.芪丹通脉片对大鼠心肌线粒体损伤的保护作用

.芪丹通脉片对大鼠心肌线粒体损伤的保护作用 垃圾车价格 www.xgcszqc.com ×?Õß?ºÍõ×ÚÈÊ??Âí????ÐÐÀû??ÂíÊÀÆ????ÂÏþÀò??????ÁÕ??Àî???ª??Ð?Ìú?Ü??Ö??? ???Ø?ü?Ê?? Üε?Í?ÂöÆ? ??????Abstract?? AIM: To study the protective effects of Qidantongmai tablet (QDTMT) on the injury of myocardial mitochondrion induced by isoproterenol. METHODS: Rats were fed with QDTMT??2 g?kg-1??for two weeks and acute myocardial ischemia was induced by subcutaneous injection of isoproterenol (ISP. 5 mg?kg-1) into rats. The content of MDA and the activity of SOD, GSHPX and SDH in myocardial mitochondrion were measured. RESULTS: In the model group, the content of MDA increased and the activity of SOD decreased significantly. Compared with those in the model group, the MDA content of mitochondrion reduced in the QDTMT group and the activity of SOD, GSHPX and SDH increased significantly ?Û( 8.9?À0.6 0) vs (5.5?À0.6) ?Ìmol? g-1, (673.2?À25.2 ) vs (536.2?À20.3) kNU? g-1, (55.3?À6.0) vs (40.7?À7.1) kU?g-1, (0.07?À0.01) vs (0.05?À0.01) kat?g-1 , P<0.01). CONCLUSION: QDTMT can alleviate the injury caused by oxygen free radicals to myocardial mitochondrion by decreasing the content of MDA and increasing the activity of SOD, GSHPX and SDH in myocardial mitochondrion. ??????Keywords?? Qidantongmai tablets; acute myocardial ischemia; mitochondrion; malondialdehyde; superoxide dismutase; glutathion peroxidase; succinate dehydrogenase ??????ÕªÒª?? Ä?µÄ?º Ì?ÌÖÜε?Í?ÂöÆ???QDTMT???ÔÒì?ûÉöÉÏÏÙËØ??Iso??ËùÖÂ??ÐÔÐÄ??È?ѪµÄ?ÀÖÎ×?ÓÃ. ?????º ?ø?óÊó?à?þQDTMT??2 g?kg-1?? 2 wk??Æ?ÏÂ×?ÉäÒì?ûÉöÉÏÏÙËØ??Iso?? 5 mg?kg-1????ÓÕµ???ÐÔÐÄ??È?ѪÄ?ÐÍ???â??ÐÄ??ÏßÁ?Ìå??Mit??ÖÐ?û?þÈ???MDA??????Ñõ??ÎïÆç??Ã???SOD?????Èë×?ÊëÄ?ýÑõ??ÎïÃ???GXHPX????