6# * .<#K# w .<#6% * .<#K% % < ¢ 2 < TS.% ƒœ¶Àhüý j. 8rÆ 5=# ’“ÉÊ IJK G¯ # |2z #$ U * V îïð´µJ # ·¼~ # € QD$H F fgWµ¶[€' ! _ # "# žÁsé#– —ð? ' %;% ! 5=# ^_6qr d % <¢2G¯W 5=# ’“ IJK |2V¾´ s F MLP#"O<8G F ,2 4m¡º # â` LTD ! h{Ò CD # ÷ˆæ‘åR‡ˆè–´ÉÊd—1`t # · ôõÑ %B$$ w QD$H F W % €' ! _ % "# sé#– —ðA # ”g 5=# ’“5š…›œõ4mJ ' %;! ! 5=# ^_“”»”¡¢­Ä6PQ < 5=# ’“šÎ‰ŒÎ;³è–WåR # ÉÊ IJK `t·¼~ # € QD$H F fgWµ¶[€' ' d 5=# ’“šÎ;³è– F >OP!%+P.<# o C0DT ( w U6&V ( d—1ôõ DS$$H F W F >OP!%+ ! N " 4m€'w QD$H F W 5=# ’“€' ! _ ! "$ d 5=# ’“ŒÎ;³è– F >_6IPJ#P.<# o E4&K ( w C0DT ( d—1ôõ D$$$H F W F >_6IPJ# €'w QD$H F W 5=# ’“€' ! _ C "' ¤d^>89 # ÎE§šW 5=# ’“s _2*E+*\ +zâW@ä' j TS.% ¶Àhüý 5=# ’“Γvá[ƒ 8. ! #$ # G¬­ # ç ) TS.% ƒœ¶Àhüý 5=# ’“WšÎsŒÎ”• SQd % S"d # p’vá[ƒ "Bd % SBd $ sJ@ º»w@q8rÆ{| # §šW 5=# ’“z¼’• ¸ #! -p’vWÔÕ # Šlz<^þ # }¸p’v ½!îÚ # A§J@0ø TS.% ¶Àhüý8rÆ W‘µò ' ½+–—”g # VW’“5ꁛœ õšÎwŒÎ”•4mJ ' _ # ! 5=# ’“ KOPIJK G¯|2WPQ–— M;L.4M+)\2)LV%$$$ $ #;5=#IJK G¯|2 ! .<#6# * .<#K# "$ %;5=#IJK G¯|2 ! .<#6% * .<#K% " 68 U ;# ! 4 U +)'322,2=/)' F 0')2383'-KOPIJK F )'?(=/3'-5=# U 2*2 M;L.4M+)\2)LV%$$$ $ #;5=# U 2*2IJK F )'?(=/ ! .<#6# * .<#K# "$ %;5=# U 2*2IJK F )'?(=/ ! .<#6% * .<#K% " _ % ! 5=# ’“;³è–W—1`t M;L.4M+)\2)LV%$$$ $ #;IMLP.<# : W C0DT ( w U6&V ( d—1|2 $ %;IMLP.<#a W E4&K ( w C0DT ( d—1|2 68 U ;% ! Y?2*/8-8=+/8'*'-5=# U 2*2)2='GH8*+*/ F ,+3G8?H : )23/)8=/8'*2*?'*(=,2+32 M;L.4M+)\2)LV%$$$ $ #;IMLP.<# : ?8 U 23/2?18/0C0DT ( +*?U6&V ( $ %;IMLP.<#a?8 U 23/2?18/0E4&K ( +*?C0DT ( _ ! ! šÎ;³è– F >OP!%+P.<# W—1`t M#;L.4M+)\2)LV#D$$$ $ M%;L.4M+)\2)LV%$$$ $ #;C0DT ( «—1 F >OP!%+P.<# $ %;C0DT ( w U6&V ( d—1 F >OP!%+P.<# 68 U ;! ! K23/)8=/8'*+*+, : 383'- F )'\+) : '/8=)2='GH8*+*/ F ,+3G8? ! F >OP!%+P.<# " 18/0C0DT ( +*?U6&V ( M#;L.4M+)\2)LV#D$$$ $ M%;L.4M+)\2)LV%$$$ $ #;K2='GH8*+*/ F ,+3G8??8 U 23/2?18/0C0DT ( $ %;K2='GH8*+*/ F ,+3G8??8 U 23/2?18/0C0DT ( +*?U6&V ( _ C ! ŒÎ;³è– F >_6IPJ#P.<# W—1`t M#;L.4M+)\2)LV#D$$$ $ M%;L.4M+)\2)LV%$$$ $ #;E4&K ( w C0DT ( d—1 F >_6IPJ#P.<# 68 U ;C ! K23/)8=/8'*+*+, : 383'-)2='GH8*+*/2(\+) : '/8= 2] F )2338'* F ,+3G8? ! F >_6IPJ#P.