输精管结扎术后附睾精子超微结构的变化

输精管结扎术后附睾精子超微结构的变化 :????+????????C?7???????b?????????e????(96066),??+d?:???????y?????e????(???????l?? []66??),??+d?:+????????C?7???????b(2) ???0???#56????,??+d?:???C?7?????????y?>???T ?b?????0??+?+?,q@ z<????z2z?????z??)???????????+?y?8?w???????????????????Az(z?????z?38?????????g?[?y ?0?)3??(B??:???+?8<2??,C??:???+?82??5??,D??:???+?8>5??),13???????????++J?????? (A??)z(?o?=?y???????????????????????w????,?V?????R???????????:?????/?????)??z(z????? z?A?????????w?????????M???:?????(?????????B+V(P>0.05)z(?R?????V???????????O?J:A?? +8?????????+?y?83???????????M???:(a)z:?N??????(b)z:????????(c)???O??????(d)???(?k?]?? ?????B+V(?kP<0.001),?????R???)?m?y:(a):(40.28??11.53)%??(16.80??7.93)%,(6.29?? 4.57)%,(4.63??5.06)%;(b):(35.00??14.18)%??(59.05??14.44)%,(63.43??15.23)%, (82.05??16.71)%;(c):(20.83??6.40)%??(13.60??6.78)%,(14.71??6.82)%,(9.00??7.18)%; (d):(3.89??4.44)%??(10.55??11.73)%,(15.57??9.81)%,(4.32??7.65)%z(?V???)?m?y:(a): (49.12??20.55)%??(19.95??15.42)%,(10.00??9.50)%,(5.84??9.63)%;(b):(35.00?? 14.55)%??(22.55??16.24)%,(14.71??15.78)%,(10.68??18.65)%;(d):(15.80??9.55)%?? (57.50??24.74)%,(75.29??23.90)%,(78.21??30.33)%z(?B?6?R???????????Nz:??z:?O?V?6+h?? ?#?6?????????k?]?????(?Mz(z?????z??????????+?y?8?w?????????????y?????#??,?0???Nz:??z:?O ?????b+h?????????(?Mz(?????????+?y?8????????,?(?M??????+s??,????????z( z<???i??z2z??????????+?y;z??w??;z?????;z????????? Ultrastructureofhumanepididymalspermatozoaaftervasectomyz?WENRen2qian,LIUMei2yi,YEJia2 ling,CHENQiao2er,YAOXiao2tao,TANGLi2xin.FamilyPlanningResearchInstituteofGuangdong,Guang2 zhou510600,China Correspondingauthor:WENRen2qian,Email:wenrq@tom.com z<Abstractz2z?Objectivez?Toinvestigatetheepididymalspermultrastructureaftervasectomy.z?Meth2 odsz?Duringtheoperationofvasovasostomy(38cases,testgroup)andvasectomy(13cases,controlgroup, groupA)theproximalvasdeferensfluidswerecollected.Theepididymalspermmorphologyintheproximal vasdeferensfluidswasobservedusingopticalandelectronicmicroscopes.DatawereanalyzedbySPSSpro2 gram.z?Resultsz?ThedatafromgroupAshowedthattherewasnosignificantdifferencebetweenleftand rightsideinthepercentageofnormalspermmorphology.Theresultsfrombothopticalandelectronicmicro2 scopesshowedthattherewasasignificantdifferenceinthepercentageofnormalspermmorphology(a),ab2 normalspermhead(b),spermwithneckdefect(c)andspermwithtaildefect(d)betweengroupAand groupB(<2yearsaftervasectomy),groupC(2-5yearsaftervasectomy),groupD(>5yearsaftervas2 ectomy).(electronicmicroscopes:(a):(40.28??11.53)%vs(16.80??7.93)%,(6.29??4.57)%, (4.63??5.06)%;(b):(35.00??14.18)%vs(59.05??14.44)%,(63.43??15.23)%,(82.05?? 16.71)%;(c):(20.83??6.40)%vs(13.60??6.78)%,(14.71??6.82)%,(9.00??7.18)%;(d): (3.89??4.44)%vs(10.55??11.73)%,(15.57??9.81)%,(4.32??7.65)%.opticalmicroscopes: (a):(49.12??20.55)%vs(19.95??15.42)%,(10.00??9.50)%,(5.84??9.63)%;(b):(35.00?? 14.55)%vs(22.55??16.24)%,(14.71??15.78)%,(10.68??18.65)%;(d):(15.80??9.55)%vs (57.50??24.74)%,(75.29??23.90)%,(78.21??30.33)%.Thereexistedmulti2cellorganandmulti2 formabnormalitiesintheinnerstructuresofspermheads,necksandtails.z?Conclusionsz?Theepididymal ??021??+p?+???X?A???x??2006??4?[?n27?????5z?ChinJUrol,April2006,Vol27,Suppl ?? 2001200008 :1000 :Email:wenrtom.com spermdefect(includinghead,neckandtail)aftervasectomywasdemonstratedintheinnercellorgans.The longeraftervasectomy,thehigherpercentageofspermwithabnormalitywasfound. z<Keywordsz2z?Vasectomy;z?Epididymis;z?Spermatozoa;z?Ultrastructure z?z??