ABCG4在肺鳞癌、腺癌中的表达及临床意义

ABCG4在肺鳞癌、腺癌中的表达及临床意义 ABCG4在肺鳞癌、腺癌中的表达及临床意 义 ABCG4?Q?????J0A???J?v?????????b???o?> ?I????,???o??,???o??,??????,??0???,?]????,?0????,??????,?p???? TheexpressionofABCG4proteinanditsclinicalsignificanceinthesquamouscelllung cancer,lungadenocarcinoma ZHANGZhi-pei,LIXiao-fei,WANGXiao-ping,ZHOUYong-an,XUHong,CHENDe-feng,TIANYu-tu, ZHAOYa-chao,CHENGQing-shu DepartmentofThoracicSurgery,TangduHospital,FourthMilitaryMedicalUniversity,XiIuan710038,China. 0nAbstract0f0?Objective:ToinvestigatetheexpressionofABCG4proteininsquamouscelllungcancer,lungadeno2 carcinoma,andanalyzerelationshipofABCG4expressionalrateinthesecancerwiththepathologicalgradeandclini2 calstages.Methods:TodetecttheexpressionrateofABCG4insquamouscelllungcancer,lungadenocarcinoma withimmunohistochemicalmethodandimmuno-fluorescentmethod,andthelocationoftheproteinswereobserved underlightmicroscopeandconfocallaserscanningmicroscope.Results:TheABCG4proteinwasover-expressed withthe67.3%and81.4%totalpositiveratesinsquamouscelllungcancer,lungadenocarcinomarespectively,and betweenthetwokindsofthecancerstherewasasignificantdifference(P=0.001)forthepositiveexpression.The positiveexpressionratesofmoderatelydifferentiatedandlowdifferentiatedsquamouscelllungcancerwere41.7%, 92.0%respectivelywithaextremelysignificance(P=0.000)fortheircomparison;andthepositiveexpressionrates ofmoderatelydifferentiatedandlowdifferentiatedlungadenocarcinomawere76.7%,92.3%withalsoasignificant difference(P=0.033)fortheircomparison.Therewerealsosignificantdifferenceamongthesquamouscelllung cancerandlungadenocarcinomafortheclinicstagesrespectivelybyKruskal-Wallistest,withthePvaluesbeing 0.039,0.048respectively.TheABCG4positiveexpressionwasmainlylocatedintheplasmamembraneandcyto2 plasm.Conclusion:TheABCG4proteinareoverexpressedinsquamouscelllungcancer,lu ngadenocarcinoma,and theexpressionofABCG4proteiniscorrelatedwithpathologicalgradeandclinicalstage,whichwouldbebenefitfor furtherstudyofABCG4intumoranditschemoresistance. 0nKeywords0fnonsmallcelllungcancer(NSCLC);ABCG4;drugresistanceprotein ModernOncology2008,16(11):1901-1903 0n????0f0?????:???/?n??????ABCG4?Q?????J0A???J?v??????,?,???????????i???,?,?????b???,?L?????? ??,?6?d??ABCG4?Q???U???????n???????D???????)0B????:???Q?????????l??EnVinsion??0A???????>???? ?/ABCG4?Q?????J0A???J?v???????????>?????E?P?????????????>????0B????:ABCG4?Q?????J0A???J?v?? ????,???J?%?.?D??67.3%0A???J?%?.?D???681.4%,?U?4???B?D?P??(P=0.001);?v?,?l0A???,?l???J?.?D ???,?F?641.7%0A92.0%,???B?D???w?P??(P=0.000);???J?v?,?l?.?D??76.7%0A???,?l???J92.3%,???B ?D?P??(P=0.033);???J?????J???G?b???,?L??Kruskal-Wallis???????????B?D?P??,P?k?,?F?60.0390A 0.0480BABCG4?