低频超声开放大鼠血脑屏障的研究

解剖科学进展 Progress of Anatomical Sciences 2007．1 3(4)：343～345 低频超声开放大鼠血脑屏障的研究 张 震 ，夏春义 ，薛一雪3，刘云会 (中国医科大学1．一院急诊科；2．二院神经外科；3．神经生物学教研室，辽宁 沈阳 110004) 【摘要】目的 探讨1MHz低频超声在不同功率、不同照射时间对大鼠脑细胞及血脑屏障的影响，寻求开放血 脑屏障的最佳条件。方法 l19只Wistar大鼠颅顶右前方开窗后超声照射，采用伊文思蓝 (EB)法血脑屏障通 透性的变化 ；应用透射电镜观察内皮细胞紧密连接的变化。结果 1MHz、12 mw照射20s或30s，照射后3 hrs，血 脑屏障通透性均显著高于对照组(P<0．05)，电镜下微血管内皮紧密连接呈开放状态，照射时间30s时，可见部分神经 元核膜变形；照射后12 hrs，与对照组相比血脑屏障通透性无明显差异(P<0．05)，且部分神经元核膜的变形无明显恢 复。1MHz、8 mW照射的各组与对照组比较血脑屏障通透性无明显差异(P<0．05)。结论 1MHz、12 mw、照射时间 20s可能是可逆性地开放血脑屏障的适宜条件。 【关键词】低频超声；血脑屏障；通透性 【中图分类号】Q189·R338 【文献标识码】A 【文章编号】1006—2947(2007)04—0343—03 The Effect of Low Frequency Ultrasound on Opening Blood——brain Barrier in Rat ZhANG Zhen ，XIA Chun—yi ，XUE Yi—xue ，LIU Yun—hui (1．Department of Emergency，the First Affiliated Hospital；2．Department of Neurosurgery，the Second Affiliated Hospital； 3．Department of Neurobiology，Basic Medical College，China Medical University，Liaoning Shenyang 1 10001 China) 【Abstract】0bjective To investigate the effect of low frequency ultrasound at l MHz with different power and illumination time on the blood—brain barrier fBBB)and the eortic neurons of rats to seek the optimum sonication condition to open BBB．Method The ultrasonic illumination was performed through the window on the anterior right part of the cranium in l19 Wistar rats，the BBB perm eability was measured by Evans blue assay and the state of the interendothelilal tight iunction of capillaries was observed with transmission electron microscope fTEM1．Results Compared with the normal control，BBB permeability increased and interendothelilal tight junction of capillaries opened 3 hours after illumination with the power of 1 2mW for 20s or 30s．but the abnorm alities of nucleo—membrane of neurons was seen while the illumination time was 30s． However,there was no obvious difference for BBB perm eability and no recovery for the abnorm al nueleo-membrane 1 2hrs after illumination，compared with the control(P标准

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