酮胺氧化酶法测定血清糖化白蛋白

酮胺氧化酶法测定血清糖化白蛋白 (???y???L???<?????????P?L??1(10730) H2????H4???????????[?3???o??????(CSl,)???M??????????????rtrt?????p?o????????????H1?????)?????????? ??GSP?????????P?????????????p?P?VN???????????????????w?????????1?p????H1????????????GSP?????????V????CV =1?V24?3?n???_?]??4?????,?n???p?0???????P????1845tH=mol?=L?n???w???v98?V9?3?n???7????????45mmol?=L?P?????r340timol?=L?P?9 ???l?136mmol?=L?P?????[??500tnnol?=L?Pm?Y1?V8fuL???D?????V?K????H1?????g?Y?????p?o????????GSP???l???1H(????H1 ???p?0?????P???w?????P???????????v?P???_?]?????????P?B?O??????GSP?????l????H1 H2???b??H4???7?????o?????????”?? TheGlyPromethodformeasurenlentofGlyeatedSel’unlproteins(13SP)LiYilong?PWangMeng(D???c?z??lt ofClinicalLaboratory?PB??i??Hospital?PB??ilIg?P100730?PChina) IAbstract]ObjectTostudyalleglymeticmethodforGlycated8e?=llln proteins?VMethodTostudythemethodofindudingprecisionH(stable andaeeuraneyandcomparewiththemethodofNB’r?VResultsTheGlyproassayhasexeeUentwithinrtrimandbetweenrtrllllprecision?Pwithall samplesgivingCVsof<1?V24?3?VTheGlyProassayislinearupto1845??nogL?nanditsreagentcallbestableforfourweeks?ntherecovery (?3)is98?V9?3?nnosignitieantinterenceupto45mmol?=LofGlucose?n340tlmol?=LofBilirubin?n36mmol?=LofTriglyeeride?n5COmnol?=ULof Ascorbieacid?n1?V89?=irtofHemolysis?VThemethodiseonlpltgisOlrltoN???Pr=0?V8?Pa=??76?V5?Pb=1?V26?VC??Icll?6?)Theexperimentdata showthatthemethd?tofGlyProtodeteetetheGlyeatedsel’umproteins(c,sP)ishi??precision?Pspecificity?VIAne??rityrecoveryandantirtinterrt fereneestotheAseorbicacidBilinlbinH(HemolysisH(GlAaH(?3gI?8deareminfluence?VItris??}lebestmethod??0deteeteGlye,afed?r proteins(GSP)inclinic?V H2KeywDr??H4Gluefee?nGlyeated,?Ul”Ulmproteins?nDiabetes ?????[?c???7???5??????H(????H(?????????6?? ?r???????P?N?????????r???”????????????f?H1?? ?7???V?[?3???????B???o?[?3?????P?????????o?? ???P???c90?3?V?[?3???????????D189?????Y???? ?S?P???2???)?????????????P?????????k???????P?+ ???o?[?3?????????????v??????????H1?I???????? ???????y?v17rt20?g?P???????o?[?3?????????r?? ????2??3???????????[???O??[2jH1?N???????[?? ???????????P?????v???????”?????y?????w?????W ?????????C?CH1 ???????????o?[?3???????????l?6?????????? [d??PLC]H1?n????????(N??)[5]H1I-IPI_12???????p???P ???????-?2?P?????1?j???????+?N?1?S?O?????YH1 NBT???5???????1?p???Y?????P?????p????H1???? ?P????genzyme?????????????p?o????????GSP?? ???P???????G?6 1?????V?? 1?V1?????????_????7170?????????y?o???6?? ??gen??ne??????????GSP???_??(?????60910)?6?d ????(?????60907)?6???k?Y??(?????61041)?n???k?Y?? (?????60908)?P?D???????????v????????l5|H1 1?V2?R?D?????L???<???????????????)???”?? ???????????????3?[?3?C?DH1 1?V3???????)?????????? ?????_?v?????_???2?P1???_?M?l??????K?Y ?????o?R?D?P?2?????K???o????????H12???_?c?? ???????=????rtrt???o?Y?????p?o??(KAO)???1?p ?p?o???7???V???Y???????????????b?P?P???lH202 ?y?2?PH202?V?K???????Y???p?o?????????Y?G?y?2 ?K?????P?????????+?V???o???????2????H1 ???o??????H=?m?????o?????????? ???o??????????H=???????Y????+11202 H202+?K???_H=?m?h????????+H20 ???????????6?????v????7170???y?o???n