%???????Q?????x?????Q?v0B????:ABCG4?Q?????J0A???J?v??????,???????????i???,?, ??0A?b???,?L?e??,?6???????d??ABCG4?Q???U?v?????????????n?????Q????????????0B 0n???7??0f???o???????J;ABCG4;?n?????? 0n?v???,????0fR734.20?0?0?0n???7?T???A0fA0?0?0?0n?????l??0f1672-4992-(2008)11-1901-03 0n?:?=???L0f0?2007-12-24 0n???????L0f0?2008-04-16 0n????????0f0??????A?%??????(2004K13-G7) 0n???4????0f0??M?????%?D???n???%???????l??,????0?????0? 710038 0n???4????0f0??I????(6),?z,??????????,??????,????,?%?? ???????U???,?,????,???????J?n???D?d??0B 0n?m?????40f0??p????(5),?z,??????,????,???????h,?????? ?J???)???b???d??????0B 0?0????????J??????0A???????n???x?[,???V?z?D???D???U ?/??,?V???D???D???U2-3??,?d?????????????l,?Y???? ???????f?????????? [1] 0B???????J?????m???Q?m???????h ????,???v?l???Q???J?????v???e?????0??,?5???J???l?? ???]???1?????????3?Y?????????? [2] ,ABC(ATP-binding cassette)?m?????????????????????l?????l?????n?????%?? ???Q [3] 0BBG?1B?D???????????vG?????M?y?n ??,????????????X????(LXR)??RXR(X) ?C???[??,???/BG?????i?C???B?Q?l???????W?e??,?5 ??1091?????????U?%??0?2008??11?h0??M16???M11?L ?? 194- 190- AC4AC74 retinoidreceptor AC4 [4] 0BABCG4?????Q???U?? ???o???????J?????,??0A?,???v?????????i?n???????D???y ??????,?M?????Q???????l0A???????>????ABCG4?Q???o?? ?????J????(?G???,?,??,?,??,?b???,??)????,?6?d??AB2 CG4???Q???o???????J????0A?n???D????????0B 10??????i???? 1.10?????0? ?:??2006??10?h-2007??11?h?M?????%?D???n???% ?????l???????D?????J0A???J?????/?1?T???T?M92??,?z66 ??,??26??,????33??-77??;?)?e?T?M?[?Q10%???d???? ????,???3?b0A?o?i,?w?????X?4??0B?3???,?????????J???? ???,?,??,?1?i???J?b???,??0B ABCG4?????????v??????????abcam???5???l??????, EnVision TM ???????l???2???v????????????????DAKO?? ??,???????>?????v??SantaCrus?L?????1????0B 1.20????? 1.2.10????????l0??T?M??5C?m?????T?[,?,?F??HE???? ?????l????0B???????l???????Q?U????(EnVision TM )0B???? ???p:?T?[?3?X???b,?=?I???l,3%???D?l?W?A??,?Q?????? ?a???????J???????s,????,10%???)?4?A??,??????4XC?? ?H;??????????37XC?s?S,?Q?????b???g????,???????????? 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Mann-WhitneyU????????,???J?????J?b???,?????Q?m?? ?>???K??Kruskal-Wallis????????0B 20?????0? ???????l?P?6ABCG4?.?D?????%?????????????x???? ?Q?v(??1),???????>?????%???????Q?????x(??2)0B???J ?%???.?D??????67.3%0A???J?%?.?D???681.4%,?U?4???B ?D?P??(P=0.001),????1;?v?,?l0A???,?l???J?.?D???,?F ?641.7%0A92.0%,???B?D???w?P??(P=0.000);???J?v?, ?l?.?D??76.7%0A???,?l???J92.3%,???B?D?P??(P=0.033),?? ??20B???J?????J???b???,?L??Kruskal-Wallis?????????? ?B?D?P??,P?k?,?F?60.0390A0.048,????3-??40B ??2091??MODERNONCOLOGY,Nov12008,VOI116,NO111 ?? 