热盐水致大鼠萎缩性胃炎血清和胃黏膜组织SOD和TAC的变化

热盐水致大鼠萎缩性胃炎血清和胃黏膜组织SOD和TAC的变化 作者：江梅，张沥，海春旭，秦绪军，陶梅，张玲霞，曹广州 【关键词】 萎缩性胃炎 Alterations of SOD and TAC in serum and gastric mucosa of atrophic gastritis rat caused by highsalt hot water 　　【Abstract】 AIM: To investigate the dynamic variation of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in the process of atrophic gastritis formation caused by over salty hot diet. METHODS: The atrophic gastritis rat model was made by feeding 150 g/L salty water of 55℃ for 12 weeks. The normal control was fed with pure water of 25℃. The rats were killed group by group every 4 weeks and the serum and gastric mucosa were kept to test SOD and TAC. Tissue slice of gastric mucosa was observed under optic microscope. The SOD was tested by improved muriatic acid hydroxylamine method, the total albumen content of stomach homogenate was tested by Lowrys method and TAC was tested by TAC kits. RESULTS: In the 12th week, the gastric mucosa in rats fed with highsalt hot water presented with typical pathological change as atrophic gastritis, but this pathological change was absent in control group. SOD and TAC in serum and gastric mucosa had a perfect timeeffect relation in hotsalt water group. With the time prolonged, the SOD and TAC decreased significantly in hotsalt water group. CONCLUSION: Active oxygen damnification plays an important precursory role in the formation and evolution of atrophic gastritis. 　　【Keywords】 atrophic gastritis; highsalt hot diet; SOD; TAC 　　【摘要】 目的: 观察过热、过咸饮食引起胃黏膜损伤以致造成胃黏膜萎缩的过程中超氧化物歧化酶（SOD）及总抗氧化力（TAC）的动态变化. : 采用55℃，150 g/L NaCl连续灌胃12 wk，制成大鼠萎缩性胃炎模型，对照组采用等量的25℃水灌胃,在此期间每隔4 wk处死一批大鼠. 将大鼠麻醉后腹主动脉取血分离血清，同时摘取胃组织，制成组织匀浆，分别测定血清及胃黏膜组织中SOD和TAC含量. SOD测定采用改良的盐酸羟胺法， TAC采用南京建成生物公司的试剂盒，总蛋白测定采用Lowrys法. 结果：与对照组相比较，经热盐水灌饲大鼠的血清和胃黏膜组织中SOD活性及TAC水平显著降低（P＜0.05，P＜0.05），并且随处理时间延长，SOD活性和TAC降低愈显著. 结论：SOD和TAC的显著改变说明活性氧损伤在萎缩性胃炎发生发展过程中起着重要的先导作用，也提示有效的抗氧化干预很可能对于预防萎缩性胃炎具有很好的防治作用. 　　【关键词】 萎缩性胃炎；热盐水；超氧化物歧化酶；总抗氧化力 　　0引言 　　慢性萎缩性胃炎是临床常见的胃部疾病，由于其与胃癌尤其是肠型胃癌的发生呈显著正相关，1978年世界卫生组织将其定为胃癌前状态［1］. 我们以人类饮食中不可缺少的两大因素食物的热度与咸度，创建了快速建立慢性萎缩性胃炎动物模型的方法，并观察从正常胃黏膜发展到萎缩性胃炎的演变过程中超氧化物歧化酶（superoxide dismutase,SOD）和总抗氧化力（total antioxidant capacity,TAC）的动态变化，以探讨活性氧损伤与萎缩性胃炎发生的关系. 　　1材料和方法 　　1.1材料 　　健康、性成熟的雄性SD大鼠44只7 wk龄，体质量200~250 g，由第四军医大学动物实验中心提供. 盐酸羟胺、Triton X100及NBT购自华美生物公司，福林酚购自鼎国生物公司，其余试剂均为国产纯. TAC试剂盒购自南京建成生物研究所. 722型光栅分光光度计为上海分析仪器总厂产品，SHZ881台式水浴恒温震荡器为江苏太仓鹿河生化仪器厂产品. 采用55℃, 150 g/L NaCl连续灌胃12 wk，制成大鼠萎缩性胃炎模型［2］，对照组采用等量25℃水灌胃12 wk.