脊髓PSD-93蛋白和nNOS在颈7脊神经压迫大鼠神经病理性疼...

脊髓PSD-93蛋白和nNOS在颈7脊神经压迫大鼠神经病理性疼... 脊髓PSD-93蛋白和nNOS在颈7脊神经压迫大鼠神经病理性疼痛中的作用 中南大学湘雅医院麻醉科 410008 黄长盛 王锷 郭曲练 颜璐璐 王懿春 [摘要] 目的 观察脊髓PSD-93蛋白和nNOS在颈7脊神经压迫大鼠神经病理性疼痛中的作用。 方法 雄性SD大鼠32只，随机分为4组(n,8):C组(假手术，生理盐水组);N组(C7脊神经压迫，生理盐水组);M组(C7脊神经压迫，PSD-93误义寡核苷酸10μg组);A组(C7脊神经压迫，PSD-93反义寡核苷酸10μg组)。N、M、A组大鼠采用60g微血管夹压迫大鼠右侧C7脊神经15min制作神经病理性疼痛模型，C组大鼠做假手术，各组大鼠在做模型同时鞘内置管至颈膨大处。术后当天即开始给药，每日一次，连续4天。于术前2d和术后1、3、5、7d测机械性痛阈和热痛阈，术后7d处死大鼠取C7段脊髓，通过免疫组化方法测定神经压迫侧脊髓PSD,93蛋白和nNOS，比较这两个指标在脊神经压迫以及鞘内注射PSD-93寡核苷酸后的表达变化。 结果 与术前相比较，N组和M组术后压迫侧前足机械性痛阈降低，热痛阈缩短(P<0.05)，C组术后痛阈无明显改变(P>0.05)。A组术后压迫侧前足机械性痛阈和热痛阈无明显降低，与N组和M组比有统计学差异(P<0.05)。术后7d，N组和M组压迫侧脊髓PSD-93蛋白和nNOS表达增加，与C组相比有统计学差异(P<0.05)，A组压迫侧脊髓PSD-93蛋白和nNOS表达增加不明显， 与N组和M组相比有统计学差异(P<0.05)。对于所有大鼠，脊髓nNOS 和PSD-93蛋白的表达成正相关(r=0.86, p<0.001)。 结论 脊髓 PSD-93蛋白和nNOS与C7脊神经压迫后大鼠神经病理性疼痛的发展 有关，nNOS在神经病理性疼痛中的作用可能受到PSD-93蛋白的调节。 [关键词] PSD-93蛋白;神经型一氧化氮合酶;脊神经;压迫 Effects of spinal PSD-93 protein and nNOS on the development of neuropathic pain following rats C7 spinal nerve compression Huang Chang-sheng, Guo Qu-lian, Wang E, Yan Lu-lu, Wang Yi-chun. Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China [Abstracts] Objective To evaluate the Effects of spinal PSD-93 protein and neuronal nitric oxide synthase (nNOS) on the development of neuropathic pain after rats C7 spinal nerve compression. Methods Thirty two male SD rats were randomly divided into 4 groups (n=8 each): group C sham surgery + NS 10ul; group N C7SNC + NS 10ul; group M C7SNC + mismatch oligonucleotide ( MS ODN) 10μg; group A C7SNC + AS ODN 10 μg. C7SNC was produced by placing a microvascular clip, which has a force of 60 grams, on the right C7spinal nerve of rat for 15 minutes. All of rats were performed cervical intubation(IT) immediately after C7SNC. After finishing the surgery, normal saline or AS ODN or MS ODN was injected IT once a day for 4 consecutive days. Threshold to noxious thermal and mechanical ststimuli was measured before surgery (baseline) and on the 1, rdththth3, 5 and 7 day after surgery. On the 7 day after surgery, all of the rats were killed and the C7 spinal cord was removed for determination of the PSD –93 protein and nNOS expression stby the method of immunohistochemistry. Results During the 1 thto the 7 day after surgery, the threshold to mechanical and thermal stimuli on the ipsilateral forepaw was significantly lower in group N and M than in group C(P<0.05), but the threshold in group A was not as low as in group N and M(P<0.05). On the th7 day after surgery, the expression of PSD-93 protein and nNOS on the ipsilateral C7 spinal cord was significantly higher in group N and M than in group C(P<0.05),but the expression in group A was not as high as in group N and M(P<0.05). There was a positive correlation between the expression of nNOS and PSD-93 protein. Conclusion Spinal PSD-93 protein and nNOS may play a role in the development of neuropathic pain following rats C7 spinal nerve compression. The effects of nNOS on neuropathic pain may be modulated by spinal PSD-93 protein. [Key words] PSD-93 protein; neuronal nitric oxide synthase; spinal nerve; compression