<# " ?8 U 23/2? 18/0E4&K ( +*?C0DT ( M#;L.4M+)\2)LV#D$$$ $ M%;L.4M+)\2)LV%$$$ $ #;K2='GH8*+*/ F ,+3G8??8 U 23/2?18/0E4&K ( +*?C0DT ( %;C ! 5=# ^_“”`iŸb6  j23/2)*PH,'//8* U 7s PQ–—­± # ôõ}é#Wåæ €' # '89è¨Ñƒ CC\( # iO4m }gïî Kµ¶[€' ! _ D "# 8d”gð CC\( å攕€ 'ƒ F >OP!%+P.<# W”•|2 ' j23/2)*PH,'//8* U 89–—­± # šÎ”•W .<# M§åæzÑ CC \( ñïî©[€' ! _ Q "# ”gS”•Wåæ)¸ ¦§)£[ # ƒtS”•Wåæƒ .<# åæ ' %;D ! ÅÆ»”¡¢­Ä %S! 126ÇÈÉÊËÌ ÍÎ ëÓW F >_6IPJ#P.<# ;³è–zâË %S! CD %C0 ´ # Ž­®¯‚üýŽî # O4m F >_P 6IPJ# w;³è– F >_6IPJ#P.<# â˳CD· ¼~”‘Ž # O4m<-³”‘Ž¾¤ßÖ- CDJ # i;³è–d>³Ž\g­èJßÎä # 9/ +,-.(/0))Ì ! &'()* " ! !" " sé#ðA # ”g .<# zmqš…(ô}”• ' %;Q ! 5=# ^_»”`iŸb6 j23/2)*PH,'//8* U 7s âË;³è– F >_6IPJ#P.<# WCD }z Ñ DD\( ñs TS.% ƒœ©[…n͸µ¶[ ` # ïîVW€' # iN[<-zKñÕ34€' ! _ B "# ”g;³è–z %S! CDJš…”• ' _ D ! 5=# ’“z EV%# ! L>! " ™ÒåJW”• M; åæè89¨„ $ #; F >OP!%+ ! N " O4må $ % % "; YZ D0 ´W F >OP!%+P.<# ;³å 68 U ;D ! >] F )2338'*'-5=#8*EP4&'.EV%# ! L>! " M;I)'/28*G',2=(,+)128 U 0/M+)\2) $ #;.2 U +/892='*/)','- F >OP!%+ ! N " 8*?(=2?H : YIO_ $ %` "; F >OP!%+P.<#8*?(=2?H : YIO_ _ Q ! .<# ”•åæW j23/2)*PH,'//8* U `t #; ;³è– F >OP!%+P.<# s\… Y U _ W` $ %; O4mè– 68 U ;Q ! Y?2*/8-8=+/8'*'-/023 F 2=8-8=8/ : '-2] F )2332?.<# F )'?(=/3H : j23/2)*PH,'//8* U #;>] F )2338'*'--(38'* F )'/28* $ %;.2 U 8/892='*/)', _ B !F >_6IPJ#P.<# âË %S! CDW j23/2)*PH,'//8* U Œ #; âË F >_6IPJ#P.<# è–WCD $ %; gâËWCD 68 U ;B !F >_6IPJ#P.<#/)+*3-2=/2?%S!=2,,3 8*j23/2)*PH,'//8* U /23/ #;J2,,3/)+*3-2=/2?18/0 F >_6IPJ#P.<# $ %;J2,,3(*/)+*3-2=/2? ! ! m ! » ! # " rßÀhüý¼]W¿33-¼½zò- %äy‚Y+ÎÂ0¨hËWüšm # S½ö÷ƒ . Îki´W0À§ /# ¢V}‚Y²@W°Á;1 # “K$-¶ÀhWŽ:s ªšƒHIW;<ƒ 3 + ! , '  ·WÀh#£§" # |'¼½Ÿi¦§1 2ïîÌP Ñwîï # k~]Á-hËwwý # i TY ( >VY<4 çS[Œ–ëåæ…mWbcÕcô 8&'hË12s#£§"¦§12 # óœ¶Àh W¬¯v}ÂÁ ' .<, åærß'&%Wµ< # z§"¦§w2ýh˶W`†uvb͸•\] W`oab ' E8)=0PM+)=08* ç + C , w Aa+\8 ç + D , 8 †`o >VY<4 /GŒ .<, …m # ]š…°ô8À hüýhË0w#£§"¦§0 ' O(G F 2 : ç + Q , ¢ o;³ .<, åæwÎE§šW .<, \ ! .<, %S` C# w .<, !Q` C" " Ś # ÓÔ}ô82ýhË´Âw #£§"¦§´ÂWbc ' GÃÄç + B , úo¶Àh üý;³ .<# åæ̍…š # ÓÔ}Œ 4Y@ .