çúçêËáÍÑÇâÃ???SDH??. ?á?û: Ä?ÐÍ×éÏßÁ?ÌåÖÐMDAº?Á?Ôö?Ó??QDTMT×éÏßÁ?ÌåMDAº?Á?ÏÔÖøµÍÓÚÄ?ÐÍ×é ?Û (8.9?À0.6) vs (5.5?À0.6) ?Ìmol? g-1 , P<0.01?Ý??ÏßÁ?ÌåÖÐÄ?ÐÍ×éSOD?îÐÔ?µµÍ??QDTMT×éSOD?îÐÔÉý?ß??Á?ÕßÏà?È?ÏÓÐÏÔÖøÐÔ?îÒì ?Û(673.2?À25.2) vs (536.2?À20.3) kNU?g-1 , P<0.01?Ý. QDTMT×éGSHPX?îÁ?ÏÔÖø?ßÓÚÄ?ÐÍ×é ?Û(55.3?À6.0 ) vs (40.7?À7.1 ) kNU?g-1 , P<0.01 ?Ý. QDTMT×éÏßÁ?ÌåÖÐSDH?îÐÔÃ?ÏÔÉý?ß ?Û(0.07?À0.01) vs (0.05?À0.01) kat?g-1, P<0.01?Ý. ?áÂÛ?º QDTMT?ÉÍ??ý?µµÍÐÄ??ÏßÁ?ÌåÖÐMDAº?Á???Ôö?ÓSOD, GSHPX??SDH?îÁ????õÇáÑõ×ÔÓÉ?ù?ÔÐÄ??ÏßÁ?ÌåµÄËðÉË. 垃圾车价格 www.xgcszqc.com ???????Ø?ü?Ê?? Üε?Í?ÂöÆ?????ÐÔÐÄ??È?Ѫ??ÏßÁ?Ìå???û?þÈ?????Ñõ??ÎïÆç??Ã????Èë×?ÊëÄ?ýÑõ??ÎïÃ???çúçêËáÍÑÇâÃ? ????0ÒýÑÔ ????Ñõ×ÔÓÉ?ùÊÇÔì?ÉÐÄ??È?ѪµÄÖØÒªÒòËØÖ?Ò??Û1?Ý. Ï??ûÄÚ?á??µÄÏßÁ?Ìå?ÔÑõ×ÔÓÉ?ùËðÉËÊ??ÖÃô?Ð?Û2?Ý??ËùÒÔ??Í??ý?ÔÏßÁ?ÌåµÄ?úÐ????á??????ÄÜ?ä??µÄÑÐ?????È?ÉÒÔÁË?âÐÄ??È?ѪÐÔËðÉ˵Ä?úÖÆ??ÓÖ?ÉÒÔÁË?âÒ?ÎïµÄÖÎÁÆÐ??û. ÒÔÍùÑÐ???íÃ???Üε?Í?ÂöÆ?(Qidantongmai tablets, QDTMT)ÄÜ???ÄÉÆÈ?ÐÄ??È?Ѫ?Ì?È??ËõÐ?ÐÄ????ËÀ??Î??Û3?Ý. Ä?Ç?ÉÐÈ???ÓÐ?Ø?ÃÒ??ÔÐÄ??ÏßÁ?ÌåÓ?ÏìµÄÑÐ??. ÎÒÃÇÌ?ÌÖQDTMT?Ô?óÊó??ÐÔÐÄ??È?ѪÐÄ??ÏßÁ?ÌåµÄÓ?Ïì???øÒ???ÁË?âQDTMT?ÄÉÆÐÄ??È?ѪµÄ×?ÓÃ?úÖÆ??ΪÆäÁÙ??ÖÎÁÆÐÄ??È?ѪÌá???É??µÄÀíÂÛÒÀ?Ý. ????1?ÄÁϺÍ???? ????1.1?ÄÁÏQDTMTÌáÈ??þ?à?É?Û???ÆÜÎ30 g??µ??Î30 g??µ??é15 g???ðÖ?10 g??ºì??30 g???ËÖÆ?Á1 g?É?ÛÏàµ?ÓÚÉúÒ?2.86 g????Î???Ò?ÔºÌá????Ìñ?ûÐÄ??Diltiazem????Õã?,ÑÇÌ?ÖÆÒ????öÆ???ÑÎËáÒì?ûÉöÉÏÏÙËØ??Isoprenaline Hydrochloride????ÓÉÉϺ?Ìì?áÖÆÒ????öÆ?. 752C×ÏÍâ?Ö?â?â?È?Æ??ÉϺ?µÚ?þ?ÖÎöÒÇÆ???????GL20AÈ?×Ô???ßËÙÀä??ÀëÐÄ?ú??ºþÄÏÒÇÆ?ÒÇ?í×Ü??ÀëÐÄ?ú??????802ÀëÐÄ?ÁµíÆ???ÉϺ?ÊÖÊõÆ?е??????LMS2BÐÍ?àµ?ÉúÀí?ÇÂ?ÒÇ???É??ÒÇÆ?????. ????1.2????ÓÃSD?óÊó32Ö????ÆÐÛ?æÓÃ??ÌåÖÊÁ?250~280 g??ÍêÈ?Ëæ?úµÈ?ÖΪ4×é?ºÕý???ÔÕÕ×é??Control???º?øÓèÉúÀíÑÎË?3 mL?kg-1??Ã?ÈÕ?à?þ2?Î??Á?Ðø14 d. ÓÚ15 d??Æ?