10????J?????J??ABCG4???????B ?,??n ?????.?D?6?? (-)(+)(++) ?.?D?? (%) ???J491627667.3U=647.5 ???J438132281.4P=0.001 ??20????J0A???J?s?r?,????ABCG4???????B ?,??n ?.?D?6?? (-)(+)(++) ?.?D?? (%) P ???J49 0??v?,?l141041.7U=119.0 0????,?l217692.0P=0.000 ???J43 0??v?,?l7111276.7U=121.5 0????,?l121092.3P=0.033 ??30????J?s?r?b???,?LABCG4??????0?n ?,?? ABCG4?????.?D?6?? (-)(+)(++) ?h?? X?1607C?2 =8.4 X?a2103P=0.039 X?b1216230 X?a1449 ?h??1627649 ??40????J?s?r?b???,?LABCG4??????0?n ?,?? ABCG4?????.?D?6?? (-)(+)(++) ?h?? X?1225C?2 =11.18 X?a2103P=0.048 X?b581023 X?a0246 X?b0055 X?0011 ?h??8132243 30?????0? ?/?1?T?????J?1?????????/??,?5???D???????4?3???? ???3???/?1?6?m0B???????J?????m???Q?m???????h????,?? ?v?l?????e?????0??,?5???J???l?????]???1?????????3?Y ??????????,???R?M?????1???U???????l?????????L?n?? ?D [2] 0B?)??,???U???n???D???n?????????n?????????:?1?? ?U?????d????????,???1?????y???l?l?????????????????/ ??0B ???o???????J???n???D???w?s?2,???????????????Q?? ?,???v?????7???Q,????????????????,???vABC(ATP- bindingcassette)?m?????????????????????l?????l?????n ?????%?????Q [3] 0BABCG4?1ABC7?D???????????vG???? ?M4?y?????n??,ABCG4???f?314?y?l?P?????n,10?l?P ??(2-70A10-13)?1?????6;?????????x?w300bp???eTA2 TA?1????0B??????????????????11q23.3?x,?????% ??12.7kb,???????????????i?????????B?Q???W?e?? [4] 0B ABCG?????n???3???yABCcassette???n,?,?6???w?????? ?h??(NBD)??6?y?8?????????????X??(TMD),???e?+?y?? ?????????D,???e???n???????l?m?????????e????????, ABCG4?????h?n?r????????,???????n?B?????? [5] 0B ABCG4?,?C???????d???K?????f0BOldfieldS?d?????6 ABCG4??ABCG1?,?C????,?%???Q?f???k???????????v?? ??,?3???iABCG1???r???DABCG4?Q??0A????0A??0A?????, ?C?m?s??????,?e?????????d?? [6] 0BYoshikawaM????AB2 CG4?Q?o?I?????v???????1?e???B??,?I?v???f0A?P0A?k???? ????????,???????f0A?P0A??0A???w???????e??????????,???v ?e???????m?s???? [7] 0BFujiyoshiM?Q?D?I?v???JRT-PCR ??????ABCG4?Q?f0A?k?e?????v??????,?r?Q?f0A?P0A??0A?? ?????vWesternblot?????/?0 [8] 0B????,ABCG4?Q???????? ???,?C?m?y?i??,?e?????????d??0B ???U???????vABCG4???????????m?y???d??????,?M ?????P?6?????J0A???J???e????????????(67.3%0A81.4%), ?U?????J????????,?U?4???????B?D?P??(P?k?60.001);?r ???J???,?,??0A???J???,?,?????????????e?P?????B,?J??0A ???w????????????(????);?r??,?b???,????Kruskal-Wal2 lis???????????e???B0B???iABCG4?Q?????J0A???J?v???? ??,?5???J?????J??????,?????????????i???,?,??0A?b???, ?L?e??0B?r?b?????????Q???J?????J???n????????,???d?n ???p???????i???U???D?p???e??0B ABCG2?????????l???B?Q??/?????????Q,?r???n???D ???i?? [5] 0B?)??,???f???N?????????i?e?,?3????ABCG4 ?Q???U?v???????????????????????e?l?????Q0B?e?????? ?e???????????????i0B 0n???C???70f [1]0????=??.2002-2004???x???B???)?6???J???????????????, ??[J].?x?????8?2??,2006,18(2):60-61. 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