Wnt信号在上皮干细胞和黑素细胞干细胞中的协同激活启动了色素性毛发再生

Wnt信号在上皮干细胞和黑素细胞干细胞中的协同激活启动了色素性毛发再生 Wnt信号在上皮干细胞和黑素细胞干细胞 中的协同激活启动了色素性毛发再生 ? l388? [3] [4] [5] [6] [7] 2006,22(7):1402—1405.[8] KushibikiT,Nagata—NakajimaN,SugaiM,eta1.Deliv- cryofplasmidDNAexpressingsmallinterferenceRNAfor TGF一8typeIIreceptorbycationizedgelatintoprevent interstitialrena1fibr0sis[J].JContm1Release,2005,105[93 (3):318,331. SatoM,MuragakiY,SaikaS,eta1.Targeteddisruptionof TGF—p1/smd3ig"a1i"gpmecagai"r."1"b". 10]l ointerstitialfibrosisinducedbyunilatera1ureteralobstruc一 tion[J].JClinInvest,2003,112(10):1486—1494. ArnoldJN,SaldovaR,HamidUM.eta1.Evaluationof theserumN—linkedglycomeforthediagnosisofcancer andchronicinflammation[J].Proteomics,2008,8(16):r111 3284—3293. MaupinKA,SinhaA,EugsterE,eta1.Glycogeneex— pressionalterationsassociatedwithpancreaticcancerepi-『l2] thelial—mesenchymaltransitionincomplementarymodel systems[J].PLoSOne,2010,5(9):e13002.[13] NakamuraT,AndoA,TakaqiH,etal|EOS1,whosedele— tionconferssensitivitytooxidativestress,isinvolvedinN — glyeosylationinSaccharomycescerev~iae[J].Biochem BiophysResCommun,2007,353(2):293—298. UozumiN,YanagidaniS,MiyoshiE,eta1.Purification andcDNAcloningofporcinebrainGDP—L—Fuc:N—a- cetyl—B—D—glucosaminideod—}6fucosyltransferase [J].JBiolChem,1996,271(44):27810—27817. TakahashiM,KurokiY,OhtsuboK,eta1.Corefucose andbisectingGlcNAc,thedirectmodifiersoftheN—gly— Callcore:theirfunctionsandtargetproteins[J].Carbo? hydrRes,2009,344(12):1387—1390. KondoA,LiW,NakagawaT,eta1.Fromglycomicsto functionalglycomicsofsugarchains:Identificationoftar- getproteinswithfunctionalchangesusinggenetargeting miceandknockdowncellsofFuT8asexamples[J].Bio— chimBiophysActa,2006,1764(12):1881—1889. LiuY.Newinsightsintoepithelial—mesenchymaltransi- tioninkidneyfibrosis[J].JAmSocNephrol,2010,21 (2):212—222. EfstratiadisG,DivaniM,KatsioulisE.eta1.Renalfibro. sis[J].Hippokratia,2009,13(4):224—229. WangX,InoueS,GuJ,eta1.DysregulationofTGF—B1 receptoractivationleadstoabnormallungdevelopmentand emphysema——likephenotypeincorefucose——deficient mice[J].ProcNatlAcadSciUSA,2005,102(44): 15791—15796. Wnt信号在上皮干细胞和黑素细胞干细胞中的 协同激活启动了色素性毛发再生 器官再生有赖于多种干细胞或前体细胞有组织,适时地协作.哺乳动物的毛囊(hairfollicle,HF)中含有几种干细胞群,可 作为剖析不同细胞相互协作的模型.在HF膨大部(bulge)和次级毛芽(secondaryhairgerm,sHG)内,黑素细胞干细胞(mela. nocytestemcells,McSCs)与上皮干细胞(epithelialstemcells,EpSCs)有密切的相互作用,两者一同激活,并分化为再生的色素性 ,这种干细胞协同行为背后的机制尚未被阐明. 毛发.然而 运用特异性靶向McSCs和EpSCs的转基因小鼠模型,Rabbani等发现,在小鼠毛发生长初期,sHG内McSCs和EpSCs共激 活Wnt信号;McSCs中Wnt信号的强制激活诱导了McSCs的分化和未成熟毛发的灰化(graying);B—catenin在McSCs分化中 是必需的;在毛发生长初期,EpSCs表达的Wnt配体能够激活邻近黑素细胞的Wnt信号;B—catenin在EpSCs内的稳定表达促 进黑素细胞的扩张,且是通过Edn受体B信号介导的.总之,Wnt信号的激活控制McSCs分化为产色素的黑素细胞,在EpSCs 中不仅促成HF形成,而且在毛发再生中调节McSCs的增殖. 以上结果说明,Wnt信号通路作为关键通路偶联了McSCs和EpSCs的行为,可能是异型干细胞群协同合作,再生复杂器 官的机制 Cell,2011,145(6):941—955(罗森)