ÏÂ3??4µã×?ÉäÉúÀíÑÎË?5 mL?kg-1?? 24 hºóÏàÍ?????ºÍ?ÁÁ?×?ÉäÕôÁóË?1?Î??Ä?ÐÍ×é(Model)?ºÓÃÍ?Ñù?????à?þÉúÀíÑÎË?3 mL?kg-1. ÓÚ15 d??Æ?ÏÂ3??4µã×?ÉäÒì?ûÉöÉÏÏÙËØÒº??Iso?? 5 mg?kg-1????24 hºóÏàÍ????????ÁÁ?×?ÉäIsoÒ??Î??QDTMT×é(QDTMT)?ºÓÃÓëÄ?ÐÍ×éÍ?Ñù???????à?þÓÃÕôÁóË?Åä?É3 mL?kg-1µÄQDTMTÈÜÒº2 g?kg-1??×?ÉäIso. ÑôÐÔ?ÔÕÕ×éÌñ?ûÐÄ?ºÓÃÓëÄ?ÐÍ×éÍ?Ñù???????à?þÓÃÕôÁóË?Åä?É3 mL?kg-1µÄÌñ?ûÐÄ5 mg?kg-1??×?ÉäIso. ÐÄ??ÏßÁ?ÌåMDAµÄ?â???ºÈ?100g?L-1µÄÏßÁ?Ìå0.2mL????ÄÏ?????ÉÉúÎï???ÌÑÐ??Ìá??µÄMDA?â??ºÐÌá???????øÐÐ?â????µ?Î?ÓÃ?Ìmol? g-1?íÊ?. ÐÄ??ÏßÁ?ÌåSOD, GSHPX?îÐÔ?â????È?10 g? L-1µÄÏßÁ?Ìå50 ?ÌL????ÄÏ?????ÉÉúÎï???ÌÑÐ??Ìá??µÄSOD?â??ºÐÌá???????øÐÐ?â????µ?Î?ÓÃkNU?g-1?íÊ?. È?100 g?L-1µÄÏßÁ?Ìå0.2 mL????ÄÏ?????ÉÉúÎï???ÌÑÐ??Ìá??µÄGSHPX?â??ºÐÌá???????øÐÐ?â????µ?Î?ÓÃkU?g-1?íÊ???ÐÄ??ÏßÁ?ÌåÖÐSDH?îÁ?µÄ?â???º?ù?ÝÄÏ?????ÉÉúÎïÑÐ??ËùÌá??µÄSDH?îÁ??â??ÊÔ?ÁºÐ?â??. µ?Î?ÓÃkat?g-1?íÊ?. תÌùÓÚ 233ÍøÐ?ÂÛÎÄÖÐÐÄ ÉÏÒ?Ò?1 2ÏÂÒ?Ò? 垃圾车价格 www.xgcszqc.com ÏÂÒ?ƪ?ºÎ???ÊÐÄÐÄÐÍ?ÐÔÐÔÐÐΪÈËȺHIV/Ã????ÐÈ?×??ö??Ó?ÏìÒòËØ?ÖÎö ????Í??ÆÑ???Àí?ºÊµÑéÊý?ÝÒÔx?Às?íÊ???×é?ä?ùÊý?È?ÏÓÃ???î?ÖÎöÒÔ??LSD???àÖØ?È?Ï. ????2?á?û ????Ä?ÐÍ×éÏßÁ?ÌåÖÐMDAº?Á?ÏÔÖø?ßÓÚÕý???ÔÕÕ×é??P<0.01????QDTMT×é??ÑôÐÔ?ÔÕÕ×éMDAº?Á?ÏÔÖøµÍÓÚÄ?ÐÍ×é??P<0.01????Ä?ÐÍ×éÏßÁ?ÌåÖÐSOD?îÐÔ?µµÍ??QDTMT×é??SODµÄ?îÐÔÉý?ß??Á?ÕßÏà?È?ÏÓÐÏÔÖøÐÔ?îÒì??P<0.01??. Ä?ÐÍ×éÏßÁ?ÌåÖÐGSHPX?îÁ?Éý?ß??µ?Óë?ÔÕÕ×éÏà?ÈÎÞÏÔÖøÐÔ?îÒì??P>0.05????SDH?îÁ?µÍÓÚÕý???ÔÕÕ×é??µ?ÎÞÍ??ÆÑ?ÒâÒå??QDTMT×é??ÑôÐÔ?ÔÕÕ×éGSHPX??SDH?îÁ?ÏÔÖø?ßÓÚÄ?ÐÍ×é??P<0.05????QDTMT×éÓëÄ?ÐÍ×éÏà?ÈÓÐÏÔÖøÐÔ?îÒì??P<0.01????ÑôÐÔ?ÔÕÕ×éÓëÄ?ÐÍ×éÏà?ÈÒàÓÐÏÔÖøÐÔ?îÒì??P<0.05????ÐÄ??×éÖ?ÖÐÄ?ÐÍ×éSDHÓëÕý???ÔÕÕ×éÏà?È?ÏÎÞÏÔÖøÐÔ?îÒì??P>0.05?????øQDTMT×éÓëÄ?ÐÍ×é?È?ÏÓÐÏÔÖøÐÔ?îÒì??P<0.05??. ?????í1QDTMT?ÔMDAº?Á???SOD??GSHPXºÍSDH?îÐÔµÄÓ?Ïì??