<# W4ºÁv >VY<4 bc # ­±}UVW`oa b # kVaýÕÿ}`W­<¶ÀhüýW .<# … m >VY<4 Œd U ' ! % " cHIno KOPIJK bc›œï # Æ TS.% ƒœ¶Àhüý j. 8rÆ 5=# ’“ # ©[ ›œ>–—”g # ›œW TS.% ƒœ¶Àhüý j. 8rÆ 5=# ’“sé#–—ðA ' 5=# ’“ >?89”g # j. 8rÆ 5=# ’“>?s _2*P E+*\ zâW@ä'j TS.% ¶Àhüý 5=# ’“ WΓvá[ƒ SQd % S"d # p’vá[ƒ "Bd % SBd $ sJ@º»w@q8rÆð{ # §šW 5=# ’“z¼’•¸ #! -p’vWÔÕ # Šlz <^þ # }¸p’v½!îÚ # óA§J@0ø TS.% ¶Àhüý8rÆW‘µò + "PS , ' ! ! " .<# åæzÀhüýÇi‰sCDåæW ð‹ÃoJŽ/•;ÓÃo ' .<# åæÅt}üý z™ÒCDäW'²Ão # <ïgÀhüýWyü ±@¸;Ӑ‘ + #$ , ' .<# åæ)¸… ( œBÆÅ WÃo # ]DžBÆÅW… 4 œÀhüýhËà :/ ! #$ # G¬­ # ç ) TS.% ƒœ¶Àhüý 5=# ’“WšÎsŒÎ”• o + ## , ' 6+,=m* ç + #% , HIŽî # .<# åæ_6IPJ# 4mW89 ¨ƒ !!\( # 5=# ’“W89¨Ñƒ %!\( "# sé# –—ðA # ”gâËCDJ .<# åæôõš…” • # l)¸UVW¦§)£[ ' / # úoM§”• b€z0TeåJ”• 5=# ’“ # – —­± # 5=# ’“z0TeåJôõš…”• $ j23/2)*PH,'//8* U ô õ Ñ CC\( W µ ¶ [ € ' ! F >OP!%+ ! N " 4mW89¨ƒ %#\( # 5=# ’“W 89¨Ñƒ %!\( "# f=”•Wåæ)¸UVW¦ §)£[ ' 5=# ’“z %S! CDw0TeåJW š…”• # ƒÉ3dHI .<# åæW…]‰'s™ ÒWð‹Ão‡©}ª6 # /.ƒij¶Àh§ "¦§¶w2ýh˶W >VY<4 uvd U‡© }2è’e ' ! jkBC " + # , ! ŸÈG ; 89üý) + M , ; ,Á ) )Éï*³ # #SS$ ) !%#P!%%; T'(b L;M',2=(,+)98)',' U: + M , ;E28 [ 8* U ) X(2 : (+*I)233 # #SS$ ) !%#P!%%; ! 8*J08*232 " + % , ! ¼ÊË # è̲ # ¼ÍÕ ; ¶Àhüýs–ëåæWHIɏ + & , ; ±.PQR3 # %$$Q # %B ! S ") #DP#B; Z0+'6& # V8&J # Z0+'b&;O02)232+)=0 F )' U )233'*+98+*8*P -,(2*a+98)(3 F '*3/)(=/()2 F )'/28* + & , ;&8,8*4*8G+,<=82*=2+*? @2/2)8*+) : M2?8=8*2 # %$$Q # %B ! S ") #DP#B; ! 8*J08*232 " + ! , ! èÎ$ # ÏGÊ ; þ߶ÀhŽ:s ªWÐÑVÒsHIÉ  + & , ; J@12kÓ # %$$" # ##! ! B ") S$PSD; V8^. # V(L<;T83/')8=+,)29821+*?)232+)=0 F )' U )233'-/02 '==())8* U +*?3 F )2+?8* U '-+98+*8*-,(2*a+98)(3 + & , ;J08*+4*8P G+,T2+,/0 # %$$" # ##! ! B ") S$PSD; ! 8*J08*232 " + C , ! E8)=0PM+)=08*Y # K'1+*4 # I8=\& # 2/+,;>] F )2338'*'-/02*'*P 3/)(=/()+, F )'/28*.<#'-2 g (8*28*-,(2*a+498)(3 ) ?2/2=/8'*'- +*/8P.<#+*/8H'? : 8* F '3/8*-2=/8'*2 g (8*232)+ + & , ;@8)', M2/0'?3 # #SSB # QD ! % ") %DDP%Q!; + D , ! Aa+\8T # <(38GO # <( U 8/+< # 2/+,;L2/2=/8'*'-+*/8H'?823/'/02 *'*3/)(=/()+, F )'/28* ! .<# " '-8*-,(2*a+498)(3+,,'13?83P /8*=/8'*H2/122*9+==8*+/2?