2011——(2.85)榭皮素抗氯化镉诱导的鸡卵泡颗粒细胞氧化损伤

2011——(2.85)榭皮素抗氯化镉诱导的鸡卵泡颗粒细胞氧化损伤 2011——(2.85)榭皮素抗氯化镉诱导的鸡卵泡颗粒细胞氧化 损伤 ReproductiveToxicology31 (2011) 477–485 ContentslistsavailableatScienceDirect Reproductive Toxicology journalhomepage:www.elsevier.com/locate/reproto x Quercetinattenuatescadmium-inducedoxidativedamageandapoptosisingranulosacellsfromchickenovarianfollicles YudongJia,JinxingLin,YulingMi?,CaiqiaoZhang? KeyLaboratoryofAnimalEpidemicEtiology&ImmunologicalPreventionoftheMinistryofAgriculture,DepartmentofVeterinaryMedicine,CollegeofAnimalSciences,ZhejiangUniversity,No.268KaixuanRoad,Hangzhou310029,PRChina articleinfoabstract Theattenuatingeffectofquercetinoncadmium-inducedoxidativedamageandapoptosiswasinvesti-gatedinculturedgranulosacellsfromchickenovarianf——————————————————————————————————————————————— ollicles.Resultsshowedthatexposureto5?MCdCl2inducedadecreaseingranulosacellnumberandviability,causedchromatincondensationandDNAfragmentation.Moreover,cadmiumtreatmentmarkedlyincreasedmalondialdehydelevelanddecreasedglutathioneperoxidaseandsuperoxidedismutaseactivities.Furthermore,cadmiumprovokedhigherBAXexpression,inhibitedexpressionofBCL2andX-linkedinhibitorofapoptosisprotein(XIAP)andacti-vatedcaspase-3.However,simultaneoussupplementationwith1?g/mlquercetinprotectedgranulosacellsagainstcadmium-inducedcytotoxicitythroughattenuatinglipidperoxidation,renewingantioxi-dantenzymesactivitiesandalleviatingapoptosisbymodulatingXIAP,BAXandBCL2expression,andinhibitingcaspase-3activity.Therefore,theseresultssuggestedthatquercetin,asawidelydistributeddietaryantioxidant,contributespotentiallytopreventcadmium-inducedcytotoxicityingranulosacellsthroughattenuatinglipidperoxidation,elevatingintracellularantioxidantstatusandinhibitingapoptosistoensurereproductivehealth. ? 2011 Elsevier Inc. All rights reserved. Articlehistory: Received21October2010Receivedinrevisedform27December2010 Accepted29December2010 Available online 22 January 2011Keywords:QuercetinCadmium OxidativestressApoptosis GranulosacellsChicken ——————————————————————————————————————————————— 1.Introduction Cadmiumisatoxicheavymetalthathasbeendetectedinallpartsofenvironmentandbiologicalsystem[1–3].Thismetalaccumulatesinecosystemsandenter sthefoodchainthroughenvironmentalcontaminationofsoilandwaterforanextremelylongbiologicalhalf-life.Long-termexposuretocadmiumresultsindirecttoxiceffects[4].Itstargetsoftoxicityincludepredominantlykidney,lung,liverandcardiovascularsystem,wherecadmium-inducedcelldeathleadstofunctionalde?ciencyororgandamage[5].Inaddition,cadmiumexposureadverselyaffectsboththemaleandfemalereproductivesystem,resultingindecreasedfertilityinhumanandanimals[6,7].Ithasbeenreportedthatlowlevelsofcadmiumaccumulationinsemenmayreducespermquality,whichcontributestomaleinfertilityandreducesthepregnancyrate[8].Numerousinvestigationshavealsoindicatedthatcad-miumisaknownteratogeninavian,rodent,xenopusandzebra?shembryostocausebodywalldefects,neuraltubedefects,devel-opmentaldelay,andevendeath[9–12].Furthermore,cadmiumcouldinduceendo crinedisruption,reducesteroidogenesis[13],causemorphologicalalterationsinovariangranulosacellsand ?Correspondingauthors.Tel.:+8657188982976;fax:+8657188982976. E-mailaddresses:yulingmi@zju.edu.cn(Y.Mi),cqzhang@zju.edu.cn(C.Zhang).0890-6238/$–seefrontmatter? 2011 Elsevier Inc. All rights ——————————————————————————————————————————————— reserved.doi:10.1016/j.reprotox.2010.12.057 inhibitovulationinatime-anddose-dependentmanner[6,14].Thereisgrowingevidencethatoxidativestressviareactiveoxygenspecies(ROS)generationandmitochondrialdamageareamongthefundamentalmolecularmechanismsofcadmiumtoxicity[15,16].Moreover,apoptoticmechanismsinvolvingcaspase-dependentandcaspase-independentpathwaysweredescribedfordifferentcelllinesexposedtocadmium[17,18].Inspiteofthese?ndings,thecontributionofoxidativestressandapoptoticmechanismstocadmium-inducedreproductivetoxicityawaitsfurtherdetailedclari?cation. Inrecentyears,numerousstudiesexploredthetherapeuticpropertiesofextractsfromdifferentpartsofvariousmedicinalplants.Thepositiveeffectsof?avonoidsonhumanhealthhaveattractedmoreandmoreattention.Quercetin,amemberofthe?avonoidsfamily,iswidelydistributedinfruits,vegetables,herbs,soybeans,tea,wine,etc.[19]andisthoughttohavebene?cialeffectstohumanhealth,suchasprotectingagainstosteoporosis,certainformsofcancer,pulmonaryandcardiovasculardiseasesandalsoagainstsomeagingsymptom.Manyoftheseeffectsaresupposedtoberelatedtoitsantioxidant,anti-in?ammatoryandanti-apoptosisproperties[20–23].Inthepastdecade,theantioxi-dan tactivityofquercetinhasbeengivenmuchattention.Extensivestudiesincell-freesystemshavedemonstratedthatquercetinpossessesef?cacyinscavenging——————————————————————————————————————————————— freeradicalsandthiseffectisevenstrongerthanthetraditionalantioxidantsvitaminsCandE 478Y.Jiaetal./Reproductive ———————————————————————————————————————————————