ÂÔ?? ????Tab 1Effect of QDTMT on changes of MDA content and activity of SOD, GSHPX and SDH??ÂÔ?? ????3ÌÖÂÛ ?????ÚÐÄ??ÊôÓÚÖÐÒ?Ñ?µÄ??ÐØ?Ô??ÐÄÍ???ÐØÍ???ÕæÐÄÍ???ØÊÐÄÍ?µÈ?????ë. Ö?ÒªµÄ??Àí?úÖÆΪ??Ðé?êʵ. ËùÒÔ??ÖÐÒ?ÖÎÁÆÇ?µ?ÒÔ??ΪÍ?????ÖÐÔ?Í???Í????æÊ????ïµ?ÆøÓ?ѪÐÐ??ѪÂöÍ?Àû. ÏÖ?úÒ?ÀíÑÐ??Òà?íÃ???ÒæÆøÒ?Îï?ÔÐÄѪ?ÜϵÍ?ÓÐ?ÏÇ?µÄµ??Ú×?ÓÃ??ÄÜÔöÇ?ñ????á??ÄÜ??ÔöÇ?ÐÄ??ÊÕËõÁ????µµÍÐÄ??ºÄÑõÁ??Û4?Ý???îѪ??ðöÒ?Îï???ßÓÐ?ÄÉÆѪҺÁ??ä??Î?Ñ,????À?ÕÅѪ?Ü??Ôö?Ó?ÚÂöÁ?Á?µÄ×?ÓÃ??Òò?øÄÜ?ÄÉÆÐÄ??È?Ѫ??È?Ñõ×?Ì????ÀÖ?ÌåÄÚѪҺÄý?á???µµÍѪҺÕ??í?È. ????QDTMTÊÇÔÚ×æ?úÒ?Ñ?ÕâÖÖÆøѪÏà?ØÀíÂÛµÄÖ?µ?ÏÂ???áºÏÁÙ??ʵ?ù??ÏÖ?ú?ÆÑ?ÀíÂÛÑÐÖƵÄ??ÖÐÒ?????ÖÆ?Á. ?ÃÖÆ?ÁÓÉ?ÆÜÎ??µ??Î??µ??é??ºì?????ðÖ?×é?É. ÑÐ????ÏÖ???ÆÜÎÔíß?ÄÜ???æÐÄ??º?Ë?Á????µµÍÐÄ??ÈéËáÍÑÇâÃ?ºÍ??Ëá??Ã?º?Á????ÄÉÆÐÄ??ÄÜÁ??úÐ???????Ï??û?á??ÌØ?ðÊÇÏßÁ?Ìå?á????Ìá?ßÏ??ûÄÚSOD?îÐÔ???õÇáÑõ×ÔÓÉ?ù?ÔÐÄ??Ï??ûÄ?µÄËðÉË?Û5?Ý. µ??Î??ÆäÓÐÐ??É?Ö?É?º?â?óÊóÐÄ??È?ѪÔÙ?à×?ºóSODºÍGSHPX?îÐÔÏÂ?µ???º?â?óÊóÐÄ??È?ѪÔÙ?à×?ÒýÆðµÄÐÄ??ÏßÁ?ÌåÄ?Á???ÐÔÏÂ?µºÍÖ?ÖÊ?ýÑõ???Û6?Ý. ???á?û?íÃ???QDTMT?ÉÔö?ÓÐÄ??ÏßÁ?ÌåSOD??GSHPX?îÐÔ???µµÍÏßÁ?ÌåMDAº?Á???ÒÔÓÐÐ?µØÏû?ý?òÒÖÖÆÑõ×ÔÓÉ?ùµÄÆÆ?µ×?ÓÃ???ïµ??ÀÖ??ÚÐÄ??µÄÄ?µÄ. ???????Î??ÎÄÏ×?? 垃圾车价格 www.xgcszqc.com ?????Û1?Ý Wu JX, Wang YX, Niu RX, Zhou YG, Liang N. protective effects XML on the rats with myocardial injury induced by isoprenaline ?ÛJ?Ý. 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Zhongguo Zhongxiyi Jehe Zazhi??Chin J Intergr Tradit Western Med??, 1999;19 (8):481-483. ?????Û6?Ý Lin LZ, Wu CL, Lu Z, Huang YR, Zhuang JG, Zhou ZN. The studies on protective effects of SM against myocardial hypoxia/reoxygenati on injury ?ÛJ?Ý. Zhongguo Yingyong Shenglixue Zazhi (Chin J Appl Pharmacal) , 2000;16 (4):324-326.תÌùÓÚ 233ÍøÐ?ÂÛÎÄÖÐÐÄ ÉÏÒ?Ò? 1 2ÏÂÒ?Ò? ÏÂÒ?ƪ?ºÎ???ÊÐÄÐÄÐÍ?ÐÔÐÔÐÐΪÈËȺHIV/Ã????ÐÈ?×??ö??Ó?ÏìÒòËØ?ÖÎö