+*?8*-2=/2?0')323 + & , ;@2/M8=)'P H8', # %$$# # "% ! % ") ###P##S; + Q , ! O(G F 2 : OM # 4,9+)2aK # <1+ : *2L> # 2/+,;L8+ U *'3/8=+ FF )'+=0 -')?8--2)2*/8+/8* U 8*-2=/2?-)'G9+==8*+/2? F '(,/) : '*/02H+383'- +*/8H'?823/'.<# # /02*'*3/)(=/()+, F )'/28*'-8*-,(2*a+498)(3 + & , ;&'()*+,'-J,8*8=+,M8=)'H8',' U: # %$$D # C! ! % ") QBQPQ"!; + B , ! GÃÄ # ýÔ¯ # ÕÉ° # ç ; ¶Àhüý .<# …m4ºÁv >VY<4 ŒbcWÓÔ + & , ; J@R3() # %$$S # !S ! B ") Q#%P Q#Q; j+* U ZI # <0+* U XZ # <(*&T # 2/+,;>3/+H,830G2*/'-+*8*?8P )2=/PH,'=\8* U >VY<4-')?2/2=/8'*'-+*/8H'?823+ U +8*3/.<# F )'/28*'-+98+*8*-,(2*a+98)(3 + & , ;J08*232@2/2)8*+) : <=8P 2*=2 # %$$S # !S ! B ") Q#%PQ#Q; ! 8*J08*232 " + " , ! J+)',8*2E+*2/P.'+=0 # 4,2]+*?2)I+*308*;_2*2/8=+*+, : 383'- *'*3/)(=/()+, U 2*23 ! .<#+*?.<% " '-TS.%+*?TD.#98)(P 323)2=2*/, : 83',+/2?8*Y3)+2, + & , ;@8)(3_2*23 # %$$B # !C ) #DBP #Q"; + S , ! èӚ # ÆÖ. # èwº # ç ;TS.% ƒœ¶Àhüý .<# ’“ WÉ`89 + & , ; üý)Ì # %$$" # %C ! ! ") %%$P%%Q; V8&V # J02* U >V # V8TI # 2/+,;_2*2/8=+*+, : 383'-/02*'*P 3/)(=/()+, U 2*2 ! .<# " '-TS.%+98+*8*-,(2*a+98)(32383',+/2? 8*J08*+ + & , ;J08*232&'()*+,'-@8)',' U: # %$$" # %C ! ! ") %%$P %%Q; ! 8*J08*232 " + #$ , ! <8+G+\Z'0+)8 # I2/2)_ : +)G+/8 # I2/2)O0')2 1 * # 2/+,;_2*2/8= =0+)+=/2)8a+/8'*'-/02.< U 2*28*?8=+/23='P=8)=(,+/8'*'-/1' 3(HP,8*2+ U 23'-08 U 0, :F +/0' U 2*8=+98+*8*-,(2*a+98)(3'- TD.#3(H/ :F 28*.')/02)*>()' F 28*%$$Q + & , ;@8)(3_2*23 # %$$" # !Q ) ##BP#%D; + ## , ! &(,\(*2*Y # <+)2*29+O # I8)0'*2*& # 2/+,;M',2=(,+) F +/0' U 2*P 2383'-8*-,(2*a++98)(38*-2=/8'*+*?98)(3P8*?(=2?)2 U (,+/8'* '-= : /'\8*2 U 2*22] F )2338'* + & , ;J : /'\8*2_)'1/06+=/') K29 # %$$# # #% ) #B#P#BQ; + #% , ! 6+,=m*4 M # M+/8m*K M # Z()=02)O # 2/+,;L2-2=/892K.4 )2 F ,8=+/8'*+*?,+/2 U 2*22] F )2338'*8*/2G F 2)+/()2P32*38/892 8*-,(2*a+98)(3232] F )2338* U ?2,2/2?-')G3'-/02.<# F )'/28* + & , ;&@8)', # %$$C # B" ! " ") !""$P!"""; + #! , ! Z08)*'9AI # '^*+\'9+O> # j',--O # 2/+,;.<# F )'/28*'-8*P -,(2*a++98)(3?'1*P)2 U (,+/23+ FF '/'383 + & , ;&@8)', # %$$% # BQ ! C ") #Q#BP#Q%D; ./ +,-.(/0))Ì ! &'()* " ! !" "