>??????,?????????+?y?8????????????+p?? ??????????,?????g???????+,???M???:???????? ?2,?B?N????z:+3???????????O?????????????? ?? [1,2] z(?????g?[?y?8???;??+3????????+=+??y?? ?????:???h?????????3 [3,4] z(?w?????????y?????? ???+?y????????,?y?????????????+?y???????o?? ???;+=+?,?w??????????????+???+J???=z(+J??+? ??+????????????+?y?8?w???????????????????A, ?2+????o?R???O????????+????????g?[?y???0?w?? ????????????????,?????K??+qz( ????+8???? +?z:+N???w?? ?????????+?y?81??9???I?e38??,????25?? 50??z(????????,?/+b?k+J???2,???6?????????u ?[???C?7???X?????x?????????g?[?yz(13?????7 ??2+h+?+5???????????????+?y???I?e,????24?? 38??,+J??????z( +?z:?=?y?????I ???????g?[?y?o?T???????A???A????+q???/, ?????????+?y?o?T??????+q???/z(?,??????????, ?o????????????+p??????+?????,?L???????????? ?????????w??????z(??????(A??)????18+?(?? ??10+?,????8+?)z(38?????????w?????,?n46 +?(????26+?,????20+?),?]???+?y?8+3?@???? ?)+J3??(B??:???+?8<2??,C??:???+?82??5 ??,D??:???+?8>5??)z(?,?n?????8????????+8 HamF10??????+p,37U9????+p????z(?????)???k ???V???M?????????:??????,??+q?????P??????,?o ??????+p??4%?5+???????,???R???)??z( +]z:?V???O?????)?? ?Y??WHO?,???????)?????? 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[7] ,?@?n???R???N???o???M+??w ??????+p??+?????????20%???????(?M,?o?V??+p ?????)?????(?M?????N???(?M,????,?V??????+??N ???(?M+J+gz(?R??+q?????????Y??+???+h???????( ?M?Y??,?r???y?N???(?M+J+g,?0????????z:????z:?? ?????O??,+????6???????????(?Mz(?????R???y+? ??+3?@?x?U??????,?T?O?J??????+??G?)????,???I ?e?7???7?)??,?????????+?y???????7?????>???k ?]?i??z( ?n?>???????O?J,?????????+?y?8?w???????? ?????????(?M?y?b??????,?0???]?????N??z:?O???( ?M,???]?V???????????????????(?M,?S?h???????? ?????+?y?8??????????????????????,?e?b?Y??+8 ?I?e+3?????0?????Y?????@ [7,8] ,+g???????]?e?Nz: ?B?Nz:+3???a?N????????,?N???J+?????z:????????z: ???????N???V;?????]?N?O?)??????,+3???a???P ????????????;?O??9+2???????v?A?Y??,?????? ???P?5+?z:?)?9+3????z:?????????J?v?Vz(??+??Y?? ???n+5?y???g?e??????????????z(?S?n?>???????? ?????????????+?y?8?w????????????????????,?? ?8?????????+?y?8?w??+p??????????????,?r???y ?????R?????)????????+?+J+N?????????b?????9, ???8+??????????+?y?8?w?????????????y?????# ??,?????Nz:??z:?O?????b+h?????????(?Mz( (???l:?+????+b?e???R?????????>????+????b?i??z(+A?/?? ?7????????+????????xz() ??z??9z???z??i 1z?WenRQ,LiSQ,WangCX,etal.Analysisofspermatozoafromthe proximalvasdeferensofvasectomizedmen.IntJAndrol,1994,17:352 39. 2z?WenRQ,LiSQ,WangCX,etal.Morphologicalchangesofspermato2 zoainproximalvasdeferensaftervasectomy.AsianJAndrol,1999,1: 61265. 3z?ShrepfermanCG,CarsonMR,SparksAE,etal.Needforspermre2 trievalandcryopreservationatvasectomyreversal,JUrol,2001,166: 178721789. 4z???+?+?,?.???B,?????4,?V.???????????g?[?8?7???????3???? ???;???4??.+p???I?????x??,2003,17:1842187. 5z?WorldHealthOrganization.Laboratorymanualfortheexaminationof humansemenandspermcervicalmucusinteraction.4rded.Cam2 bridgeUniversityPress,1999. 6z?YamamotoM,hibiH,YokoiK,etal.Surgicaloutcomeofmicroscopic vasectomyreversal:ananalysisof30cases.NagoyaJMedSci,1997, 60:37242. 7z??????t,???,,?;????.?I?e+3?????0?????????J??????.?R???O ??????,1998,17:4052406. 8z?????,???S??,??????,?V.+3???????0???????????????>??;>;>;> ?????N?O?)??.+p?+???X?A???x??,1997,18:48250. (?L?X???7:2005205207) (?n???A???????2) ??321??+p?+???X?A???x??2006??4?[?n27?????5z?ChinJUrol,April2006,Vol27,Supp l ?? :